anti-Human IL5RA antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended IL5RA Antibody (supplied by: Log in to see )

Antigen
Interleukin 5 Receptor, alpha (IL5RA) Antibodies
  • MGC86517
  • IL5RA
  • il5r
  • cdw125
  • hsil5r3
  • gpIL-5ra
  • CD125
  • CDw125
  • HSIL5R3
  • IL5R
  • Il5r
  • interleukin 5 receptor, alpha
  • il5ra
  • IL5RA
  • Il5ra
Reactivity
Human, Mouse (Murine)
103
37
25
2
2
2
2
2
2
1
Host
Rabbit
84
22
1
Clonality
Polyclonal
Conjugate
This IL5RA antibody is un-conjugated
8
6
6
6
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
83
44
30
23
13
6
2
2
2
1
1
Supplier
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Catalog No. ABIN498570
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN747833 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN651428 FACS IHC (p) WB Rabbit Ig AA 49-76, N-Term Log in to see Polyclonal
1 ABIN747842 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN747835 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN5536642 FACS IHC (p) WB Rabbit Ig Fraction AA 49-76, N-Term Log in to see Polyclonal
1 ABIN1812126 FACS IHC (p) WB Rabbit AA 49-76 Log in to see Polyclonal

General

Antigen Interleukin 5 Receptor, alpha (IL5RA) Antibodies
Reactivity Human, Mouse (Murine)
(103), (37), (25), (2), (2), (2), (2), (2), (2), (1)
Host Rabbit
(84), (22), (1)
Clonality
Polyclonal
Conjugate This IL5RA antibody is un-conjugated
(8), (6), (6), (6), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(83), (44), (30), (23), (13), (6), (2), (2), (2), (1), (1)
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Product Details anti-IL5RA Antibody

Target Details IL5RA Application Details Handling Images
Specificity This antibody detects endogenous levels of IL-5Ralpha protein. (region surrounding Lys68)
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse.
Purification Affinity Chromatography using epitope-specific immunogen

Target Details IL5RA

Product Details anti-IL5RA Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD125 / IL5RA (IL5RA Antibody Abstract)
Background Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cellreplacing factor (TRF), that induces B cell and eosinophil growth and differentiation. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of two chains: an alpha chain that binds IL-5 with low affinity and a beta chain that does not bind IL-5, but together with the IL-5 alpha chain, constitutes the high affinity IL-5 receptor. The cytoplasmic domain of both the alpha and beta chains is essential for signal transduction. Specifically, the membraneproximal proline-rich sequence of the cytoplasmic domain of the IL-5R receptor alpha subunit, IL-5Ralpha, is critical for the IL-5 induced proliferative response, expression of nuclear protooncogenes and tyrosine phosphorylation of cellular proteins, such as JAK1 and JAK2. Alternative splicing of the IL-5Ralpha gene produces several isoforms, including a membraneanchored isoform and a soluble isoform. The soluble isoform competes with IL-5 for binding to IL-5R and inhibits IL-5-mediated receptor activation and inflammatory mediator production, and, therefore, may be useful in treating asthma.Synonyms: IL-5R-alpha, IL5 Receptor alpha, Interleukin-5 receptor subunit alpha
Gene ID 3568
NCBI Accession NP_000555
UniProt Q01344
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL5RA Antibody Target Details IL5RA Handling Images back to top
Application Notes Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL5RA Antibody Target Details IL5RA Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-IL5RA Antibody Target Details IL5RA Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Interleukin 5 Receptor, alpha (IL5RA) antibody (ABIN498570) Immunohistochemistry analysis of CD125 / IL5RA Antibody in Paraffin-Embedded H...