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anti-Mouse (Murine) IL5 antibody for Flow Cytometry

Recommended IL5 Antibody (supplied by: Log in to see )

Antigen
Interleukin 5 (IL5) Antibodies
  • EDF
  • IL-5
  • Il-5
  • IL5
  • Pro-TRH
  • TRF
Reactivity
Guinea Pig, Human, Mouse (Murine)
165
128
19
16
15
2
1
1
1
1
Host
Rat
126
94
19
2
Clonality
Monoclonal
Conjugate
This IL5 antibody is un-conjugated
42
12
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
123
118
90
34
16
14
11
11
10
9
9
8
8
6
5
4
4
4
4
1
1
Supplier
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Catalog No. ABIN118519
$ 731.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010463598 ABIN281625 ELISA (Capture) ELISPOT (Capture) FACS ICS Neut Rat IgG1 kappa Log in to see TRFK5 1
0.0010463598 ABIN2661236 FACS Biotin Rat IgG2a, IgG2a kappa Log in to see TRFK4 8
0.0010463598 ABIN2665842 FACS Rat IgG1 kappa Log in to see TRFK5 9
0.0010463598 ABIN2665178 Func FACS Rat IgG1 kappa Log in to see TRFK5 9
0.0010463598 ABIN218125 FACS ELISA WB Rat IgG1 Log in to see
0.0010463598 ABIN2658791 Func FACS IP ELISA APC Rat IgG1 kappa Log in to see TRFK5 9
0.0010463598 ABIN2658792 Func FACS IP ELISA APC Rat IgG1 kappa Log in to see TRFK5 9
0.0010463598 ABIN2663799 FACS PE Rat IgG1 kappa Log in to see TRFK5 9
0.0010463598 ABIN2663798 FACS PE Rat IgG1 kappa Log in to see TRFK5 9
0.0010463598 ABIN2661235 FACS Biotin Rat IgG2a, IgG2a kappa Log in to see TRFK4 8
0.0010463598 ABIN2665841 FACS Rat IgG1 kappa Log in to see TRFK5 9
0.0010463598 ABIN217640 FACS ELISA WB Rat IgG1 Log in to see TRFK5
0.0010463598 ABIN2665177 Func FACS Rat IgG1 kappa Log in to see TRFK5 9
0.0010463598 ABIN254410 FACS ELISA WB Rat IgG1 Log in to see TRFK5
0.0010463598 ABIN218122 FACS IF ELISA WB Rat IgG1, kappa Log in to see
0.0010463598 ABIN218123 FACS IF Neut ELISA WB Rat IgG1, kappa Log in to see
0.0010463598 ABIN279106 FACS ICS PE Rat IgG1 kappa Log in to see TRFK5 1
0.0010463598 ABIN2475029 FACS ELISA WB Rat IgG1 Log in to see TRFK5 4
0.0010463598 ABIN957686 FACS IF ELISA WB Rat IgG1, kappa Log in to see TRFK5
0.0010463598 ABIN4325329 FACS Alexa Fluor 488 Rat IgG1 Log in to see TRFK5

Top referenced anti-IL5 antibody for Flow Cytometry

Similar anti-IL5 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 2 Antibodies
ELISA 59 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 3 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 64 Antibodies
Functional Studies (Func) 4 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Intracellular Staining (ICS) 6 Antibodies

General

Antigen Interleukin 5 (IL5) Antibodies
Reactivity Guinea Pig, Human, Mouse (Murine)
(165), (128), (19), (16), (15), (2), (1), (1), (1), (1)
Host Rat
(126), (94), (19), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL5 antibody is un-conjugated
(42), (12), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
(123), (118), (90), (34), (16), (14), (11), (11), (10), (9), (9), (8), (8), (6), (5), (4), (4), (4), (4), (1), (1)
Pubmed 4 references available
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Product Details anti-IL5 Antibody

Target Details IL5 Application Details Handling References for anti-IL5 Antibody (ABIN118519) Images
Specificity This antibody is specific for Interleukin 5 (IL-5). Clone TRFK5 may be used to neutralise IL-5 bioactivity in a functional assay (1,2).
Purification Affinity chromatography on Protein G
Immunogen Purified natural murine IL-5.
Clone TRFK5
Isotype IgG1

Target Details IL5

Product Details anti-IL5 Antibody Application Details Handling References for anti-IL5 Antibody (ABIN118519) Images back to top
Antigen
Alternative Name Interleukin-5 / IL5 (IL5 Antibody Abstract)
Background IL-5 is a T cell derived haematopoietic growth factor, which stimulates eosinophil differentiation and activation.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF
Gene ID 3567
NCBI Accession NP_000870
UniProt P05113
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL5 Antibody Target Details IL5 Handling References for anti-IL5 Antibody (ABIN118519) Images back to top
Application Notes Flow Cytometry: Membrane permeabilisation is required for this application. ELISA: This antibody may be used in combination with clone TRFK4 (ABIN181863) in ELISA and ELISPOT assays. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL5 Antibody Target Details IL5 Application Details References for anti-IL5 Antibody (ABIN118519) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-IL5 Antibody (ABIN118519)

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling Images back to top
Background publications

Akdis, Akdis, Blesken, Wymann, Alkan, Mueller, Blaser: "Epitope-specific T cell tolerance to phospholipase A2 in bee venom immunotherapy and recovery by IL-2 and IL-15 in vitro." in: The Journal of clinical investigation, Vol. 98, Issue 7, pp. 1676-83, 1996

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Betz, Fox: "Regulation and development of cytochrome c-specific IL-4-producing T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 4, pp. 1046-52, 1990

Schumacher, OGarra, Shrader, van Kimmenade, Bond, Mosmann, Coffman: "The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 5, pp. 1576-81, 1988