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|Application / Reactivity||Mouse (Murine)|
|ELISA||36 ELISA Kits|
|Antigen||Interleukin 5 (IL5) ELISA Kits|
|Reactivity||Mouse (Murine) Alternatives|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||15.6-1000 pg/mL|
|Minimum Detection Limit||15.6 pg/mL|
|4 references available|
|Supplier||Log in to see|
Product Details IL5 ELISA KitTarget details Application Details Handling References for IL5 Kit (ABIN411302) Images
|Purpose||For quantitative detection of mouse IL-5 in cell culture supernates and serum.|
|Sample Type||Cell Culture Supernatant, Serum|
|Specificity||Natural and recombinant mouse IL-5|
|Cross-Reactivity (Details)||There is no detectable cross-reactivity with other relevant proteins.|
|Material not included||Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl|
Expression system for standard: sf21
Immunogen sequence: M21-G133
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|Background||Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70 % amino acid sequence homology.|
|Research Area||Cancer, Cytokines, Innate Immunity, Adaptive Immunity|
Application DetailsProduct Details IL5 ELISA Kit Target details Handling References for IL5 Kit (ABIN411302) Images back to top
|Application Notes||Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.|
|Plate||Pre-coated,Strips (12 x 8)|
|Protocol||mouse IL-5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-5 has been precoated onto 96-well plates. Standards(sf21, M21-G133) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-5 amount of sample captured in plate.|
|Assay Procedure||Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-5 standard solution and each sample be measured in duplicate.|
|Restrictions||For Research Use only|
HandlingProduct Details IL5 ELISA Kit Target details Application Details References for IL5 Kit (ABIN411302) Images back to top
|Storage||-20 °C,4 °C|
|Storage Comment||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles|
|Expiry Date||12 months|
References for IL5 Kit (ABIN411302)Product Details IL5 ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Li, Li, Zuo et al.: "T-helper type-2 contact hypersensitivity of Balb/c mice aggravated by dibutyl phthalate via long-term dermal exposure." in: PLoS ONE, Vol. 9, Issue 2, pp. e87887, 2014 (PubMed).
Wang, Deng, Ren et al.: "Lactoferrin administration into the nostril alleviates murine allergic rhinitis and its mechanisms." in: Scandinavian journal of immunology, Vol. 78, Issue 6, pp. 507-15, 2013 (PubMed).
Chen, Duan, Kang et al.: "Activation of liver X receptor induces macrophage interleukin-5 expression." in: The Journal of biological chemistry, Vol. 287, Issue 52, pp. 43340-50, 2012 (PubMed).
Le Beau, Lemons, Espinosa et al.: "Interleukin-4 and interleukin-5 map to human chromosome 5 in a region encoding growth factors and receptors and are deleted in myeloid leukemias with a del(5q)." in: Blood, Vol. 73, Issue 3, pp. 647-50, 1989 (PubMed).
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