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anti-Human IL3RA antibody for Intracellular Flow Cytometry

Recommended IL3RA Antibody (supplied by: Log in to see )

Antigen
Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
  • CD123
  • CDw123
  • Cyrl
  • hIL-3Ra
  • IL3R
  • IL3RAY
  • IL3RX
  • IL3RY
  • SUT-1
Reactivity
Human
247
54
3
1
Host
Mouse
157
99
39
1
Clonality
Monoclonal
Conjugate
This IL3RA antibody is conjugated to PE-Cy7
25
23
21
13
10
6
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
181
145
55
42
35
31
30
28
17
11
10
7
4
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN2659617
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008737065 ABIN2657123 FACS ICFC Alexa Fluor 647 Mouse IgG1 kappa Log in to see 6H6 5
0.0008737065 ABIN2661494 FACS ICFC FITC Mouse IgG1 kappa Log in to see 6H6 6
0.0008737065 ABIN2659618 FACS ICFC PE-Cy7 Mouse IgG1 kappa Log in to see 6H6 10
0.0008737065 ABIN2657124 FACS ICFC Alexa Fluor 647 Mouse IgG1 kappa Log in to see 6H6 5
0.0008737065 ABIN2661495 FACS ICFC FITC Mouse IgG1 kappa Log in to see 6H6 6

Top referenced anti-IL3RA antibody for Intracellular Flow Cytometry

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Application / Reactivity Human
Blocking Reagent (BR) 3 Antibodies
Dot Blot (DB) 10 Antibodies
ELISA 50 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 146 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 33 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 28 Antibodies

General

Antigen Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
Reactivity Human
(247), (54), (3), (1)
Host Mouse
(157), (99), (39), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL3RA antibody is conjugated to PE-Cy7
(25), (23), (21), (13), (10), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
(181), (145), (55), (42), (35), (31), (30), (28), (17), (11), (10), (7), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-IL3RA Antibody

Target Details IL3RA Application Details Handling References for anti-IL3RA Antibody (ABIN2659617) Images
Cross-Reactivity Rhesus Monkey
Purification The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Clone 6H6
Isotype IgG1 kappa

Target Details IL3RA

Product Details anti-IL3RA Antibody Application Details Handling References for anti-IL3RA Antibody (ABIN2659617) Images back to top
Antigen
Alternative Name CD123 (IL3RA Antibody Abstract)
Background CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity, when CD123 associates with CDw131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL3RA Antibody Target Details IL3RA Handling References for anti-IL3RA Antibody (ABIN2659617) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details References for anti-IL3RA Antibody (ABIN2659617) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-IL3RA Antibody (ABIN2659617)

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling Images back to top
Background publications

Greer, Matthay, Kukreja, Bhakta, Nguyen, Wolters, Woodruff, Fahy, Shin: "Accumulation of BDCA1⁺ dendritic cells in interstitial fibrotic lung diseases and Th2-high asthma." in: PLoS ONE, Vol. 9, Issue 6, pp. e99084, 2014

Jin, Zhang, Wong, Kwak, van Driel, Yu: "Inhibition of breast cancer resistance protein (ABCG2) in human myeloid dendritic cells induces potent tolerogenic functions during LPS stimulation." in: PLoS ONE, Vol. 9, Issue 8, pp. e104753, 2014

Kivisäkk, Francois, Mbianda, Gandhi, Weiner, Khoury: "Effect of natalizumab treatment on circulating plasmacytoid dendritic cells: a cross-sectional observational study in patients with multiple sclerosis." in: PLoS ONE, Vol. 9, Issue 7, pp. e103716, 2014

Wieland, Takahashi, Boyd, Whitten-Bauer, Ngo, de la Torre, Chisari: "Human plasmacytoid dendritic cells sense lymphocytic choriomeningitis virus-infected cells in vitro." in: Journal of virology, Vol. 88, Issue 1, pp. 752-7, 2013

Charles, Hardwick, Daugas, Illei, Rivera: "Basophils and the T helper 2 environment can promote the development of lupus nephritis." in: Nature medicine, Vol. 16, Issue 6, pp. 701-7, 2010

Martín-Gayo, Sierra-Filardi, Corbí, Toribio: "Plasmacytoid dendritic cells resident in human thymus drive natural Treg cell development." in: Blood, Vol. 115, Issue 26, pp. 5366-75, 2010

Herling, Teitell, Shen, Medeiros, Jones: "TCL1 expression in plasmacytoid dendritic cells (DC2s) and the related CD4+ CD56+ blastic tumors of skin." in: Blood, Vol. 101, Issue 12, pp. 5007-9, 2003

Sun, Woodcock, Rapoport, Stomski, Korpelainen, Bagley, Goodall, Smith, Gamble, Vadas, Lopez: "Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist." in: Blood, Vol. 87, Issue 1, pp. 83-92, 1996

Miyajima, Mui, Ogorochi, Sakamaki: "Receptors for granulocyte-macrophage colony-stimulating factor, interleukin-3, and interleukin-5." in: Blood, Vol. 82, Issue 7, pp. 1960-74, 1993

Images

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling References for anti-IL3RA Antibody (ABIN2659617) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IL3RA antibody (Interleukin 3 Receptor, alpha (Low Affinity))  (PE-Cy7) (ABIN2659617) anti-Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) antibody (PE-Cy7)