anti-Mouse (Murine) IL21 antibody for Flow Cytometry

Recommended IL21 Antibody (supplied by: Log in to see )

Antigen
Interleukin 21 (IL21) Antibodies
  • IL-21
  • Za11
  • interleukin 21
  • interleukin 21-like
  • IL21
  • Il21
  • LOC100344172
Epitope
AA 25-146
22
18
15
13
8
8
6
6
6
6
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
204
102
24
10
5
5
4
1
1
Host
Rat
166
70
40
11
5
Clonality
Monoclonal
Conjugate
This IL21 antibody is un-conjugated
20
15
11
10
9
8
6
6
5
5
5
5
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Western Blotting (WB)
186
133
48
39
34
26
15
13
7
6
5
4
4
4
3
2
1
1
1
Supplier
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Catalog No. ABIN4899869
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.320307 ABIN4896826 FACS Allophycocyanin Rat IgG2b AA 25-146 Log in to see 149204 1
14.320307 ABIN4896825 FACS Allophycocyanin Rat IgG2b AA 25-146 Log in to see 149204 1
14.320307 ABIN4896831 FACS PE Rat IgG2b AA 25-146 Log in to see 149204 2
14.320307 ABIN4896832 FACS PE Rat IgG2b AA 25-146 Log in to see 149204 2
14.320307 ABIN4896828 FACS PerCP Rat IgG2b AA 25-146 Log in to see 149204
14.320307 ABIN4896827 FACS PerCP Rat IgG2b AA 25-146 Log in to see 149204
14.320307 ABIN4896829 FACS Alexa Fluor 488 Rat IgG2b AA 25-146 Log in to see 149204
14.320307 ABIN4896830 FACS Alexa Fluor 488 Rat IgG2b AA 25-146 Log in to see 149204
1 ABIN4324909 FACS Alexa Fluor 594 Rat IgG2b AA 25-146 Log in to see 149204
1 ABIN4324906 FACS Alexa Fluor 700 Rat IgG2b AA 25-146 Log in to see 149204
1 ABIN4324907 FACS Alexa Fluor 647 Rat IgG2b AA 25-146 Log in to see 149204
1 ABIN4324911 FACS Alexa Fluor 405 Rat IgG2b AA 25-146 Log in to see 149204
1 ABIN4324910 FACS Alexa Fluor 350 Rat IgG2b AA 25-146 Log in to see 149204
1 ABIN4324908 FACS Alexa Fluor 750 Rat IgG2b AA 25-146 Log in to see 149204
1 ABIN4225358 CyTOF FACS WB Rat IgG2 Log in to see RM0268-6G53
1 ABIN4225361 FACS PE Rat IgG2 Log in to see RM0268-6G53
1 ABIN4225363 FACS Allophycocyanin Rat IgG2 Log in to see RM0268-6G53
1 ABIN4225367 FACS WB DyLight 680 Rat IgG2 Log in to see RM0268-6G53
1 ABIN4225403 FACS WB Alexa Fluor 405 Rat IgG2 Log in to see RM0268-6G53
1 ABIN4225359 FACS WB DyLight 350 Rat IgG2 Log in to see RM0268-6G53

General

Antigen Interleukin 21 (IL21) Antibodies
Epitope AA 25-146
(22), (18), (15), (13), (8), (8), (6), (6), (6), (6), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(204), (102), (24), (10), (5), (5), (4), (1), (1)
Host Rat
(166), (70), (40), (11), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL21 antibody is un-conjugated
(20), (15), (11), (10), (9), (8), (6), (6), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Western Blotting (WB)
(186), (133), (48), (39), (34), (26), (15), (13), (7), (6), (5), (4), (4), (4), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL21 Antibody

Target Details IL21 Application Details Handling ProductDetails: References for anti-IL21 antibody (ABIN4899869) Images
Specificity Detects mouse IL-21 in Western blots. Does not cross-react with recombinant human IL-21, recombinant mouse (rm) IL-15, or rmIL-12.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen E. coli-derived recombinant mouse IL-21, Pro25-Ser146, Accession # Q9ES17
Clone 149204
Isotype IgG2b

Target Details IL21

Product Details anti-IL21 Antibody Application Details Handling ProductDetails: References for anti-IL21 antibody (ABIN4899869) Images back to top
Antigen
Alternative Name IL-21 (IL21 Antibody Abstract)
Background Alternative Names: IL21, IL-21Za11interleukin-21, interleukin 21, interleukin-21 isoform
Gene ID 59067
UniProt Q9ES17
Research Area Immunology, Cytokines, Innate Immunity, Inflammation, Cancer, Virology
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-IL21 Antibody Target Details IL21 Handling ProductDetails: References for anti-IL21 antibody (ABIN4899869) Images back to top
Application Notes Western Blot 1 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-IL21 Antibody Target Details IL21 Application Details ProductDetails: References for anti-IL21 antibody (ABIN4899869) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

ProductDetails: References for anti-IL21 antibody (ABIN4899869)

Product Details anti-IL21 Antibody Target Details IL21 Application Details Handling Images back to top
Product cited in:

Pot, Jin, Awasthi, Liu, Lai, Madan, Sharpe, Karp, Miaw, Ho, Kuchroo: "Cutting edge: IL-27 induces the transcription factor c-Maf, cytokine IL-21, and the costimulatory receptor ICOS that coordinately act together to promote differentiation of IL-10-producing Tr1 cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 2, pp. 797-801, 2009 Method employed by authors: Neutralization (Neut) (Sample species: Mouse (Murine)).

Images

Product Details anti-IL21 Antibody Target Details IL21 Application Details Handling ProductDetails: References for anti-IL21 antibody (ABIN4899869) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Interleukin 21 (IL21) (AA 25-146) antibody (ABIN4899869) Detection of IL-21 in Activated Mouse Splenocytes by Flow Cytometry. Mouse splenocyte...
Flow Cytometry (FACS) image for anti-Interleukin 21 (IL21) (AA 25-146) antibody (ABIN4899869) Detection of IL-21 in Activated Mouse Splenocytes by Flow Cytometry. Mouse splenocyte...