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anti-Mouse (Murine) JAK2 antibody for Immunohistochemistry (Frozen Sections)

Recommended JAK2 Antibody (supplied by: Log in to see )

Antigen
Janus Kinase 2 (JAK2) Antibodies
  • AI504024
  • C81284
  • Fd17
  • JAK-2
  • jak2
  • JAK2bCA
  • JTK10
  • THCYT3
Epitope
Tyr1007, Tyr1008
46
27
18
17
13
12
11
11
9
8
7
7
5
4
4
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
240
158
147
8
7
6
6
2
1
1
1
Host
Rabbit
236
14
5
Clonality
Monoclonal
Conjugate
This JAK2 antibody is un-conjugated
9
6
6
2
2
2
2
2
2
2
2
2
2
2
2
Application
Dot Blot (DB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
168
110
96
30
25
23
17
9
5
4
3
3
Supplier
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Available images

Catalog No. ABIN189473
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088254584 ABIN1859523 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 564-809 Log in to see Polyclonal
0.00088254584 ABIN1176454 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 120-367 Log in to see Polyclonal

Similar anti-JAK2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 43 Antibodies 47 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 13 Antibodies 15 Antibodies
Immunofluorescence (IF) 14 Antibodies 20 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 77 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 17 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 87 Antibodies
Flow Cytometry (FACS) 3 Antibodies

General

Antigen Janus Kinase 2 (JAK2) Antibodies
Epitope Tyr1007, Tyr1008
(46), (27), (18), (17), (13), (12), (11), (11), (9), (8), (7), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(240), (158), (147), (8), (7), (6), (6), (2), (1), (1), (1)
Host Rabbit
(236), (14), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This JAK2 antibody is un-conjugated
(9), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Dot Blot (DB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(168), (110), (96), (30), (25), (23), (17), (9), (5), (4), (3), (3)
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Product Details anti-JAK2 Antibody

Target Details JAK2 Application Details Handling Images
Specificity The antibody only detects JAK2 phosphorylated on Tyrosine 1007 and 1008.
Purification IgG purified
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr1007 and Tyr1008 of human JAK2 was used as immunogen.
Clone E132
Isotype IgG

Target Details JAK2

Product Details anti-JAK2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Tyrosine-protein kinase JAK2 (JAK2 Antibody Abstract)
Background Jak2 is a member of the Janus family tyrosine kinases (Jaks), whose function is totransduce extracellular signals from cytokines and interferons. Jaks associate withcytokine receptors, upon ligand binding, Jaks phosphorylate the receptor. Thephosphorylated receptors are bound by SH2-containing proteins, one class of which isthe signal transducers and activators of transcription (STATs). Activated STATs, then,translocate to the nucleus to mediate gene transcription (1,2). Tyr1007/1008 of Jak2 arethe homologous Tyr1054/1055 in Tyk2, and phosphorylation at these residues isneeded for Tyk2 activation (3). A chromosomal aberration involving Jak2 is found in aform of pre-B acute myeloid leukemia (4). Alternate Names: anti-JAK 2 antibody, anti-Janus Activating Kinase 2 antibody, anti-Janus Kinase 2antibody, anti-OTTHUMP00000043260 antibody, anti-Tyrosine protein kinase JAK2antibody.
Gene Symbol: JAK2
Gene ID 3717, 16452, 24514
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-JAK2 Antibody Target Details JAK2 Handling Images back to top
Application Notes Use in IHC-F was reported in the scientific literature (PMID: 21420414)
Recommended dilutions: Dot Blot 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:50-100, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Frozen 1:10-1:2000, Immunohistochemistry-Paraffin 1:50-100, Immunoprecipitation 1:50, Western Blot 1:1000-10000
Protocol IHC Protocol specific for JAK2 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-JAK2 Antibody Target Details JAK2 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-JAK2 Antibody Target Details JAK2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-JAK2 antibody (Janus Kinase 2) (Tyr1007) (ABIN189473) Western blot analysis of immunoprecipitation pellets from Jurkat cell lysate +GM-CSF ...
Immunohistochemistry (IHC) image for anti-JAK2 antibody (Janus Kinase 2) (Tyr1007) (ABIN189473) Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using ant...
Western Blotting (WB) image for anti-JAK2 antibody (Janus Kinase 2) (Tyr1007) (ABIN189473) Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using ...