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anti-Mouse (Murine) IL5 antibody for ELISpot

Recommended IL5 Antibody (supplied by: Log in to see )

Antigen
Interleukin 5 (IL5) Antibodies
  • EDF
  • IL-5
  • Il-5
  • IL5
  • Pro-TRH
  • TRF
Reactivity
Human, Mouse (Murine)
136
92
19
3
3
2
1
1
1
1
Host
Rat
92
90
12
2
Clonality
Monoclonal
Conjugate
This IL5 antibody is un-conjugated
42
10
4
3
3
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA (Capture), ELISpot, Immunohistochemistry (IHC), Neutralization (Neut)
110
102
50
19
15
11
11
10
9
9
9
7
6
5
4
4
4
3
1
1
Supplier
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Catalog No. ABIN282511
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008755788 ABIN279973 ELISA (Detection) ELISpot Biotin Rat IgG2a kappa Log in to see TRFK4 1
0.0008755788 ABIN1177354 ELISpot Log in to see

Top referenced anti-IL5 antibody for ELISpot

Similar anti-IL5 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 2 Antibodies
ELISA 46 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 3 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 30 Antibodies
Functional Studies (Func) 4 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Intracellular Staining (ICS) 6 Antibodies

General

Antigen Interleukin 5 (IL5) Antibodies
Reactivity Human, Mouse (Murine)
(136), (92), (19), (3), (3), (2), (1), (1), (1), (1)
Host Rat
(92), (90), (12), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL5 antibody is un-conjugated
(42), (10), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA (Capture), ELISpot, Immunohistochemistry (IHC), Neutralization (Neut)
(110), (102), (50), (19), (15), (11), (11), (10), (9), (9), (9), (7), (6), (5), (4), (4), (4), (3), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL5 Antibody

Target Details IL5 Application Details Handling References for anti-IL5 Antibody (ABIN282511) Images
Specificity The TRFK5 antibody reacts with mouse and human interleukin-5 (IL-5). The TRFK5 antibody is a neutralizing antibody. Mouse IL-5 is a disulfide-linked homodimer, containing two 113 amino acid peptides, these resolve as 32-34 kDa bands in SDS-PAGE. IL-5 is produced by T cells and has been known as eosinophil-differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF). IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation. In mice, IL-5 has been shown to stimulate B cell proliferation and antibody production.
Characteristics The TRFK5 antibody reacts with mouse and human interleukin-5 (IL-5). The TRFK5 antibody is a neutralizing antibody. Mouse IL-5 is a disulfide- linked homodimer, containing two 113 amino acid peptides, these resolve as 32-34 kDa bands in SDS-PAGE. IL-5 is produced by T cells and has been known as eosinophil-differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF). IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation. In mice, IL-5 has been shown to stimulate B cell proliferation and antibody production.
Synonyms: Synonyms: Interleukin-5, IL5
Purification Affinity Purified
Endotoxin Level <0.001 ng endotoxin/μg
Grade Functional Grade
Clone TRFK5
Isotype IgG1 kappa

Target Details IL5

Product Details anti-IL5 Antibody Application Details Handling References for anti-IL5 Antibody (ABIN282511) Images back to top
Antigen
Alternative Name IL-5 (IL5 Antibody Abstract)
Background The TRFK5 antibody is a neutralizing antibody. Mouse IL-5 is a disulfide-linked homodimer, containing two 113 amino acid peptides, these resolve as 32-34 kDa bands in SDS-PAGE. IL-5 is produced by T cells and has been known as eosinophil-differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF). IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation. In mice, IL-5 has been shown to stimulate B cell proliferation and antibody production.
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL5 Antibody Target Details IL5 Handling References for anti-IL5 Antibody (ABIN282511) Images back to top
Application Notes Applications reported: The TRFK5 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-5 bioactivity, and IHC.

Applications tested: The Functional Grade Purified TRFK5 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-5 bioactivity. The TRFK5 antibody at 0.06 μg/mL has been found to inhibit by 50 % the biological effects of 1.0 ng/mL mouse IL-5 in an MC/9 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section. The TRFK5 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IL-5 in combination with the biotin TRFK4 (13-7051) antibody for detection and recombinant mouse IL-5 (14-8051) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. The TRFK5 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-5 in combination with the biotinylated JES1-5A10 (13-7059) antibody for detection and recombinant human IL-5 (14-80591) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.
Restrictions For Research Use only

Handling

Product Details anti-IL5 Antibody Target Details IL5 Application Details References for anti-IL5 Antibody (ABIN282511) Images back to top
Concentration 1 mg/mL
Buffer aqueous buffer, no sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Use in sterile environment.
Storage 4 °C

References for anti-IL5 Antibody (ABIN282511)

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling Images back to top
Background publications

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Images

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Supplier Images
 image for anti-IL5 antibody (Interleukin 5) (ABIN282511) Naive mouse splenocytes (SJL/J strain) were stimulated with 2 ug/ml ConA in HL-1 medi...
 image for anti-IL5 antibody (Interleukin 5) (ABIN282511) anti-Interleukin 5 (IL5) antibody (Image 2)