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anti-Human IL7 antibody for Western Blotting

Recommended IL7 Antibody (supplied by: Log in to see )

Antigen
Interleukin 7 (IL7) Antibodies
  • A630026I06Rik
  • hlb368
  • Il-7
  • IL-7
  • IL7
Reactivity
Human
182
61
31
1
Host
Rabbit
138
57
43
7
1
Clonality
Polyclonal
Conjugate
This IL7 antibody is conjugated to HRP
46
9
7
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
169
158
46
34
15
14
13
12
11
6
5
4
4
3
2
2
2
2
2
2
1
1
1
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Catalog No. ABIN964770
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN964779 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN3043249 ELISA WB Rabbit IgG AA 26-177 Log in to see Polyclonal
0.13341247 ABIN964757 IHC Neut RIA ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2706394 WB Rabbit Center Log in to see Polyclonal
0.13341247 ABIN3042805 ELISA WB Rabbit IgG AA 26-177 Log in to see Polyclonal
0.13341247 ABIN1873218 IHC WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN3022323 IHC WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN517057 WB Mouse AA 1-177, full length Log in to see Polyclonal
0.13341247 ABIN1078236 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 26-177 Log in to see Polyclonal
0.13341247 ABIN517059 WB Rabbit AA 1-177, full length Log in to see Polyclonal
0.13341247 ABIN517058 WB Mouse AA 1-177, full length Log in to see Polyclonal
0.13341247 ABIN2404464 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1102507 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN115765 EIA Func WB Mouse IgG1 Log in to see
0.0011728023 ABIN116086 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0011728023 ABIN1686094 Neut ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN2689783 ELISA WB Biotin Rat IgG2a Log in to see BVD10 2
0.0011728023 ABIN116088 EIA Func WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN116087 EIA Func WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN1102509 ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-IL7 antibody for Western Blotting

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General

Antigen Interleukin 7 (IL7) Antibodies
Reactivity Human
(182), (61), (31), (1)
Host Rabbit
(138), (57), (43), (7), (1)
Clonality
Polyclonal
Conjugate This IL7 antibody is conjugated to HRP
(46), (9), (7), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(169), (158), (46), (34), (15), (14), (13), (12), (11), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1)
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Product Details anti-IL7 Antibody

Target Details IL7 Application Details Handling Images
Specificity This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-7 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Characteristics Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.
Purification purified
Immunogen This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein.
Immunogen Type: RecombinantProtein
Isotype IgG

Target Details IL7

Product Details anti-IL7 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-7 (IL7 Antibody Abstract)
Background Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.
Synonyms: IL7, IL-7 Interleukin 7, Interleukin-7
Gene ID 3574
NCBI Accession NP_000871
UniProt P13232
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL7 Antibody Target Details IL7 Handling Images back to top
Application Notes This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 17.4 kDa in size corresponding to human IL-7 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Comment

Gene Name: IL7

Restrictions For Research Use only

Handling

Product Details anti-IL7 Antibody Target Details IL7 Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Gentamicin sulfate
Handling Advice Do NOT add Sodium Azide!
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

Images

Product Details anti-IL7 Antibody Target Details IL7 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-IL7 antibody (Interleukin 7)  (HRP) (ABIN964770) anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to r...