anti-Mouse (Murine) IL5 antibody for ELISA

Recommended IL5 Antibody (supplied by: Log in to see )

Antigen
Interleukin 5 (IL5) Antibodies
  • IL5
  • Il-5
  • IL-5
  • TRF
  • EDF
  • Pro-TRH
  • interleukin 5
  • Interleukin 5
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5-like
  • thyrotropin-releasing hormone
  • IL5
  • Il5
  • IL-5
  • LOC100358075
  • TRH
Reactivity
Mouse (Murine)
211
147
27
20
17
3
2
2
1
1
1
Host
Rat
132
103
41
2
Clonality
Monoclonal
Conjugate
This IL5 antibody is un-conjugated
39
15
9
9
8
8
7
7
7
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Intracellular Staining (ICS), Neutralization (Neut), ELISA
126
126
124
24
23
13
11
10
10
9
9
8
7
6
6
6
5
4
2
2
1
1
Options
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Catalog No. ABIN1176999
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3185179 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1453494 IHC ELISA WB Rabbit IgG AA 43-92 Log in to see Polyclonal
1 ABIN2475029 FACS ELISA WB Rat IgG1 Log in to see TRFK5 4
1 ABIN2689774 ELISA Biotin Rat IgG2a Log in to see TRFK4 4
1 ABIN2658791 Func FACS IP ELISA APC Rat IgG1 kappa Log in to see TRFK5 9
1 ABIN2658792 Func FACS IP ELISA APC Rat IgG1 kappa Log in to see TRFK5 9
1 ABIN254410 FACS ELISA WB Rat IgG1 Log in to see TRFK5
1 ABIN2689772 Inhibition ELISA WB Rat IgG1, kappa Log in to see TRFK5 8
1 ABIN208744 ELISA Biotin Rat IgG2a Log in to see
1 ABIN218125 FACS ELISA WB Rat IgG1 Log in to see
1 ABIN218121 ELISA Biotin Rat IgG2a, kappa Log in to see
1 ABIN233214 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN4325343 ELISA FACS WB Alexa Fluor 405 Rat IgG1 Log in to see TRFK5
1 ABIN4325345 ELISA FACS WB Alexa Fluor 647 Rat IgG1 Log in to see TRFK5
1 ABIN4325346 ELISA FACS WB Alexa Fluor 700 Rat IgG1 Log in to see TRFK5
1 ABIN4325344 ELISA FACS WB Alexa Fluor 488 Rat IgG1 Log in to see TRFK5
1 ABIN4325325 ELISA FACS WB DyLight 488 Rat IgG1 Log in to see TRFK5
1 ABIN4325320 ELISA FACS WB DyLight 350 Rat IgG1 Log in to see TRFK5
1 ABIN4325321 ELISA FACS WB DyLight 405 Rat IgG1 Log in to see TRFK5
1 ABIN4325322 ELISA FACS WB PerCP Rat IgG1 Log in to see TRFK5

General

Antigen Interleukin 5 (IL5) Antibodies
Reactivity Mouse (Murine)
(211), (147), (27), (20), (17), (3), (2), (2), (1), (1), (1)
Host Rat
(132), (103), (41), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL5 antibody is un-conjugated
(39), (15), (9), (9), (8), (8), (7), (7), (7), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS), Neutralization (Neut), ELISA
(126), (126), (124), (24), (23), (13), (11), (10), (10), (9), (9), (8), (7), (6), (6), (6), (5), (4), (2), (2), (1), (1)
Pubmed 9 references available
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Product Details anti-IL5 Antibody

Target Details IL5 Application Details Handling References for anti-IL5 antibody (ABIN1176999) Images
Brand BD Pharmingen™
Characteristics Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IL-5. Neutralization activity may be measured with a proliferation assay using 1.5 ng/mL recombinant human IL-5 (Cat. No. 554606) to stimulate TF-1 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant human IL-5 for 60 minutes).
50% Neutralization (ND50) at 5 - 50 ng/mL
>95% Neutralization at 50 - 100 ng/mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Mouse Semi-Purified T-Cell
Clone TRFK5
Isotype IgG1, kappa

Target Details IL5

Product Details anti-IL5 Antibody Application Details Handling References for anti-IL5 antibody (ABIN1176999) Images back to top
Antigen
Alternative Name IL-5 (IL5 Antibody Abstract)
Background The TRFK5 antibody reacts with mouse interleukin-5 (IL-5) and cross-reacts with human IL-5. The TRFK5 antibody has been reported to cross react with IL-5 from rhesus monkey. This is a neutralizing antibody.
Research Area Cancer
Pathways JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour

Application Details

Product Details anti-IL5 Antibody Target Details IL5 Handling References for anti-IL5 antibody (ABIN1176999) Images back to top
Application Notes ELISA: The purified format of the TRFK5 antibody (Cat. No.554393) has been reported to be useful as a capture antibody for sandwich ELISA for measuring mouse or human IL-5. For a mouse IL-5 ELISA, purified TRFK5 antibody can be paired with the biotinylated TRFK4 antibody (Cat. No. 554397) as the detection antibody, with recombinant mouse IL-5 (Cat. No. 554581) as the standard. Alternatively, investigators may be interested in the BD OptEIA™ Mouse IL-5 ELISA Set (Cat. No. 555236). For a human IL-5 ELISA, purified TRFK5 antibody can be paired with the biotinylated JES1-5A10 antibody (Cat. No. 554491) as the detecting antibody, with recombinant human IL-5 (Cat. No. 554606) as the standard. Alternatively, investigators may be interested in the BD OptEIA™ Human IL-5 ELISA Set (Cat. No 555202).
Restrictions For Research Use only

Handling

Product Details anti-IL5 Antibody Target Details IL5 Application Details References for anti-IL5 antibody (ABIN1176999) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL5 antibody (ABIN1176999)

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling Images back to top
Product cited in:

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, 2008

Mo, Sarawar, Doherty: "Induction of cytokines in mice with parainfluenza pneumonia." in: Journal of virology, Vol. 69, Issue 2, pp. 1288-91, 1995

Litton, Sander, Murphy, OGarra, Abrams: "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." in: Journal of immunological methods, Vol. 175, Issue 1, pp. 47-58, 1994

Sarawar, Sangster, Coffman, Doherty: "Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 1246-53, 1994

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Kitamura, Takaku, Miyajima: "IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoietic cell line, TF-1." in: International immunology, Vol. 3, Issue 6, pp. 571-7, 1991

Sander, Andersson, Andersson: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." in: Immunological reviews, Vol. 119, pp. 65-93, 1991

Swain, Weinberg, English, Huston: "IL-4 directs the development of Th2-like helper effectors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3796-806, 1991

Schumacher, OGarra, Shrader, van Kimmenade, Bond, Mosmann, Coffman: "The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 5, pp. 1576-81, 1988