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anti-Human IL7 antibody for Radioimmunoassay

Recommended IL7 Antibody (supplied by: Log in to see )

Antigen
Interleukin 7 (IL7) Antibodies
  • A630026I06Rik
  • hlb368
  • Il-7
  • IL-7
  • IL7
Reactivity
Human
159
60
31
1
Host
Rabbit
135
42
37
7
1
Clonality
Polyclonal
Conjugate
This IL7 antibody is un-conjugated
44
8
6
4
4
4
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Neutralization (Neut), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
146
137
43
29
15
14
12
11
9
6
5
4
4
3
2
2
2
2
2
1
1
Supplier
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Catalog No. ABIN964757
$ 369.00
Plus shipping costs $45.00

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General

Antigen Interleukin 7 (IL7) Antibodies
Reactivity Human
(159), (60), (31), (1)
Host Rabbit
(135), (42), (37), (7), (1)
Clonality
Polyclonal
Conjugate This IL7 antibody is un-conjugated
(44), (8), (6), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Neutralization (Neut), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
(146), (137), (43), (29), (15), (14), (12), (11), (9), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1)
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Product Details anti-IL7 Antibody

Target Details IL7 Application Details Handling Images
Specificity This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-7 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Characteristics Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues. Anti-IL-7 antibody is ideal for investigators involved in Immunology research.
Purification purified
Immunogen This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein.
Immunogen Type: RecombinantProtein
Isotype IgG

Target Details IL7

Product Details anti-IL7 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-7 (IL7 Antibody Abstract)
Background Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues. Anti-IL-7 antibody is ideal for investigators involved in Immunology research.
Synonyms: IL7, IL-7 Interleukin 7, Interleukin-7
Gene ID 3574
NCBI Accession NP_000871
UniProt P13232
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL7 Antibody Target Details IL7 Handling Images back to top
Application Notes This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 17.4 kDa in size corresponding to native human IL-7 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Comment

Gene Name: IL7

Restrictions For Research Use only

Handling

Product Details anti-IL7 Antibody Target Details IL7 Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

Images

Product Details anti-IL7 Antibody Target Details IL7 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-IL7 antibody (Interleukin 7) (ABIN964757) anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to r...