anti-Human IL4 Receptor antibody for Immunohistochemistry

Recommended IL4 Receptor Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 Receptor (IL4R) Antibodies
  • IL4R
  • il-4ra
  • zgc:113063
  • CD124
  • IL-4RA
  • IL4RA
  • Il4ra
  • IL4
  • Il4r
  • interleukin 4 receptor
  • interleukin 4 receptor, alpha
  • IL4R
  • il4r
  • LOC100220266
  • Il4r
  • Il4ra
Epitope
AA 24-232
22
20
12
11
9
7
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
127
60
10
Host
Mouse
73
64
25
14
1
Clonality
Monoclonal
Conjugate
This IL4 Receptor antibody is un-conjugated
11
9
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
95
59
56
31
28
12
11
8
6
3
3
3
3
2
2
2
2
2
1
1
Supplier
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Catalog No. ABIN4899803
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.894592 ABIN4899804 CyTOF FACS IHC Neut WB Mouse IgG2a AA 24-232 Log in to see 25463 15
1 ABIN3182448 ELISA IF IHC WB Rabbit IgG pTyr497 Log in to see Polyclonal
1 ABIN4325317 IHC IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN3185178 ELISA IHC Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN260346 FACS IHC (p) IHC ELISA WB Mouse IgG1 Log in to see 49-20
1 ABIN1453491 IHC ELISA Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN4225972 BP IHC IHC (p) Neut WB Mouse IgG1 Log in to see MM0405-4Y44
1 ABIN4325316 IHC IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN2884643 IHC ELISA Rabbit IgG AA 205-254 Log in to see Polyclonal
1 ABIN5598162 IHC IHC (p) WB Alexa Fluor 405 Mouse IgG1 Log in to see MM0405-4Y44
1 ABIN5598163 IHC IHC (p) WB Alexa Fluor 488 Mouse IgG1 Log in to see MM0405-4Y44
1 ABIN5598164 IHC IHC (p) WB Alexa Fluor 647 Mouse IgG1 Log in to see MM0405-4Y44
1 ABIN5598166 IHC IHC (p) WB Biotin Mouse IgG1 Log in to see MM0405-4Y44
1 ABIN5598165 IHC IHC (p) WB Alexa Fluor 700 Mouse IgG1 Log in to see MM0405-4Y44
1 ABIN5598167 IHC IHC (p) WB DyLight 350 Mouse IgG1 Log in to see MM0405-4Y44
1 ABIN5598168 IHC IHC (p) WB DyLight 405 Mouse IgG1 Log in to see MM0405-4Y44
1 ABIN5598169 IHC IHC (p) WB DyLight 488 Mouse IgG1 Log in to see MM0405-4Y44
1 ABIN5598170 IHC IHC (p) WB DyLight 550 Mouse IgG1 Log in to see MM0405-4Y44
1 ABIN5598171 IHC IHC (p) WB DyLight 650 Mouse IgG1 Log in to see MM0405-4Y44
1 ABIN5598172 IHC IHC (p) WB DyLight 680 Mouse IgG1 Log in to see MM0405-4Y44

General

Antigen Interleukin 4 Receptor (IL4R) Antibodies
Epitope AA 24-232
(22), (20), (12), (11), (9), (7), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(127), (60), (10)
Host Mouse
(73), (64), (25), (14), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL4 Receptor antibody is un-conjugated
(11), (9), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
(95), (59), (56), (31), (28), (12), (11), (8), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Pubmed 15 references available
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Product Details anti-IL4 Receptor Antibody

Target Details IL4 Receptor Application Details Handling ProductDetails: References for anti-IL4 Receptor antibody (ABIN4899803) Images
Specificity Detects human IL-4 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-9 R, recombinant mouse IL-4 R, rhIL-5 R alpha , rhIL-5 R beta , rhIL-13 R alpha 1, or rhIL-13 R alpha 2 is observed.
Purification Protein A or G purified from ascites
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-4 R, Gly24-His232, Accession # P24394
Clone 25463
Isotype IgG2a

Target Details IL4 Receptor

Product Details anti-IL4 Receptor Antibody Application Details Handling ProductDetails: References for anti-IL4 Receptor antibody (ABIN4899803) Images back to top
Antigen
Alternative Name IL-4 R alpha (IL4R Antibody Abstract)
Background Alternative Names: CD124, CD124 antigen, IL-4 receptor subunit alpha, IL4R, IL4R alpha, IL-4R subunit alpha, IL-4RA, IL4RACD124, IL-4R-alpha, interleukin 4 receptor, interleukin-4 receptor alpha chain, interleukin-4 receptor subunit alpha
Gene ID 3566
UniProt P24394
Pathways JAK-STAT Signaling, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Handling ProductDetails: References for anti-IL4 Receptor antibody (ABIN4899803) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunohistochemistry 8-25 μg/mL
Neutralization 3-6 ng/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Application Details ProductDetails: References for anti-IL4 Receptor antibody (ABIN4899803) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

ProductDetails: References for anti-IL4 Receptor antibody (ABIN4899803)

Product Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Application Details Handling Images back to top
Product cited in:

Reece, Gauvreau, Sehmi, Denburg: "IL-4 and IL-13 differentially regulate TLR-induced eosinophil-basophil differentiation of cord blood CD34+ progenitor cells." in: PLoS ONE, Vol. 9, Issue 6, pp. e100734, 2014 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Biancheri, Di Sabatino, Ammoscato, Facciotti, Caprioli, Curciarello, Hoque, Ghanbari, Joe-Njoku, Giuffrida, Rovedatti, Geginat, Corazza, MacDonald: "Absence of a role for interleukin-13 in inflammatory bowel disease." in: European journal of immunology, Vol. 44, Issue 2, pp. 370-85, 2014

Boulaftali, Hess, Getz, Cholka, Stolla, Mackman, Owens, Ware, Kahn, Bergmeier: "Platelet ITAM signaling is critical for vascular integrity in inflammation." in: The Journal of clinical investigation, Vol. 123, Issue 2, pp. 908-16, 2013 Method employed by authors: Binding Studies (Bind) (Sample species: Human).

