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anti-Mouse (Murine) IL7 antibody for Functional Studies

Recommended IL7 Antibody (supplied by: Log in to see )

Antigen
Interleukin 7 (IL7) Antibodies
  • A630026I06Rik
  • hlb368
  • Il-7
  • IL-7
  • IL7
Reactivity
Mouse (Murine)
182
60
31
1
Host
Rabbit
138
56
43
7
1
Clonality
Polyclonal
Conjugate
This IL7 antibody is un-conjugated
46
10
7
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
168
158
45
34
15
13
13
11
11
6
5
4
4
3
2
2
2
2
2
1
1
1
1
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Catalog No. ABIN115889
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087626296 ABIN115890 EIA Func WB Rabbit Log in to see Polyclonal
0.00087626296 ABIN208789 Func Neut ELISA WB Rabbit IgG Log in to see Polyclonal
0.00087626296 ABIN641034 Func ELISA WB Rabbit IgG Log in to see
0.00087626296 ABIN2475049 Func ELISA WB Rabbit IgG Log in to see Polyclonal

Similar anti-IL7 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 2 Antibodies
ELISA 27 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Functional Studies (Func) 5 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Neutralization (Neut) 10 Antibodies
Western Blotting (WB) 43 Antibodies

General

Antigen Interleukin 7 (IL7) Antibodies
Reactivity Mouse (Murine)
(182), (60), (31), (1)
Host Rabbit
(138), (56), (43), (7), (1)
Clonality
Polyclonal
Conjugate This IL7 antibody is un-conjugated
(46), (10), (7), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
(168), (158), (45), (34), (15), (13), (13), (11), (11), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-IL7 Antibody

Target Details IL7 Application Details Handling Images
Specificity This antibody recognizes Mouse Interleukin-7 (IL-7). Other species not tested.
Purification Affinity Chromatography immobilized Murine IL-7 matrix
Immunogen Highly pure (>98%) E.coli derived recombinant Mouse IL-7 (Cat.-No PA014)

Target Details IL7

Product Details anti-IL7 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin-7 / IL7 (IL7 Antibody Abstract)
Background Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.Synonyms: IL-7
Gene ID 16196
NCBI Accession NP_032397
UniProt P10168
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL7 Antibody Target Details IL7 Handling Images back to top
Application Notes Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-7 (0.60 ng/mL),a concentration of 0.012 - 0.02 μg/mL of this antibody is required. ELISA: To detect mIL-7 by direct ELISA (using 100 l/well antibody solution) a concentration of atleast 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant mIL-7. Western Blot: To detect mIL-7 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-IL7 Antibody Target Details IL7 Application Details Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.