anti-Mouse (Murine) IL9 antibody for ELISA

Recommended IL9 Antibody (supplied by: Log in to see )

Antigen
Interleukin 9 (IL9) Antibodies
  • IL9
  • IL-9
  • Il-9
  • P40
  • HP40
  • interleukin 9
  • interleukin 9-like
  • IL9
  • il9
  • Il9
  • LOC100340820
Reactivity
Mouse (Murine)
108
85
27
1
1
1
1
Host
Armenian Hamster
105
42
39
5
3
1
Clonality
Monoclonal
Conjugate
This IL9 antibody is un-conjugated
21
9
7
7
6
6
5
5
5
5
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Neutralization (Neut), ELISA
100
65
65
24
20
13
8
6
6
4
4
4
4
2
2
2
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN1176989
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN465852 ELISA Neut WB Biotin Rabbit Log in to see Polyclonal
1 ABIN2689792 ELISA Biotin Armenian Hamster IgG2, kappa Log in to see D9302C12 2
1 ABIN465833 Neut ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2689790 ELISA Rat IgG2a Log in to see D8402E8 2
1 ABIN1002670 Inhibition ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2289990 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN289359 Inhibition ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2289997 Neut ELISA WB APC Rabbit IgG Log in to see Polyclonal
1 ABIN2289999 Neut ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN2289995 Neut ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal
1 ABIN2290001 Neut ELISA WB FITC Rabbit IgG Log in to see Polyclonal
1 ABIN2290005 Neut ELISA WB PE Rabbit IgG Log in to see Polyclonal
1 ABIN2290003 Neut ELISA WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN2289993 Neut ELISA WB Rabbit IgG Log in to see Polyclonal

General

Antigen Interleukin 9 (IL9) Antibodies
Reactivity Mouse (Murine)
(108), (85), (27), (1), (1), (1), (1)
Host Armenian Hamster
(105), (42), (39), (5), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL9 antibody is un-conjugated
(21), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Neutralization (Neut), ELISA
(100), (65), (65), (24), (20), (13), (8), (6), (6), (4), (4), (4), (4), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-IL9 Antibody

Target Details IL9 Application Details Handling ProductDetails: References for anti-IL9 antibody (ABIN1176989) Images
Brand BD Pharmingen™
Characteristics Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-9. Neutralization activity may be measured with a proliferation assay using 500 pg/mL recombinant mouse IL-9 to stimulate MC/9 cells at 5x10^4 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-9 for 60 minutes).
50% Neutralization (ND50) at 5 - 25 ng/mL
>95% Neutralization at 50 - 250 ng/mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Mouse IL-9 Recombinant Protein
Clone D9302C12
Isotype IgG2, kappa

Target Details IL9

Product Details anti-IL9 Antibody Application Details Handling ProductDetails: References for anti-IL9 antibody (ABIN1176989) Images back to top
Antigen
Alternative Name IL-9 (IL9 Antibody Abstract)
Background The D9302C12 monoclonal antibody specifically binds to the multifunctional mouse cytokine, Interleukin-9 (IL-9). IL-9 is a 126 amino acid-long glycoprotein that is produced by various subsets of activated CD4+ T cells. IL-9 acts on target cells by binding to and signaling through the heterodimeric IL-9 receptor (IL-9R) complex that is comprised of transmembrane IL-9 receptor alpha (IL-9Ralpha) and common gamma chain (gammac) subunits. IL-9 can promote the survival, growth, proliferation and/or differentiation of various cell types including thymocytes, T cells, B cells, mast cells, and hematopoietic progenitor cells. IL-9 can augment IL-4-induced IgE and IgG1 production from lipopolysaccharide-primed mouse B cells and induce granzyme and high-affinity IgE receptor gene expression by mouse T helper cell clones and mast cell lines. IL-9 plays an important role in vivo in helminth elimination. The D9302C12 antibody neutralizes mouse IL-9 bioactivity.
Synonyms: IL-9, Interleukin-9, MEA, P40, T-cell growth factor P40, TCGF III
Research Area Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL9 Antibody Target Details IL9 Handling ProductDetails: References for anti-IL9 antibody (ABIN1176989) Images back to top
Application Notes ELISA: Biotinylated D9302C12 antibody (Cat. No. 554473) is useful as a detection antibody in sandwich ELISA for measuring mouse IL-9 protein levels. Biotinylated D9302C12 antibody can be paired with the purified D8402E8 antibody (Cat. No. 551218) as the capture antibody, with recombinant mouse IL-9 as the standard.
Restrictions For Research Use only

Handling

Product Details anti-IL9 Antibody Target Details IL9 Application Details ProductDetails: References for anti-IL9 antibody (ABIN1176989) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

ProductDetails: References for anti-IL9 antibody (ABIN1176989)

Product Details anti-IL9 Antibody Target Details IL9 Application Details Handling Images back to top
Product cited in:

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, 2008

Renauld, Kermouni, Vink, Louahed, Van Snick: "Interleukin-9 and its receptor: involvement in mast cell differentiation and T cell oncogenesis." in: Journal of leukocyte biology, Vol. 57, Issue 3, pp. 353-60, 1995

Nabel, Galli, Dvorak, Dvorak, Cantor: "Inducer T lymphocytes synthesize a factor that stimulates proliferation of cloned mast cells." in: Nature, Vol. 291, Issue 5813, pp. 332-4, 1981