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anti-Human IL3RA antibody for Immunohistochemistry (Frozen Sections)

Recommended IL3RA Antibody (supplied by: Log in to see )

Antigen
Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
  • CD123
  • CDw123
  • Cyrl
  • hIL-3Ra
  • IL3R
  • IL3RAY
  • IL3RX
  • IL3RY
  • SUT-1
Reactivity
Human
247
54
3
1
Host
Mouse
157
99
39
1
Clonality
Monoclonal
Conjugate
This IL3RA antibody is un-conjugated
25
23
21
13
10
6
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
181
144
55
42
34
31
30
27
17
11
10
8
4
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN1302506
$ 231.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088234665 ABIN269380 FACS IHC (fro) IHC WB Mouse IgG1 kappa Log in to see 6H6 2
0.00088234665 ABIN1176839 IHC (fro) FACS IP WB PE Mouse IgG1 Log in to see 6H6 3
0.00088234665 ABIN1558083 IHC (fro) FACS IP WB APC Mouse IgG1 Log in to see 6H6 3
0.00088234665 ABIN205441 FACS IHC (fro) IP WB Mouse IgG1, kappa Log in to see 6H6
0.00088234665 ABIN476008 FACS IHC (fro) IHC (p) Mouse IgG1, kappa Log in to see 6H6
0.00088234665 ABIN280627 FACS IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see 6H6
0.00088234665 ABIN1174728 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 19-157 Log in to see Polyclonal
0.00088234665 ABIN327329 FACS IHC (fro) IP WB Mouse IgG1, kappa Log in to see 6H6
0.00088234665 ABIN1959961 IHC (fro) FACS IP WB Mouse IgG1 Log in to see 6H6
0.00088234665 ABIN3199431 FACS IHC (fro) IP WB APC Mouse IgG1 Log in to see 6H6
0.00088234665 ABIN3199432 FACS IHC (fro) IP WB Biotin Mouse IgG1 Log in to see 6H6
0.00088234665 ABIN3199433 FACS IHC (fro) IP WB FITC Mouse IgG1 Log in to see 6H6
0.00088234665 ABIN3199434 FACS IHC (fro) IP WB PE Mouse IgG1 Log in to see 6H6
0.00088234665 ABIN2478330 IHC (fro) FACS IP WB Mouse IgG1 Log in to see 6H6 3
0.00088234665 ABIN3199430 FACS IHC (fro) IP WB Mouse IgG1 Log in to see 6H6
0.00088234665 ABIN4225827 FACS IHC IHC (fro) WB DyLight 680 Mouse IgG1 kappa Log in to see 6H6
0.00088234665 ABIN4225826 FACS IHC IHC (fro) WB DyLight 755 Mouse IgG1 kappa Log in to see 6H6
0.00088234665 ABIN4225828 FACS IHC IHC (fro) WB DyLight 405LS Mouse IgG1 kappa Log in to see 6H6
0.00088234665 ABIN4225824 FACS IHC IHC (fro) WB DyLight 350 Mouse IgG1 kappa Log in to see 6H6
0.00088234665 ABIN4225825 FACS IHC IHC (fro) WB PerCP Mouse IgG1 kappa Log in to see 6H6

Top referenced anti-IL3RA antibody for Immunohistochemistry (Frozen Sections)

Similar anti-IL3RA Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 3 Antibodies
Dot Blot (DB) 10 Antibodies
ELISA 50 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 145 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 42 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 33 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunoprecipitation (IP) 28 Antibodies

General

Antigen Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
Reactivity Human
(247), (54), (3), (1)
Host Mouse
(157), (99), (39), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL3RA antibody is un-conjugated
(25), (23), (21), (13), (10), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(181), (144), (55), (42), (34), (31), (30), (27), (17), (11), (10), (8), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL3RA Antibody

Target Details IL3RA Application Details Handling References for anti-IL3RA Antibody (ABIN1302506) Images
Specificity The mouse monoclonal antibody 6H6 recognizes CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor.
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells
Clone 6H6
Isotype IgG1

Target Details IL3RA

Product Details anti-IL3RA Antibody Application Details Handling References for anti-IL3RA Antibody (ABIN1302506) Images back to top
Antigen
Alternative Name IL3RA (IL3RA Antibody Abstract)
Background CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.
Gene ID 3563
Research Area Signaling, Innate Immunity, Cytokines
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL3RA Antibody Target Details IL3RA Handling References for anti-IL3RA Antibody (ABIN1302506) Images back to top
Application Notes Working concentrations should be determined by the investigator.I t is recommended that the user titrates the reagent for use in the particular testing system.

Working concentrations should be determined by the investigator.
Comment

CD and Related Antigens (Human), Cytokine Signaling (Human)

Restrictions For Research Use only

Handling

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details References for anti-IL3RA Antibody (ABIN1302506) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-IL3RA Antibody (ABIN1302506)

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling Images back to top
Product cited in:

Martín-Gayo, Sierra-Filardi, Corbí, Toribio: "Plasmacytoid dendritic cells resident in human thymus drive natural Treg cell development." in: Blood, Vol. 115, Issue 26, pp. 5366-75, 2010

Herling, Teitell, Shen, Medeiros, Jones: "TCL1 expression in plasmacytoid dendritic cells (DC2s) and the related CD4+ CD56+ blastic tumors of skin." in: Blood, Vol. 101, Issue 12, pp. 5007-9, 2003