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|Antigen||Interleukin 9 (IL9) Antibodies|
|Conjugate||This IL9 antibody is conjugated to Biotin Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-IL9 AntibodyTarget Details IL9 Application Details Handling Images
|Immunogen||Highly pure (>98%) recombinant hIL-9 (human IL-9).|
Target Details IL9Product Details anti-IL9 Antibody Application Details Handling Images back to top
|Alternative Name||Interleukin-9 / IL9 (IL9 Antibody Abstract)|
|Background||Interleukin 9 (IL9) is a cytokine preferentially produced by T helper type 2 lymphocytes and active on various cell types such as T and B lymphocytes, mast cells and haemopoietic progenitors, but no effect of this cytokine has been reported so far on mononuclear phagocytes. IL9 stimulates cell proliferation and prevents apoptosis. The IL9 receptor (IL 9R) belongs to the haemopoietic receptor superfamily and its signal transduction involves mainly the (JAK/STAT) pathway. Some studies have implicated IL9 as an important cytokine in a number of Th2 cytokine mediated pathologies, in particular asthma.Synonyms: IL-9, P40 cytokine, T-cell growth factor P40|
Application DetailsProduct Details anti-IL9 Antibody Target Details IL9 Handling Images back to top
ELISA: To detect hIL-9 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-9. Western Blot: To detect hIL-9 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-9 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL9 Antibody Target Details IL9 Application Details Images back to top
|Reconstitution||Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing. Centrifuge vial prior to opening!|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody at 4-8 °C for one month or at -20 °C for longer. This product should be stored undiluted. This product is photosensitive and should be protected from the light.|