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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Dot Blot (DB)||1 Antibodies||1 Antibodies|
|ELISA||41 Antibodies||45 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||2 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||11 Antibodies||12 Antibodies|
|Immunofluorescence (IF)||12 Antibodies||17 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||24 Antibodies||24 Antibodies|
|Immunohistochemistry (IHC)||72 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||13 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Western Blotting (WB)||82 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies|
|Antigen||Janus Kinase 2 (JAK2) Antibodies|
|Epitope||Tyr1007, Tyr1008 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This JAK2 antibody is un-conjugated Alternatives|
Dot Blot (DB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-JAK2 AntibodyTarget Details JAK2 Application Details Handling Images
|Specificity||The antibody only detects JAK2 phosphorylated on Tyrosine 1007 and 1008.|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Tyr1007 and Tyr1008 of human JAK2 was used as immunogen.|
Target Details JAK2Product Details anti-JAK2 Antibody Application Details Handling Images back to top
|Alternative Name||Tyrosine-protein kinase JAK2 (JAK2 Antibody Abstract)|
Jak2 is a member of the Janus family tyrosine kinases (Jaks), whose function is totransduce extracellular signals from cytokines and interferons. Jaks associate withcytokine receptors, upon ligand binding, Jaks phosphorylate the receptor. Thephosphorylated receptors are bound by SH2-containing proteins, one class of which isthe signal transducers and activators of transcription (STATs). Activated STATs, then,translocate to the nucleus to mediate gene transcription (1,2). Tyr1007/1008 of Jak2 arethe homologous Tyr1054/1055 in Tyk2, and phosphorylation at these residues isneeded for Tyk2 activation (3). A chromosomal aberration involving Jak2 is found in aform of pre-B acute myeloid leukemia (4). Alternate Names: anti-JAK 2 antibody, anti-Janus Activating Kinase 2 antibody, anti-Janus Kinase 2antibody, anti-OTTHUMP00000043260 antibody, anti-Tyrosine protein kinase JAK2antibody.
Gene Symbol: JAK2
|Gene ID||3717, 16452, 24514|
|Pathways||JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway|
Application DetailsProduct Details anti-JAK2 Antibody Target Details JAK2 Handling Images back to top
Use in IHC-F was reported in the scientific literature (PMID: 21420414)
Recommended dilutions: Dot Blot 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:50-100, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Frozen 1:10-1:2000, Immunohistochemistry-Paraffin 1:50-100, Immunoprecipitation 1:50, Western Blot 1:1000-10000
IHC Protocol specific for JAK2 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-JAK2 Antibody Target Details JAK2 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-JAK2 Antibody Target Details JAK2 Application Details Handling back to top