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|Application / Reactivity||Mouse (Murine)|
|ELISA (Detection)||1 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||16 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Immunofluorescence (IF)||2 Antibodies|
|Immunohistochemistry (IHC)||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||4 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Western Blotting (WB)||13 Antibodies|
|Antigen||Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This IL3RA antibody is conjugated to PE Alternatives|
ELISA (Detection), Intracellular Flow Cytometry (ICFC)
|5 references available|
|Supplier||Log in to see|
Product Details anti-IL3RA AntibodyTarget Details IL3RA Application Details Handling References for anti-IL3RA Antibody (ABIN2662992) Images
|Purification||The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.|
Target Details IL3RAProduct Details anti-IL3RA Antibody Application Details Handling References for anti-IL3RA Antibody (ABIN2662992) Images back to top
|Alternative Name||CD123 (IL3RA Antibody Abstract)|
|Background||CD123 is a 70 kD α chain subunit of the IL-3 receptor (IL-3R α). It is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL-3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either the common β chain (CDw131) or the IL-3R β chain (AIC2A) to form high-affinity heterodimeric IL-3 receptors. CDw131 can complex with the α subunits of the mouse IL-3R, IL-5R and GM-CSFR to form high-affinity receptors, while the IL-3 R β subunit is specific for IL-3 but binds with low affinity. IL-3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. The 5B11 antibody does not block binding of IL-3 to the high affinity IL-3 receptor.|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-IL3RA Antibody Target Details IL3RA Handling References for anti-IL3RA Antibody (ABIN2662992) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL3RA Antibody Target Details IL3RA Application Details References for anti-IL3RA Antibody (ABIN2662992) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Protect from prolonged exposure to light. Do not freeze.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C.|
References for anti-IL3RA Antibody (ABIN2662992)Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling Images back to top
Cervantes-Barragan, Züst, Weber et al.: "Control of coronavirus infection through plasmacytoid dendritic-cell-derived type I interferon." in: Blood, Vol. 109, Issue 3, pp. 1131-7, 2007 (PubMed).
Miyajima, Mui, Ogorochi et al.: "Receptors for granulocyte-macrophage colony-stimulating factor, interleukin-3, and interleukin-5." in: Blood, Vol. 82, Issue 7, pp. 1960-74, 1993 (PubMed).
Ichihara, Hara, Takagi et al.: "Impaired interleukin-3 (IL-3) response of the A/J mouse is caused by a branch point deletion in the IL-3 receptor alpha subunit gene." in: The EMBO journal, Vol. 14, Issue 5, pp. 939-50, 1995 (PubMed).
Mueller, Chen, Schwartz et al.: "Subset of CD4+ T cell clones expressing IL-3 receptor alpha-chains uses IL-3 as a cofactor in autocrine growth." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 7, pp. 3014-27, 1994 (PubMed).
Itoh, Yonehara, Schreurs et al.: "Cloning of an interleukin-3 receptor gene: a member of a distinct receptor gene family." in: Science (New York, N.Y.), Vol. 247, Issue 4940, pp. 324-7, 1990 (PubMed).
ImagesProduct Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling References for anti-IL3RA Antibody (ABIN2662992) back to top