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anti-Human IL4 Receptor antibody for Enzyme Immunoassay

Recommended IL4 Receptor Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 Receptor (IL4R) Antibodies
  • CD124
  • IL-4RA
  • il-4ra
  • IL4
  • Il4r
  • IL4R
  • IL4RA
  • Il4ra
  • zgc:113063
Epitope
Extracellular Domain
10
9
9
9
5
5
4
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
92
33
7
Host
Mouse
54
45
12
6
1
Clonality
Monoclonal
Conjugate
This IL4 Receptor antibody is un-conjugated
6
5
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
61
52
31
14
13
9
8
6
6
3
3
3
3
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN112606
$ 665.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN2717274 EIA WB Rabbit IgG Log in to see Polyclonal
0.0008867729 ABIN2717273 EIA WB Rabbit IgG Log in to see Polyclonal
0.0008867729 ABIN2717275 EIA WB Rabbit IgG Log in to see Polyclonal

Similar anti-IL4 Receptor Antibodies

Application / Reactivity Human
ELISA 40 Antibodies
ELISA (Capture) 3 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 24 Antibodies
Functional Studies (Func) 4 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 12 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Intracellular Flow Cytometry (ICFC) 4 Antibodies
Neutralization (Neut) 4 Antibodies
Western Blotting (WB) 54 Antibodies

General

Antigen Interleukin 4 Receptor (IL4R) Antibodies
Epitope Extracellular Domain
(10), (9), (9), (9), (5), (5), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(92), (33), (7)
Host Mouse
(54), (45), (12), (6), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL4 Receptor antibody is un-conjugated
(6), (5), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(61), (52), (31), (14), (13), (9), (8), (6), (6), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-IL4 Receptor Antibody

Target Details IL4 Receptor Application Details Handling Images
Specificity This antibody is specific for Interleukin 4 Receptor, the transmembrane ligand-binding subunit of the heterodimeric IL-4 receptor complex. This antibody blocks cell surface IL-4 receptor mediated bioactivities induced by IL-4 or IL-13. An ND50 was observed at approximately 5-20 ng/mL in the presence of 0.2 ng/mL of IL-4 using the factor dependant Human erythroleukemic cell line, TF-1.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity chromatography on Protein A
Immunogen Sf 21-derived, recombinant Human interleukin 4 Receptor, extracellular domain.
Clone 25463 (49-20)
Isotype IgG1

Target Details IL4 Receptor

Product Details anti-IL4 Receptor Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD124 / IL4R (IL4R Antibody Abstract)
Background IL4 Receptor alpha (IL4RA) is the alpha chain of the interleukin 4 receptor which binds to both interleukin 4 and interleukin 13 to regulate IgE production, chemokine and mucus production at sites of allergic inflammmation. The IL4 response is involved in promoting Th2 differentiation. The secreted extracellular domain of IL4R alpha, called sIL4R alpha, can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. It has no signaling abilities. Allelic variations in the IL4RA gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.Synonyms: 582J2.1, IL-4R-alpha, IL4 Receptor alpha, IL4RA, Interleukin-4 receptor alpha chain
Gene ID 3566
NCBI Accession NP_000409
UniProt P24394
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Handling Images back to top
Application Notes Flow Cytometry: 1/20 - 1/80. ELISA: 1/500 - 1/2000. Western Blot: 1/250 - 1/1000, Will detect human IL-4 R under non-reducing conditions. Functional Assays. Immunohistochemistry on frozen sections: 1/40 - 1/125.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide, 10 % Trehalose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.