Sarangthem, Cho, Bae, Singh, Kim, Kim, Jeon, Lee, Park: "Construction and application of elastin like polypeptide containing IL-4 receptor targeting peptide." in: PLoS ONE, Vol. 8, Issue 12, pp. e81891, 2013 Method employed by authors: Blocking Peptide (BP) (Sample species: Human).

Volonté, Di Tomaso, Spinelli, Todaro, Sanvito, Albarello, Bissolati, Ghirardelli, Orsenigo, Ferrone, Doglioni, Stassi, Dellabona, Staudacher, Parmiani, Maccalli: "Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 1, pp. 523-32, 2013 Method employed by authors: Immunofluorescence (IF) (Sample species: Human).

Amano, Yamashita, Kojima, Yoshino, Tanaka, Sugamura, Takeshita: "Hrs recognizes a hydrophobic amino acid cluster in cytokine receptors during ubiquitin-independent endosomal sorting." in: The Journal of biological chemistry, Vol. 286, Issue 17, pp. 15458-72, 2011 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Thors, Bergh, Persson, Hammarsten, Stattin, Egevad, Granfors, Fowler: "Fatty acid amide hydrolase in prostate cancer: association with disease severity and outcome, CB1 receptor expression and regulation by IL-4." in: PLoS ONE, Vol. 5, Issue 8, pp. e12275, 2010 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Hu, Fatma, Cao, Grunstein, Nino, Grumbach, Grunstein: "Th2 cytokine-induced upregulation of 11beta-hydroxysteroid dehydrogenase-1 facilitates glucocorticoid suppression of proasthmatic airway smooth muscle function." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 296, Issue 5, pp. L790-803, 2009 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Eyerich, Pennino, Scarponi, Foerster, Nasorri, Behrendt, Ring, Traidl-Hoffmann, Albanesi, Cavani: "IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response." in: The Journal of allergy and clinical immunology, Vol. 123, Issue 1, pp. 59-66.e4, 2009 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Aoudjehane, Pissaia, Scatton, Podevin, Massault, Chouzenoux, Soubrane, Calmus, Conti: "Interleukin-4 induces the activation and collagen production of cultured human intrahepatic fibroblasts via the STAT-6 pathway." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 88, Issue 9, pp. 973-85, 2008 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).

Aoudjehane, Podevin, Scatton, Jaffray, Dusanter-Fourt, Feldmann, Massault, Grira, Bringuier, Dousset, Chouzenoux, Soubrane, Calmus, Conti: "Interleukin-4 induces human hepatocyte apoptosis through a Fas-independent pathway." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 21, Issue 7, pp. 1433-44, 2007 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).

Hurst, McLoughlin, Monslow, Owens, Morgan, Fuller, Topley, Jones: "Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 9, pp. 5244-51, 2002 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Hirst, Hallsworth, Peng, Lee et al.: "Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor ..." in: American journal of respiratory and critical care medicine, Vol. 165, Issue 8, pp. 1161-71, 2002 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Albanesi, Scarponi, Sebastiani, Cavani, Federici, Sozzani, Girolomoni: "A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases." in: Journal of leukocyte biology, Vol. 70, Issue 4, pp. 617-23, 2001 Method employed by authors: Neutralization (Neut) (Sample species: Human).

de Lavareille, Roufosse, Schandené, Stordeur, Cogan, Goldman: "Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo." in: European journal of immunology, Vol. 31, Issue 4, pp. 1037-46, 2001 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Images

Product Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Application Details Handling ProductDetails: References for anti-IL4 Receptor antibody (ABIN4899803) back to top
Supplier Images
Neutralization (Neut) image for anti-Interleukin 4 Receptor (IL4R) (AA 24-232) antibody (ABIN4899803) Cell Proliferation Induced by IL-4 and Neutralization by Human IL-4 R alpha Antibody....
Flow Cytometry (FACS) image for anti-Interleukin 4 Receptor (IL4R) (AA 24-232) antibody (ABIN4899803) Detection of IL-4 R alpha in Daudi Human Cell Line by Flow Cytometry. Daudi human Bur...
Immunohistochemistry (IHC) image for anti-Interleukin 4 Receptor (IL4R) (AA 24-232) antibody (ABIN4899803) IL-4 R alpha in Human Lymph Node. IL-4 R alpha was detected in immersion fixed paraff...
Neutralization (Neut) image for anti-Interleukin 4 Receptor (IL4R) (AA 24-232) antibody (ABIN4899803) Cell Proliferation Induced by IL-4 and Neutralization by Human IL-4 R alpha Antibody....
Flow Cytometry (FACS) image for anti-Interleukin 4 Receptor (IL4R) (AA 24-232) antibody (ABIN4899803) Detection of IL-4 R alpha in Daudi Human Cell Line by Flow Cytometry. Daudi human Bur...
Immunohistochemistry (IHC) image for anti-Interleukin 4 Receptor (IL4R) (AA 24-232) antibody (ABIN4899803) IL-4 R alpha in Human Lymph Node. IL-4 R alpha was detected in immersion fixed paraff...