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anti-Human IL3RA antibody for Enzyme Immunoassay

Recommended IL3RA Antibody (supplied by: Log in to see )

Antigen
Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
  • CD123
  • CDw123
  • Cyrl
  • hIL-3Ra
  • IL3R
  • IL3RAY
  • IL3RX
  • IL3RY
  • SUT-1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
247
53
2
1
Host
Rabbit
158
98
39
1
Clonality
Polyclonal
Conjugate
This IL3RA antibody is un-conjugated
25
23
21
13
10
6
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
182
144
55
42
35
31
30
28
16
11
10
8
4
3
3
2
1
1
1
1
1
1
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Available images

Catalog No. ABIN265478
$ 390.50
Plus shipping costs $45.00

Similar anti-IL3RA Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies 4 Antibodies
Western Blotting (WB) 3 Antibodies 14 Antibodies
ELISA 8 Antibodies 50 Antibodies
ELISA (Detection) 1 Antibodies
Flow Cytometry (FACS) 37 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunohistochemistry (IHC) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Blocking Reagent (BR) 3 Antibodies
Dot Blot (DB) 10 Antibodies

General

Antigen Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(247), (53), (2), (1)
Host Rabbit
(158), (98), (39), (1)
Clonality
Polyclonal
Conjugate This IL3RA antibody is un-conjugated
(25), (23), (21), (13), (10), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(182), (144), (55), (42), (35), (31), (30), (28), (16), (11), (10), (8), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL3RA Antibody

Target Details IL3RA Application Details Handling Images
Specificity This antibody detects endogenous levels of IL-3R protein. (region surrounding Phe587)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity Chromatography using epitope-specific immunogen

Target Details IL3RA

Product Details anti-IL3RA Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD123 / IL3RA (IL3RA Antibody Abstract)
Background Interleukin 3 (IL3), originally called Colony Forming Unit-Stimulating Activity (CFU-SA) is a multifunctional cytokine produced by activated T lymphocytes or mast cells. IL3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. It induces colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar suspended bone marrow cells. Interleukin 3 binds specifically and with high affinity to cell-surface receptors. Interleukin 3 receptors are members of the cytokine receptor superfamily. The IL3 receptor complex contains two distinct cytokine receptor subunits, ligand binding IL3 Ralpha and IL3 Rbeta. Recombinant human IL3 sRalpha (286 amino acid residue) has a predicted molecular mass of approximately 33 kDa. The alpha subunit alone binds IL3 with low affinity. The beta subunit does not bind IL3 by itself but is required for the high-affinity binding of IL3 to the heterodimeric receptor complex. The alpha subunit is also a component of the high-affinity receptor complex for IL5 and GMCSF.Synonyms: IL-3R-alpha, IL3 receptor alpha, Interleukin-3 receptor subunit alpha
Molecular Weight approx. 44 kDa
Gene ID 3563
NCBI Accession NP_002174
UniProt P26951
Research Area CD Antigens
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL3RA Antibody Target Details IL3RA Handling Images back to top
Application Notes ELISA: 1/1000-1/20000. Western Blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling back to top
Supplier Images
 image for anti-IL3RA antibody (Interleukin 3 Receptor, alpha (Low Affinity)) (ABIN265478) anti-Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) antibody
 image for anti-IL3RA antibody (Interleukin 3 Receptor, alpha (Low Affinity)) (ABIN265478) anti-Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) antibody (Image 2)