Human IL5 ELISA Kit

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Antigen
Interleukin 5 (IL5) ELISA Kits
  • IL5
  • Il-5
  • IL-5
  • TRF
  • EDF
  • Pro-TRH
  • interleukin 5
  • Interleukin 5
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5-like
  • thyrotropin-releasing hormone
  • IL5
  • Il5
  • IL-5
  • LOC100358075
  • TRH
Epitope
AA 20-134
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
63
38
14
9
8
7
7
6
6
5
5
4
2
2
1
1
Method Type
Sandwich ELISA
Detection Range
7.8-500 pg/mL
Minimum Detection Limit
7.8 pg/mL
Application
ELISA
Options
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Catalog No. ABIN921063
$ 320.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.701447 ABIN2472121 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 7.81-500 pg/mL 7.81 pg/mL Log in to see
9.701447 ABIN414885 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
9.701447 ABIN365168 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.78-50 pg/mL 0.78 pg/mL Log in to see
9.701447 ABIN1379946 Sandwich ELISA Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
9.701447 ABIN625025 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 5-2000 pg/mL 5 pg/mL Log in to see
9.701447 ABIN2708290 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 3.9-250 pg/mL 3.9 pg/mL Log in to see
9.701447 ABIN2708291 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
9.701447 ABIN5026941 Sandwich ELISA Plasma, Serum, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
9.701447 ABIN2114329 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin), Urine Log in to see
9.701447 ABIN2064526 Cell Culture Supernatant, Serum, Plasma Log in to see
1 ABIN1029018 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN4993140 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 2.7-2000 pg/mL 2.7 pg/mL Log in to see
1 ABIN509707 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
1 ABIN5592266 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN1115670 Sandwich ELISA Serum, Plasma, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN2684708 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see 3
1 ABIN455596 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN5520982 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN1112651 Sandwich ELISA Serum, Body Fluids, Tissue Lysate, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN1446057 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Samples 5pg/mL Log in to see

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General

Antigen Interleukin 5 (IL5) ELISA Kits
Epitope AA 20-134
Reactivity Human
Kits with alternative reactivity to:
(63), (38), (14), (9), (8), (7), (7), (6), (6), (5), (5), (4), (2), (2), (1), (1)
Method Type Sandwich ELISA
Detection Range 7.8-500 pg/mL
Minimum Detection Limit 7.8 pg/mL
Application ELISA
Pubmed 6 references available
Supplier Log in to see

Product Details IL5 ELISA Kit

Target details Application Details Handling ProductDetails: References for IL5 Kit (ABIN921063) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-5
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: sf21
Immunogen sequence: I20-S134
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <2pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: I20-S134

Target details

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Antigen
Alternative Name IL5 (IL5 ELISA Kit Abstract)
Background

Protein Function: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells.

Background: Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70 % amino acid sequence homology.

Synonyms: Interleukin-5,IL-5,B-cell differentiation factor I,Eosinophil differentiation factor,T-cell replacing factor,TRF,IL5,

Full Gene Name: Interleukin-5

Cellular Localisation: Secreted.
Gene ID 3567
UniProt P05113
Research Area Cancer, Cytokines, Innate Immunity, Adaptive Immunity
Pathways JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour

Application Details

Product Details IL5 ELISA Kit Target details Handling ProductDetails: References for IL5 Kit (ABIN921063) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Sequence similarities: Belongs to the IL-5 family.

Plate Pre-coated
Protocol human IL-5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-5 has been precoated onto 96-well plates. Standards(sf21, I20-S134) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-5 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human IL-5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-5 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 44.6, Standard deviation: 2.32, CV(%): 5.2
  • Sample 2: n=16, Mean(pg/ml): 168, Standard deviation: 6.89, CV(%): 4.1
  • Sample 3: n=16, Mean(pg/ml): 338, Standard deviation: 14.87, CV(%): 4.4,
  • Sample 1: n=24, Mean(pg/ml): 43.8, Standard deviation: 2.63, CV(%): 6
  • Sample 2: n=24, Mean(pg/ml): 188, Standard deviation: 10.34, CV(%): 5.5
  • Sample 3: n=24, Mean(pg/ml): 329, Standard deviation: 21.06, CV(%): 6.4
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

ProductDetails: References for IL5 Kit (ABIN921063)

Product Details IL5 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, 2015

Wang, Deng, Ren, Xiao, Chen, Tao: "Lactoferrin administration into the nostril alleviates murine allergic rhinitis and its mechanisms." in: Scandinavian journal of immunology, Vol. 78, Issue 6, pp. 507-15, 2013

Chen, Duan, Kang, Yang, Jiang, Zhang, Li, Yin, Hu, Dong, Li, Hajjar, Han: "Activation of liver X receptor induces macrophage interleukin-5 expression." in: The Journal of biological chemistry, Vol. 287, Issue 52, pp. 43340-50, 2012

Zhu, Qiu, Liu, Dong, Weng, Hu, Kuang, Gao, Dan, Tao: "Tolerogenic dendritic cells generated by RelB silencing using shRNA prevent acute rejection." in: Cellular immunology, Vol. 274, Issue 1-2, pp. 12-8, 2012

Tong, Bai, Liu, Wang, Zhang, Xiao, Xu, Liu, Yang, Wu: "Expression, purification and monoclonal antibodies preparation of recombinant equine mature interleukin-18." in: Veterinary immunology and immunopathology, Vol. 136, Issue 3-4, pp. 194-200, 2010

Background publications

Le Beau, Lemons, Espinosa, Larson, Arai, Rowley: "Interleukin-4 and interleukin-5 map to human chromosome 5 in a region encoding growth factors and receptors and are deleted in myeloid leukemias with a del(5q)." in: Blood, Vol. 73, Issue 3, pp. 647-50, 1989

Images

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Supplier Images
ELISA image for IL5 ELISA Kit (Interleukin 5) (ABIN921063) Human IL-5 PicoKine ELISA Kit standard curve