We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Mouse (Murine)|
|ELISA (Detection)||2 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||2 Antibodies|
|Flow Cytometry (FACS)||9 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (IF)||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||2 Antibodies|
|Immunohistochemistry (IHC)||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Neutralization (Neut)||4 Antibodies|
|Western Blotting (WB)||17 Antibodies|
|Antigen||Interleukin 4 Receptor (IL4R) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This IL4 Receptor antibody is conjugated to Biotin Alternatives|
Flow Cytometry (FACS), Immunoprecipitation (IP), Neutralization (Neut), ELISA
|4 references available|
|Supplier||Log in to see|
Product Details anti-IL4 Receptor AntibodyTarget Details IL4 Receptor Application Details Handling References for anti-IL4 Receptor Antibody (ABIN2688938) Images
The mIL4R-M1 monoclonal antibody specifically binds to CD124 which is also known as the α subunit of the mouse Interleukin-4 Receptor (IL-4Rα). The mouse IL-4Rα is a 140 kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and nonhematopoietic cells and cell lines. The cell surface IL-4Rα chain binds IL-4 with high affinity and associates with either the common γ chain (IL-4Rα/γc, aka, type I IL-4R) or the IL-13 receptor alpha subunit (IL-4Rα/IL-13Rα, aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes. The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R binds and transduces signals from either IL-4 or IL-13. The mIL4R-M1 antibody blocks IL-4 binding to cells and is reported to be a potent inhibitor of IL-4's biological activities. The mIL4R-M1 antibody also recognizes naturally-occurring, soluble truncated forms of IL-4Rα (sIL-4R) that result either from enzymatic cleavage of the cell surface extracellular IL-4Rα domain or from differential mRNAsplicing and secretion by cells. These sIL-4R retain their high-affinity ligand binding domain and appear to either enhance or inhibit IL-4-mediated functions depending on the relative local levels of IL-4 and sIL-4R. Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified mouse BD Fc Block™ (Cat. No. 553142) to block mouse Fc receptors. The cells were then stained with biotinylated mIL4R-M1 (0.125 μg/10^6 cells) followed by streptavidin phycoerythrin (0.015 μg, Cat. No. 554061) and FITC anti-mouse B220 (0.06 μg, Cat. No. 553088) and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells (with the light-scattering characteristics of viable lymphocytes) are shown in the two-color dot plot (right panel). Staining with the biotinylated mIL4R-M1 antibody (right panel) is compared to staining derived with a biotinylated rat IgG2a immunoglobulin isotype control antibody (0.125 μg, Cat. No. 553928) that is shown in the left panel.
BD Pharmingen™ Biotin Rat Anti-Mouse CD124 - Biotin - Clone mIL4R-M1 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Mouse CTLL-19.4 T Cell Line|
Target Details IL4 ReceptorProduct Details anti-IL4 Receptor Antibody Application Details Handling References for anti-IL4 Receptor Antibody (ABIN2688938) Images back to top
|Alternative Name||CD124 (IL4R Antibody Abstract)|
Application DetailsProduct Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Handling References for anti-IL4 Receptor Antibody (ABIN2688938) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Application Details References for anti-IL4 Receptor Antibody (ABIN2688938) Images back to top
|Buffer||Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.|
|Storage Comment||Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
References for anti-IL4 Receptor Antibody (ABIN2688938)Product Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Application Details Handling Images back to top
|Product cited in:||
Hassuneh, Nagarkatti, Nagarkatti: "Evidence for the participation of interleukin-2 (IL-2) and IL-4 in the regulation of autonomous growth and tumorigenesis of transformed cells of lymphoid origin." in: Blood, Vol. 89, Issue 2, pp. 610-20, 1997 (PubMed).
Chilton, Fernandez-Botran: "Production of soluble IL-4 receptors by murine spleen cells is regulated by T cell activation and IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 5907-17, 1994 (PubMed).
Sempowski, Beckmann, Derdak et al.: "Subsets of murine lung fibroblasts express membrane-bound and soluble IL-4 receptors. Role of IL-4 in enhancing fibroblast proliferation and collagen synthesis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3606-14, 1994 (PubMed).
Feldman, Ruhl, Bickel et al.: "Regulation of interleukin-4 receptors on murine myeloid progenitor cells by interleukin-6." in: Blood, Vol. 78, Issue 7, pp. 1678-84, 1991 (PubMed).