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Human IL5 ELISA Kit

Recommended IL5 Product (supplied by: Log in to see )

Antigen
Interleukin 5 (IL5) ELISA Kits
  • EDF
  • IL-5
  • Il-5
  • IL5
  • Pro-TRH
  • TRF
Epitope
AA 20-134
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
62
39
12
9
7
7
6
6
4
4
4
3
2
2
Methode Type
Sandwich ELISA
Detection Range
7.8-500 pg/mL
Minimum Detection Limit
7.8 pg/mL
Application
ELISA
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Catalog No. ABIN921063
$ 320.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32682037 ABIN2472121 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.32682037 ABIN414885 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.32682037 ABIN1379946 Sandwich ELISA Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
0.32682037 ABIN2708290 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 3.9-250 pg/mL 3.9 pg/mL Log in to see
0.32682037 ABIN2708291 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.32682037 ABIN2064526 Cell Culture Supernatant, Serum, Plasma Log in to see
0.32682037 ABIN2762289 Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate), Plasma (heparin) 3.9-250 pg/mL 3.9 pg/mL Log in to see
0.32682037 ABIN2114329 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin), Urine Log in to see
0.32682037 ABIN1033566 Sandwich ELISA Log in to see
0.0012100277 ABIN365168 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.78-50 pg/mL 0.78 pg/mL Log in to see
0.0012100277 ABIN509707 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012100277 ABIN1115670 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012100277 ABIN2684708 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see 3
0.0012100277 ABIN1029018 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012100277 ABIN1112651 Sandwich ELISA Serum, Body Fluids, Tissue Lysate, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012100277 ABIN1446057 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Samples 5pg/mL Log in to see
0.0012100277 ABIN455596 Cell Culture Supernatant, Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012100277 ABIN625025 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine 5-2000 pg/mL 5 pg/mL Log in to see
0.0012100277 ABIN2748871 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 3.9-250 pg/mL 3.9 pg/mL Log in to see
0.0012100277 ABIN2114327 Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see

Top referenced IL5 ELISA Kit for ELISA

  • Human IL5 ELISA Kit for Sandwich ELISA - ABIN921063 (5 Publications): Le Beau, Lemons, Espinosa, Larson, Arai, Rowley: Interleukin-4 and interleukin-5 map to human chromosome 5 in a region encoding growth factors and receptors and are deleted in myeloid leukemias with a del(5q). in Blood 1989 (PubMed)

  • Human IL5 ELISA Kit - ABIN1305305 (1 Publications): Curti, Ratta, Corinti, Girolomoni, Ricci, Tazzari, Siena, Grande, Fogli, Tura, Lemoli: Interleukin-11 induces Th2 polarization of human CD4(+) T cells. in Blood 2001 (PubMed)

Similar IL5 ELISA Kits

Application / Reactivity Human
ELISA 44 ELISA Kits

General

Antigen Interleukin 5 (IL5) ELISA Kits
Epitope AA 20-134
Reactivity Human
Kits with alternative reactivity to:
(62), (39), (12), (9), (7), (7), (6), (6), (4), (4), (4), (3), (2), (2)
Methode Type Sandwich ELISA
Detection Range 7.8-500 pg/mL
Minimum Detection Limit 7.8 pg/mL
Application ELISA
Pubmed 5 references available
Supplier Log in to see

Product Details IL5 ELISA Kit

Target details Application Details Handling References for IL5 Kit (ABIN921063) Images
Purpose For quantitative detection of human IL-5 in cell culture supernates, serum, plasma (heparin, EDTA) and urine.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
Detection Method Colorimetric
Specificity Natural and recombinant human IL-5
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <2pg/mL
Components
  • 96-well plate precoated with anti- human IL-5 antibod - 1
  • Lyophilized recombinant human IL-5 standar - 10ng/tubex2
  • Biotinylated anti- human IL-5 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: I20-S134

Target details

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Antigen
Alternative Name IL5 (IL5 ELISA Kit Abstract)
Background Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70 % amino acid sequence homology.
Gene ID 3567
UniProt P05113
Research Area Cancer, Cytokines, Innate Immunity, Adaptive Immunity
Pathways JAK-STAT Signaling

Application Details

Product Details IL5 ELISA Kit Target details Handling References for IL5 Kit (ABIN921063) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human IL-5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-5 has been precoated onto 96-well plates. Standards(sf21, I20-S134) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-5 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human IL-5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-5 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 44.6, Standard deviation: 2.32, CV(%): 5.2
  • Sample 2: n=16, Mean(pg/ml): 168, Standard deviation: 6.89, CV(%): 4.1
  • Sample 3: n=16, Mean(pg/ml): 338, Standard deviation: 14.87, CV(%): 4.4,
  • Sample 1: n=24, Mean(pg/ml): 43.8, Standard deviation: 2.63, CV(%): 6
  • Sample 2: n=24, Mean(pg/ml): 188, Standard deviation: 10.34, CV(%): 5.5
  • Sample 3: n=24, Mean(pg/ml): 329, Standard deviation: 21.06, CV(%): 6.4
Restrictions For Research Use only

Handling

Product Details IL5 ELISA Kit Target details Application Details References for IL5 Kit (ABIN921063) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL5 Kit (ABIN921063)

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Product cited in:

Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, 2015

Wang, Deng, Ren, Xiao, Chen, Tao: "Lactoferrin administration into the nostril alleviates murine allergic rhinitis and its mechanisms." in: Scandinavian journal of immunology, Vol. 78, Issue 6, pp. 507-15, 2013

Chen, Duan, Kang, Yang, Jiang, Zhang, Li, Yin, Hu, Dong, Li, Hajjar, Han: "Activation of liver X receptor induces macrophage interleukin-5 expression." in: The Journal of biological chemistry, Vol. 287, Issue 52, pp. 43340-50, 2012

Tong, Bai, Liu, Wang, Zhang, Xiao, Xu, Liu, Yang, Wu: "Expression, purification and monoclonal antibodies preparation of recombinant equine mature interleukin-18." in: Veterinary immunology and immunopathology, Vol. 136, Issue 3-4, pp. 194-200, 2010

Background publications

Le Beau, Lemons, Espinosa, Larson, Arai, Rowley: "Interleukin-4 and interleukin-5 map to human chromosome 5 in a region encoding growth factors and receptors and are deleted in myeloid leukemias with a del(5q)." in: Blood, Vol. 73, Issue 3, pp. 647-50, 1989

Images

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Supplier Images
ELISA image for IL5 ELISA Kit (Interleukin 5) (ABIN921063) Human IL-5 PicoKine ELISA Kit standard curve