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|Antigen||Interleukin 5 (IL5) Antibodies|
|Conjugate||This IL5 antibody is un-conjugated Alternatives|
ELISA (Capture), ELISA (Detection), Enzyme Immunoassay (EIA)
|3 references available|
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Product Details anti-IL5 AntibodyTarget Details IL5 Application Details Handling References for anti-IL5 antibody (ABIN371620) Images
|Specificity||This antibody is specific to Human Interleukin-5/IL-5. (Ref.1-4)|
|Characteristics||Synonyms: IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor,TRF|
|Immunogen||COS-expressed recombinant human IL-5.|
Target Details IL5Product Details anti-IL5 Antibody Application Details Handling References for anti-IL5 antibody (ABIN371620) Images back to top
|Alternative Name||Interleukin-5 / IL5 (IL5 Antibody Abstract)|
|Background||Human Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide-linked homodimeric glycoprotein with 115 amino-acid residues in each chain. Known also as EDG (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two-domain structure, with each domain showing significant homology to the cytokine fold in GM-CSF, M-CSF, IL2, IL4 and growth hormone. Human and Mouse IL5 have 70 % amino acid sequence homology.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF|
|Pathways||JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour|
Application DetailsProduct Details anti-IL5 Antibody Target Details IL5 Handling References for anti-IL5 antibody (ABIN371620) Images back to top
ELISA: Useful useful as a Detection/Capture antibody in a Sandwich ELISA for quantifyinghuman IL-5 protein levels. (Ref.1-5)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL5 Antibody Target Details IL5 Application Details References for anti-IL5 antibody (ABIN371620) Images back to top
|Buffer||100 mM Borate buffered saline, pH 8.2 without preservatives or amine-containing buffer salts.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-IL5 antibody (ABIN371620)Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling Images back to top
Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992
Butterfield, Leiferman, Abrams, Silver, Bower, Gonchoroff, Gleich: "Elevated serum levels of interleukin-5 in patients with the syndrome of episodic angioedema and eosinophilia." in: Blood, Vol. 79, Issue 3, pp. 688-92, 1992
Limaye, Abrams, Silver, Awadzi, Francis, Ottesen, Nutman: "Interleukin-5 and the posttreatment eosinophilia in patients with onchocerciasis." in: The Journal of clinical investigation, Vol. 88, Issue 4, pp. 1418-21, 1991