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anti-Human IL3RA antibody for Neutralization

Recommended IL3RA Antibody (supplied by: Log in to see )

Antigen
Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
  • CD123
  • CDw123
  • Cyrl
  • hIL-3Ra
  • IL3R
  • IL3RAY
  • IL3RX
  • IL3RY
  • SUT-1
Reactivity
Human
247
54
3
1
Host
Mouse
157
99
39
1
Clonality
Monoclonal
Conjugate
This IL3RA antibody is un-conjugated
25
23
21
13
10
6
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Neutralization (Neut)
181
145
55
42
35
31
30
28
17
11
10
8
3
3
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1176902
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008737065 ABIN2688934 BR FACS IP Neut WB Mouse IgG2a, kappa Log in to see 7G3 1
0.0008737065 ABIN1983291 Neut IHC WB Mouse IgG1 AA 20-305 Log in to see MM0403-11A23
0.0008737065 ABIN4325131 BP IHC IHC (fro) Neut WB Mouse IgG1 Log in to see MM0403-11A23

Top referenced anti-IL3RA antibody for Neutralization

  • Human Monoclonal IL3RA Primary Antibody for FACS, Neut - ABIN1176902 (1 Publications): Sun, Woodcock, Rapoport, Stomski, Korpelainen, Bagley, Goodall, Smith, Gamble, Vadas, Lopez: Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist. in Blood 1996 (PubMed)

Similar anti-IL3RA Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 3 Antibodies
Dot Blot (DB) 10 Antibodies
ELISA 50 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 146 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 33 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 28 Antibodies

General

Antigen Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
Reactivity Human
(247), (54), (3), (1)
Host Mouse
(157), (99), (39), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL3RA antibody is un-conjugated
(25), (23), (21), (13), (10), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Neutralization (Neut)
(181), (145), (55), (42), (35), (31), (30), (28), (17), (11), (10), (8), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL3RA Antibody

Target Details IL3RA Application Details Handling References for anti-IL3RA Antibody (ABIN1176902) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Human IL-3Ra-transfected cells
Clone 7G3
Isotype IgG2a, kappa

Target Details IL3RA

Product Details anti-IL3RA Antibody Application Details Handling References for anti-IL3RA Antibody (ABIN1176902) Images back to top
Antigen
Alternative Name CD123 (IL3RA Antibody Abstract)
Background Reacts with human CD123, the 70 kD IL-3 receptor alpha chain (IL-3Ralpha), which associates with the 120-140 kD beta subunit. The beta chain is shared with the receptors for interleukins IL-5 and GM-CSF. IL-3Ralpha is expressed on hematopoietic progenitors and plays an important role in hematopoietic progenitor cell growth and differentiation. This antibody has been reported to block the binding of 125I-IL-3 to high and low affinity IL-3 receptors. In functional experiments, this antibody was found to inhibit acute myeloid leukemia cell proliferation, basophil histamine release, endothelial cell-mediated IL-8 secretion, and neutrophil transmigration. This antibody has been reported to be useful for immunoprecipitation, western blot and immunofluorescent staining for flow cytometry. At the Fifth HLDA Workshop, the human IL-3 receptor was designated CD123. This antibody is routinely tested by LAL for endotoxin level and by immunofluorescent staining and flow cytometry.
Synonyms: IL-3 Receptor alpha chain
Research Area Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL3RA Antibody Target Details IL3RA Handling References for anti-IL3RA Antibody (ABIN1176902) Images back to top
Application Notes This antibody has been found to block the binding of 125I-IL-3 to high and low affinity IL-3 receptors and can neutralize IL-3 bioactivity. In a TF-1 cell proliferation assay, this antibody (at ~0.5 µg/ml) was found to inhibit by 50% the proliferation induced by 0.3 ng/ml (~1 U/ml) of human IL-3. In functional experiments, the 7G3 antibody was found to inhibit acute myeloid leukemia cell proliferation, basophil histamine release, endothelial cell-mediated IL-8 secretion, and neutrophil transmigration. In various formats, i.e., purified with azide (Cat. No. 554527), biotinylated (Cat. No. 554528), and phycoerythrin-labeled (Cat. No. 554529), the 7G3 antibody is useful for applications such as immunoprecipitation, Western blotting, and immunofluorescent labeling of cells for flow cytometric analysis.
Restrictions For Research Use only

Handling

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details References for anti-IL3RA Antibody (ABIN1176902) Images back to top
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL3RA Antibody (ABIN1176902)

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling Images back to top
Product cited in:

Sun, Woodcock, Rapoport, Stomski, Korpelainen, Bagley, Goodall, Smith, Gamble, Vadas, Lopez: "Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist." in: Blood, Vol. 87, Issue 1, pp. 83-92, 1996