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anti-Human IL5 antibody for ELISA (Detection)

Recommended IL5 Antibody (supplied by: Log in to see )

Antigen
Interleukin 5 (IL5) Antibodies
  • EDF
  • IL-5
  • Il-5
  • IL5
  • Pro-TRH
  • TRF
Reactivity
Human
165
129
19
16
16
2
1
1
1
1
Host
Rat
126
94
19
2
Clonality
Monoclonal
Conjugate
This IL5 antibody is un-conjugated
42
12
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
ELISA (Capture), ELISA (Detection), Enzyme Immunoassay (EIA)
123
119
91
34
16
14
11
11
10
9
9
8
8
6
5
4
4
3
3
1
1
Supplier
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Catalog No. ABIN371620
$ 335.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000875046 ABIN371619 ELISA (Capture) ELISA (Detection) EIA Biotin Rat IgG2a Log in to see JES1-5A10
0.000875046 ABIN279975 ELISA (Detection) ELISPOT (Detection) Biotin Rat IgG2a kappa Log in to see JES1-5A10 1

Top referenced anti-IL5 antibody for ELISA (Detection)

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Immunoprecipitation (IP) 4 Antibodies

General

Antigen Interleukin 5 (IL5) Antibodies
Reactivity Human
(165), (129), (19), (16), (16), (2), (1), (1), (1), (1)
Host Rat
(126), (94), (19), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL5 antibody is un-conjugated
(42), (12), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application ELISA (Capture), ELISA (Detection), Enzyme Immunoassay (EIA)
(123), (119), (91), (34), (16), (14), (11), (11), (10), (9), (9), (8), (8), (6), (5), (4), (4), (3), (3), (1), (1)
Pubmed 3 references available
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Product Details anti-IL5 Antibody

Target Details IL5 Application Details Handling References for anti-IL5 Antibody (ABIN371620) Images
Specificity This antibody is specific to Human Interleukin-5/IL-5. (Ref.1-4)
Characteristics Synonyms: IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor,TRF
Purification Purified
Immunogen COS-expressed recombinant human IL-5.
Clone JES1-5A10
Isotype IgG2a

Target Details IL5

Product Details anti-IL5 Antibody Application Details Handling References for anti-IL5 Antibody (ABIN371620) Images back to top
Antigen
Alternative Name Interleukin-5 / IL5 (IL5 Antibody Abstract)
Background Human Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide-linked homodimeric glycoprotein with 115 amino-acid residues in each chain. Known also as EDG (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two-domain structure, with each domain showing significant homology to the cytokine fold in GM-CSF, M-CSF, IL2, IL4 and growth hormone. Human and Mouse IL5 have 70 % amino acid sequence homology.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF
Gene ID 3567
UniProt P05113
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL5 Antibody Target Details IL5 Handling References for anti-IL5 Antibody (ABIN371620) Images back to top
Application Notes ELISA: Useful useful as a Detection/Capture antibody in a Sandwich ELISA for quantifyinghuman IL-5 protein levels. (Ref.1-5)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL5 Antibody Target Details IL5 Application Details References for anti-IL5 Antibody (ABIN371620) Images back to top
Concentration 0.5 mg/mL
Buffer 100 mM Borate buffered saline, pH 8.2 without preservatives or amine-containing buffer salts.
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-IL5 Antibody (ABIN371620)

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling Images back to top
Background publications

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Butterfield, Leiferman, Abrams, Silver, Bower, Gonchoroff, Gleich: "Elevated serum levels of interleukin-5 in patients with the syndrome of episodic angioedema and eosinophilia." in: Blood, Vol. 79, Issue 3, pp. 688-92, 1992

Limaye, Abrams, Silver, Awadzi, Francis, Ottesen, Nutman: "Interleukin-5 and the posttreatment eosinophilia in patients with onchocerciasis." in: The Journal of clinical investigation, Vol. 88, Issue 4, pp. 1418-21, 1991