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anti-Mouse (Murine) IL9 antibody for Neutralization

Recommended IL9 Antibody (supplied by: Log in to see )

Antigen
Interleukin 9 (IL9) Antibodies
  • HP40
  • Il-9
  • IL-9
  • IL9
  • P40
Reactivity
Mouse (Murine)
100
60
19
1
Host
Armenian Hamster
100
32
22
4
3
2
Clonality
Monoclonal
Conjugate
This IL9 antibody is un-conjugated
20
7
6
6
6
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Neutralization (Neut), ELISA
80
64
51
21
19
11
8
5
5
5
4
4
3
3
2
2
2
2
1
Supplier
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Catalog No. ABIN1176989
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008737065 ABIN465833 Neut ELISA WB Rabbit Log in to see Polyclonal
0.0008737065 ABIN2289997 Neut ELISA WB APC Rabbit IgG Log in to see Polyclonal
0.0008737065 ABIN2289999 Neut ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.0008737065 ABIN2290003 Neut ELISA WB HRP Rabbit IgG Log in to see Polyclonal
0.0008737065 ABIN2290005 Neut ELISA WB PE Rabbit IgG Log in to see Polyclonal
0.0008737065 ABIN2290001 Neut ELISA WB FITC Rabbit IgG Log in to see Polyclonal
0.0008737065 ABIN2289995 Neut ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal
0.0008737065 ABIN2289993 Neut ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-IL9 antibody for Neutralization

Similar anti-IL9 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 2 Antibodies
ELISA 15 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 23 Antibodies
Functional Studies (Func) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies
Neutralization (Neut) 9 Antibodies
Western Blotting (WB) 21 Antibodies

General

Antigen Interleukin 9 (IL9) Antibodies
Reactivity Mouse (Murine)
(100), (60), (19), (1)
Host Armenian Hamster
(100), (32), (22), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL9 antibody is un-conjugated
(20), (7), (6), (6), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Neutralization (Neut), ELISA
(80), (64), (51), (21), (19), (11), (8), (5), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-IL9 Antibody

Target Details IL9 Application Details Handling References for anti-IL9 Antibody (ABIN1176989) Images
Brand BD Pharmingen™
Characteristics Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-9. Neutralization activity may be measured with a proliferation assay using 500 pg/mL recombinant mouse IL-9 to stimulate MC/9 cells at 5x10^4 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-9 for 60 minutes).
50% Neutralization (ND50) at 5 - 25 ng/mL
>95% Neutralization at 50 - 250 ng/mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Mouse IL-9 Recombinant Protein
Clone D9302C12
Isotype IgG2, kappa

Target Details IL9

Product Details anti-IL9 Antibody Application Details Handling References for anti-IL9 Antibody (ABIN1176989) Images back to top
Antigen
Alternative Name IL-9 (IL9 Antibody Abstract)
Background The D9302C12 monoclonal antibody specifically binds to the multifunctional mouse cytokine, Interleukin-9 (IL-9). IL-9 is a 126 amino acid-long glycoprotein that is produced by various subsets of activated CD4+ T cells. IL-9 acts on target cells by binding to and signaling through the heterodimeric IL-9 receptor (IL-9R) complex that is comprised of transmembrane IL-9 receptor alpha (IL-9Ralpha) and common gamma chain (gammac) subunits. IL-9 can promote the survival, growth, proliferation and/or differentiation of various cell types including thymocytes, T cells, B cells, mast cells, and hematopoietic progenitor cells. IL-9 can augment IL-4-induced IgE and IgG1 production from lipopolysaccharide-primed mouse B cells and induce granzyme and high-affinity IgE receptor gene expression by mouse T helper cell clones and mast cell lines. IL-9 plays an important role in vivo in helminth elimination. The D9302C12 antibody neutralizes mouse IL-9 bioactivity.
Synonyms: IL-9, Interleukin-9, MEA, P40, T-cell growth factor P40, TCGF III
Research Area Cancer
Pathways JAK-STAT Signaling, TCR Signaling

Application Details

Product Details anti-IL9 Antibody Target Details IL9 Handling References for anti-IL9 Antibody (ABIN1176989) Images back to top
Application Notes ELISA: Biotinylated D9302C12 antibody (Cat. No. 554473) is useful as a detection antibody in sandwich ELISA for measuring mouse IL-9 protein levels. Biotinylated D9302C12 antibody can be paired with the purified D8402E8 antibody (Cat. No. 551218) as the capture antibody, with recombinant mouse IL-9 as the standard.
Restrictions For Research Use only

Handling

Product Details anti-IL9 Antibody Target Details IL9 Application Details References for anti-IL9 Antibody (ABIN1176989) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL9 Antibody (ABIN1176989)

Product Details anti-IL9 Antibody Target Details IL9 Application Details Handling Images back to top
Product cited in:

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, 2008

Renauld, Kermouni, Vink, Louahed, Van Snick: "Interleukin-9 and its receptor: involvement in mast cell differentiation and T cell oncogenesis." in: Journal of leukocyte biology, Vol. 57, Issue 3, pp. 353-60, 1995

Nabel, Galli, Dvorak, Dvorak, Cantor: "Inducer T lymphocytes synthesize a factor that stimulates proliferation of cloned mast cells." in: Nature, Vol. 291, Issue 5813, pp. 332-4, 1981