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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||75 Antibodies||40 Antibodies||9 Antibodies|
|Neutralization (Neut)||13 Antibodies||9 Antibodies|
|Intracellular Staining (ICS)||6 Antibodies||4 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||8 Antibodies||5 Antibodies||5 Antibodies|
|Immunohistochemistry (IHC)||16 Antibodies||5 Antibodies||4 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Antigen||Interleukin 5 (IL5) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This IL5 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-IL5 AntibodyTarget Details IL5 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of IL-5 protein. (region surrounding Ser92)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details IL5Product Details anti-IL5 Antibody Application Details Handling Images back to top
|Alternative Name||Interleukin-5 / IL5 (IL5 Antibody Abstract)|
|Background||Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cellreplacing factor (TRF), that induces B cell and eosinophil growth and differentiation. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of two chains: an alpha chain that binds IL-5 with low affinity and a beta chain that does not bind IL-5, but together with the IL-5 alpha chain, constitutes the high affinity IL-5 receptor. The cytoplasmic domain of both the alpha and beta chains is essential for signal transduction. Specifically, the membraneproximal proline-rich sequence of the cytoplasmic domain of the IL-5R receptor alpha subunit, IL-5Ralpha, is critical for the IL-5 induced proliferative response, expression of nuclear protooncogenes and tyrosine phosphorylation of cellular proteins, such as JAK1 and JAK2. Alternative splicing of the IL-5Ralpha gene produces several isoforms, including a membraneanchored isoform and a soluble isoform. The soluble isoform competes with IL-5 for binding to IL-5R and inhibits IL-5-mediated receptor activation and inflammatory mediator production, and, therefore, may be useful in treating asthma.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF|
|Molecular Weight||approx. 45 kDa|
Application DetailsProduct Details anti-IL5 Antibody Target Details IL5 Handling Images back to top
Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL5 Antibody Target Details IL5 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-IL5 Antibody Target Details IL5 Application Details Handling back to top