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anti-Mouse (Murine) IL3RA antibody for Immunofluorescence

Recommended IL3RA Antibody (supplied by: Log in to see )

Antigen
Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
  • CD123
  • CDw123
  • Cyrl
  • hIL-3Ra
  • IL3R
  • IL3RAY
  • IL3RX
  • IL3RY
  • SUT-1
Reactivity
Mouse (Murine)
246
53
3
1
Host
Rat
156
99
38
1
Clonality
Monoclonal
Conjugate
This IL3RA antibody is un-conjugated
25
22
21
13
10
6
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)
179
144
55
41
34
30
29
28
17
11
10
8
4
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN254529
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010843398 ABIN2478332 FACS IF Rat IgG2a Log in to see 5B11 3
0.0010843398 ABIN4325133 FACS ICC IF DyLight 350 Rat IgG2a Log in to see 5B11
0.0010843398 ABIN4325134 FACS ICC IF DyLight 405 Rat IgG2a Log in to see 5B11
0.0010843398 ABIN4325136 FACS ICC IF PE Rat IgG2a Log in to see 5B11
0.0010843398 ABIN4325137 FACS ICC IF PerCP Rat IgG2a Log in to see 5B11
0.0010843398 ABIN4325138 FACS ICC IF Allophycocyanin Rat IgG2a Log in to see 5B11
0.0010843398 ABIN4325139 FACS ICC IF DyLight 755 Rat IgG2a Log in to see 5B11
0.0010843398 ABIN4325140 FACS ICC IF DyLight 680 Rat IgG2a Log in to see 5B11
0.0010843398 ABIN4325141 FACS ICC IF DyLight 488 Rat IgG2a Log in to see 5B11
0.0010843398 ABIN4325143 FACS ICC IF DyLight 405LS Rat IgG2a Log in to see 5B11
0.0010843398 ABIN4325161 FACS ICC IF Alexa Fluor 405 Rat IgG2a Log in to see 5B11
0.0010843398 ABIN4325162 FACS ICC IF Alexa Fluor 488 Rat IgG2a Log in to see 5B11
0.0010843398 ABIN4325163 FACS ICC IF Alexa Fluor 647 Rat IgG2a Log in to see 5B11
0.0010843398 ABIN4325164 FACS ICC IF Alexa Fluor 700 Rat IgG2a Log in to see 5B11

Top referenced anti-IL3RA antibody for Immunofluorescence

Similar anti-IL3RA Antibodies

Application / Reactivity Mouse (Murine)
ELISA 8 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 37 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunohistochemistry (IHC) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 14 Antibodies

General

Antigen Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
Reactivity Mouse (Murine)
(246), (53), (3), (1)
Host Rat
(156), (99), (38), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL3RA antibody is un-conjugated
(25), (22), (21), (13), (10), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)
(179), (144), (55), (41), (34), (30), (29), (28), (17), (11), (10), (8), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL3RA Antibody

Target Details IL3RA Application Details Handling Images
Specificity NB100-63384 recognizes murine CD123, which is the 70kD alpha chain of the interleukin-3 receptor (IL-3R). CD123 is expressed on haematopoietic progenitors and most myeloid cells including basophils and mast cells. Certain mouse strains including A/J, AKR/J and NZB/B1NJ are reported to have low levels of CD123 expression due to aberrant splicing of the IL-3R alpha subunit gene. In mouse, CD123 can associate with either the common beta subunit CDw131 or with the murine IL-3 beta subunit to form a high affinity receptor for IL-3. IL-3 can bind to CD123 itself but with low affinity. Binding of IL-3 to the IL-3 receptor complex induces the proliferation and differentiation of haematopoietic cells. Clone 5B11 does not block the binding of IL-3 to the high affinity IL-3 receptor.
Purification Protein G purified
Immunogen CTLL cells transfected with mouse CD123.
Clone 5B11
Isotype IgG2a

Target Details IL3RA

Product Details anti-IL3RA Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-3 R alpha/cd123 (IL3RA Antibody Abstract)
Background Alternate Names: Anti-CD123 antibody, Anti-hIL3Ra antibody, Anti-IL3R antibody, Anti-IL3RAY antibody,Anti-IL3RX antibody, Anti-IL3RY antibody, Anti-MGC34174 antibody.
Gene Symbol: IL3RA
Gene ID 3563
Research Area CD Antigens
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL3RA Antibody Target Details IL3RA Handling Images back to top
Application Notes Recommended dilutions: Flow Cytometry 1:10-1:50, Immunocytochemistry/Immunofluorescence 1:10-1:2000
Restrictions For Research Use only

Handling

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline, pH 7.4, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.