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anti-Mouse (Murine) IL21 antibody for Flow Cytometry

Recommended IL21 Antibody (supplied by: Log in to see )

Antigen
Interleukin 21 (IL21) Antibodies
  • IL-21
  • Za11
Reactivity
Mouse (Murine)
204
95
22
8
5
5
Host
Rat
161
70
35
11
5
Clonality
Monoclonal
Conjugate
This IL21 antibody is conjugated to PE
20
17
9
9
8
8
6
6
5
5
4
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
182
129
47
19
18
15
11
10
8
6
6
6
4
3
2
2
1
1
1
1
1
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Catalog No. ABIN1032366
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.29105452 ABIN1032367 FACS ICS PE Rat IgG2a kappa Log in to see mhalx21
0.29105452 ABIN1032369 FACS ICS APC Rat IgG2a kappa Log in to see FFA21
0.29105452 ABIN1032370 FACS ICS EFluor 660 Rat IgG2a kappa Log in to see FFA21
0.29105452 ABIN2680406 FACS ICS EFluor 660 Rat IgG2a kappa Log in to see mhalx21
0.0010392143 ABIN4324908 FACS Alexa Fluor 750 Rat IgG2b AA 25-146 Log in to see 149204
0.0010392143 ABIN4324907 FACS Alexa Fluor 647 Rat IgG2b AA 25-146 Log in to see 149204
0.0010392143 ABIN4324910 FACS Alexa Fluor 350 Rat IgG2b AA 25-146 Log in to see 149204
0.0010392143 ABIN4324906 FACS Alexa Fluor 700 Rat IgG2b AA 25-146 Log in to see 149204
0.0010392143 ABIN4324909 FACS Alexa Fluor 594 Rat IgG2b AA 25-146 Log in to see 149204
0.0010392143 ABIN4324911 FACS Alexa Fluor 405 Rat IgG2b AA 25-146 Log in to see 149204
0.0010392143 ABIN4896827 FACS PerCP Rat IgG2b AA 25-146 Log in to see 149204
0.0010392143 ABIN4896828 FACS PerCP Rat IgG2b AA 25-146 Log in to see 149204
0.0010392143 ABIN4896829 FACS Alexa Fluor 488 Rat IgG2b AA 25-146 Log in to see 149204
0.0010392143 ABIN4896830 FACS Alexa Fluor 488 Rat IgG2b AA 25-146 Log in to see 149204
0.0010392143 ABIN4896831 FACS PE Rat IgG2b AA 25-146 Log in to see 149204 2
0.0010392143 ABIN4896825 FACS Allophycocyanin Rat IgG2b AA 25-146 Log in to see 149204 1
0.0010392143 ABIN4896826 FACS Allophycocyanin Rat IgG2b AA 25-146 Log in to see 149204 1
0.0010392143 ABIN4896832 FACS PE Rat IgG2b AA 25-146 Log in to see 149204 2
0.0010392143 ABIN4899869 FACS WB Rat IgG2b AA 25-146 Log in to see 149204
0.0010392143 ABIN1983772 FACS WB Rat IgG2 AA 25-146 Log in to see RM0268-6G53

Top referenced anti-IL21 antibody for Flow Cytometry

Similar anti-IL21 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 40 Antibodies
Flow Cytometry (FACS) 35 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Intracellular Staining (ICS) 5 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 48 Antibodies

General

Antigen Interleukin 21 (IL21) Antibodies
Reactivity Mouse (Murine)
(204), (95), (22), (8), (5), (5)
Host Rat
(161), (70), (35), (11), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL21 antibody is conjugated to PE
(20), (17), (9), (9), (8), (8), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(182), (129), (47), (19), (18), (15), (11), (10), (8), (6), (6), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-IL21 Antibody

Target Details IL21 Application Details Handling Images
Specificity This FFA21 monoclonal antibody reacts with mouse interleukin-21 (IL-21). IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by NKT, T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1, IL-4 for Th2 and IL-17A for Th17, IL-21 is critical for TFH cell effector function. This cytokine plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells.

In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. High levels of IL-21 are present in chemically-induced colitis models. IL-21-deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules.

Preliminary data suggest that clone FFA21 recognizes a different epitope than anti-mouse IL-21 clone mhalx21 .
Cross-Reactivity (Details) This FFA21 monoclonal antibody reacts with mouse interleukin-21 (IL-21).
Characteristics This FFA21 monoclonal antibody reacts with mouse interleukin-21 (IL-21). IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by NKT, T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL- 21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1, IL-4 for Th2 and IL-17A for Th17, IL-21 is critical for TFH cell effector function. This cytokine plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. High levels of IL-21 are present in chemically-induced colitis models. IL-21-deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. Preliminary data suggest that clone FFA21 recognizes a different epitope than anti-mouse IL-21 clone mhalx21 (cat. 51-7213).
Synonyms: Synonyms: Interleukin-21
Clone FFA21
Isotype IgG2a kappa

Target Details IL21

Product Details anti-IL21 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-21 (IL21 Antibody Abstract)
Background IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by NKT, T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1, IL-4 for Th2 and IL-17A for Th17, IL-21 is critical for TFH cell effector function. This cytokine plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma, IL-21 also mediates the downregulation of Foxp3. High levels of IL-21 are present in chemically-induced colitis models. IL-21-deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules.Preliminary data suggest that clone FFA21 recognizes a different epitope than anti-mouse IL-21 clone mhalx21 (ABIN1032867).
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL21 Antibody Target Details IL21 Handling Images back to top
Application Notes Applications reported: This FFA21 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. This clone has been optimized for use with eBioscience IC Fixation and Permeabilization kit .

Applications tested: This FFA21 antibody has been tested on Th17-polarized mouse splenocytes restimulated with PMA, ionomycin and brefeldin A for 5 hours. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Comment

Excitation/Emission wavelength: 496 nm/576 nm

Restrictions For Research Use only

Handling

Product Details anti-IL21 Antibody Target Details IL21 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light sensitive material.
Storage 4 °C

Images

Product Details anti-IL21 Antibody Target Details IL21 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IL21 antibody (Interleukin 21)  (PE) (ABIN1032366) Intracellular staining of 11-day Th17-polarized BALB/c splenocytes with Anti-Mouse CD...