anti-Human IL9 Receptor antibody for Functional Studies

Recommended IL9 Receptor Antibody (supplied by: Log in to see )

Antigen
Interleukin 9 Receptor (IL9R) Antibodies
  • CD129
  • IL-9R
  • interleukin 9 receptor
  • IL9R
  • Il9r
Epitope
Extracellular Domain
17
10
9
8
8
5
3
3
3
2
1
1
1
1
1
1
1
1
Reactivity
Human
117
29
4
Host
Mouse
77
45
16
Clonality
Monoclonal
Conjugate
This IL9 Receptor antibody is un-conjugated
10
6
6
6
6
5
5
4
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Neutralization (Neut), ELISA
65
59
58
30
10
6
5
5
2
2
1
1
1
1
Supplier
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Catalog No. ABIN327384
$ 1,079.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
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General

Antigen Interleukin 9 Receptor (IL9R) Antibodies
Epitope Extracellular Domain
(17), (10), (9), (8), (8), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(117), (29), (4)
Host Mouse
(77), (45), (16)
Clonality
Monoclonal
Conjugate This IL9 Receptor antibody is un-conjugated
(10), (6), (6), (6), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Neutralization (Neut), ELISA
(65), (59), (58), (30), (10), (6), (5), (5), (2), (2), (1), (1), (1), (1)
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Product Details anti-IL9 Receptor Antibody

Target Details IL9 Receptor Application Details Handling Images
Specificity Recognizes human IL-9R. In direct ELISAs, this antibody does not cross-react with rhIL-4 R, rhIL-5 R, rhIL-5 R, rhIL-13 R 1, rhIL-13 R 2, or rmIL-9 R.
Purification Protein G purified
Immunogen Sf21-derived rhIL-9 R extracellular domain.

Type of Immunogen: Recombinant protein
Isotype IgG1

Target Details IL9 Receptor

Product Details anti-IL9 Receptor Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL9R / CD129 (IL9R Antibody Abstract)
Background Name/Gene ID: IL9R
Family: Interleukin

Synonyms: IL9R, IL-9 receptor, IL-9R, Interleukin 9 receptor, Interleukin-9 receptor, CD129, CD129 antigen
Gene ID 3581
UniProt Q01113
Pathways JAK-STAT Signaling, Growth Factor Binding

Application Details

Product Details anti-IL9 Receptor Antibody Target Details IL9 Receptor Handling Images back to top
Application Notes Approved: ELISA, Flo, Func, Neut

Usage: Neutralization of bioactivity, Flow Cytometry, Direct ELISA. Neutralization of Human IL-9 Receptor-mediated bioactivity: The exact concentration of antibody required to neutralize the human cell surface IL-9 R mediated bioactivity is dependent on the IL-2 concentration as well as on the number and types of IL-2 receptors present on the cell surface (a function of cell type and culture conditions). The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-9 R mediated IL-9 response on a responsive cell line, at a specific IL-9 concentration. The ND50 for this lot of anti-human IL-9 R antibody was determined to be approximately 2-4 μg/mL for inhibiting the IL-9-dependent proliferation of MO7e cells (1 x 10^5 cells/mL) in the presence of 2 ng/mL of rhIL-9. Flow Cytometry: This antibody was validated for flow cytometry using blood-derived monocytes. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human IL-9 R. The detection limit for rhIL-9 R is approximately 6 ng/well.
Restrictions For Research Use only

Handling

Product Details anti-IL9 Receptor Antibody Target Details IL9 Receptor Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.
Concentration Lot specific
Buffer Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.