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Mouse (Murine) IL5 ELISA Kit

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Antigen
Interleukin 5 (IL5) ELISA Kits
  • EDF
  • IL-5
  • Il-5
  • IL5
  • Pro-TRH
  • TRF
Epitope
AA 21-133
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
63
38
12
9
7
7
6
6
4
4
4
3
2
2
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN411302
$ 320.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN415513 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see 1
0.32693866 ABIN924872 Sandwich ELISA Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
0.32693866 ABIN1115669 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32693866 ABIN2708557 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.32693866 ABIN2708558 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.32693866 ABIN2064549 Cell Culture Supernatant, Serum, Plasma Log in to see
0.32693866 ABIN2762290 Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate), Plasma (heparin) 0.02-1.0 ng/mL 0.02-1.0 ng/mL Log in to see
0.32693866 ABIN2114330 Cell Culture Supernatant, Serum Log in to see
0.0012117105 ABIN365178 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012117105 ABIN3032818 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 2.1-500 pg/mL 2.1 pg/mL Log in to see
0.0012117105 ABIN3198461 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012117105 ABIN628000 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012117105 ABIN1029019 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012117105 ABIN1112652 Sandwich ELISA Serum, Body Fluids, Tissue Lysate, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN455597 Cell Culture Supernatant, Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN2703242 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 1-500 pg/mL 1 pg/mL Log in to see
0.0012117105 ABIN2114328 Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
0.0012117105 ABIN2748872 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN2792511 Cell Culture Supernatant, Serum, Plasma Log in to see
0.0012117105 ABIN2042556 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see

Top referenced IL5 ELISA Kit for ELISA

Similar IL5 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 36 ELISA Kits

General

Antigen Interleukin 5 (IL5) ELISA Kits
Epitope AA 21-133
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(63), (38), (12), (9), (7), (7), (6), (6), (4), (4), (4), (3), (2), (2)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 4 references available
Supplier Log in to see

Product Details IL5 ELISA Kit

Target details Application Details Handling References for IL5 Kit (ABIN411302) Images
Purpose For quantitative detection of mouse IL-5 in cell culture supernates and serum.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Detection Method Colorimetric
Specificity Natural and recombinant mouse IL-5
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <2pg/mL
Components
  • 96-well plate precoated with anti- mouse IL-5 antibod - 1
  • Lyophilized recombinant mouse IL-5 standar - 10ng/tubex2
  • Biotinylated anti- mouse IL-5 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: M21-G133

Target details

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Antigen
Alternative Name IL5 (IL5 ELISA Kit Abstract)
Background Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70 % amino acid sequence homology.
Gene ID 16191
UniProt P04401
Research Area Cancer, Cytokines, Innate Immunity, Adaptive Immunity
Pathways JAK-STAT Signaling

Application Details

Product Details IL5 ELISA Kit Target details Handling References for IL5 Kit (ABIN411302) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse IL-5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-5 has been precoated onto 96-well plates. Standards(sf21, M21-G133) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-5 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-5 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 96.3, Standard deviation: 5.68, CV(%): 5.9
  • Sample 2: n=16, Mean(pg/ml): 369, Standard deviation: 15.5, CV(%): 4.2
  • Sample 3: n=16, Mean(pg/ml): 721, Standard deviation: 24.51, CV(%): 3.4,
  • Sample 1: n=24, Mean(pg/ml): 110, Standard deviation: 7.26, CV(%): 6.6
  • Sample 2: n=24, Mean(pg/ml): 387, Standard deviation: 23.61, CV(%): 6.1
  • Sample 3: n=24, Mean(pg/ml): 675, Standard deviation: 37.8, CV(%): 5.6
Restrictions For Research Use only

Handling

Product Details IL5 ELISA Kit Target details Application Details References for IL5 Kit (ABIN411302) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL5 Kit (ABIN411302)

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Product cited in:

Li, Li, Zuo, Ke, Yan, Wen, Zhang, Yang: "T-helper type-2 contact hypersensitivity of Balb/c mice aggravated by dibutyl phthalate via long-term dermal exposure." in: PLoS ONE, Vol. 9, Issue 2, pp. e87887, 2014

Wang, Deng, Ren, Xiao, Chen, Tao: "Lactoferrin administration into the nostril alleviates murine allergic rhinitis and its mechanisms." in: Scandinavian journal of immunology, Vol. 78, Issue 6, pp. 507-15, 2013

Chen, Duan, Kang, Yang, Jiang, Zhang, Li, Yin, Hu, Dong, Li, Hajjar, Han: "Activation of liver X receptor induces macrophage interleukin-5 expression." in: The Journal of biological chemistry, Vol. 287, Issue 52, pp. 43340-50, 2012

Background publications

Le Beau, Lemons, Espinosa, Larson, Arai, Rowley: "Interleukin-4 and interleukin-5 map to human chromosome 5 in a region encoding growth factors and receptors and are deleted in myeloid leukemias with a del(5q)." in: Blood, Vol. 73, Issue 3, pp. 647-50, 1989

Images

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Supplier Images
ELISA image for IL5 ELISA Kit (Interleukin 5) (ABIN411302) Mouse IL-5 PicoKine ELISA Kit standard curve