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|Application / Reactivity||Human|
|ELISA||44 ELISA Kits|
|Antigen||Interleukin 5 (IL5) ELISA Kits|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||7.8-500 pg/mL|
|Minimum Detection Limit||7.8 pg/mL|
|5 references available|
|Supplier||Log in to see|
Product Details IL5 ELISA KitTarget details Application Details Handling References for IL5 Kit (ABIN921063) Images
|Purpose||For quantitative detection of human IL-5 in cell culture supernates, serum, plasma (heparin, EDTA) and urine.|
|Sample Type||Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine|
|Specificity||Natural and recombinant human IL-5|
|Cross-Reactivity (Details)||There is no detectable cross-reactivity with other relevant proteins.|
|Material not included||Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl|
Expression system for standard: sf21
Immunogen sequence: I20-S134
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|Background||Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70 % amino acid sequence homology.|
|Research Area||Cancer, Cytokines, Innate Immunity, Adaptive Immunity|
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|Application Notes||Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.|
|Plate||Pre-coated,Strips (12 x 8)|
|Protocol||human IL-5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-5 has been precoated onto 96-well plates. Standards(sf21, I20-S134) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-5 amount of sample captured in plate.|
|Assay Procedure||Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human IL-5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-5 standard solution and each sample be measured in duplicate.|
|Restrictions||For Research Use only|
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|Storage||-20 °C,4 °C|
|Storage Comment||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles|
|Expiry Date||12 months|
References for IL5 Kit (ABIN921063)Product Details IL5 ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Presti, DOrazio, Labra et al.: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, 2015 (PubMed).
Wang, Deng, Ren et al.: "Lactoferrin administration into the nostril alleviates murine allergic rhinitis and its mechanisms." in: Scandinavian journal of immunology, Vol. 78, Issue 6, pp. 507-15, 2013 (PubMed).
Chen, Duan, Kang et al.: "Activation of liver X receptor induces macrophage interleukin-5 expression." in: The Journal of biological chemistry, Vol. 287, Issue 52, pp. 43340-50, 2012 (PubMed).
Tong, Bai, Liu et al.: "Expression, purification and monoclonal antibodies preparation of recombinant equine mature interleukin-18." in: Veterinary immunology and immunopathology, Vol. 136, Issue 3-4, pp. 194-200, 2010 (PubMed).
Le Beau, Lemons, Espinosa et al.: "Interleukin-4 and interleukin-5 map to human chromosome 5 in a region encoding growth factors and receptors and are deleted in myeloid leukemias with a del(5q)." in: Blood, Vol. 73, Issue 3, pp. 647-50, 1989 (PubMed).
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