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|Antigen||Tyrosine-Protein Kinase JAK3 (JAK3) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This JAK3 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-JAK3 AntibodyTarget Details JAK3 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of JAK3 protein.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % (by SDS-PAGE)|
Target Details JAK3Product Details anti-JAK3 Antibody Application Details Handling Images back to top
|Alternative Name||Tyrosine-Protein Kinase JAK3 (JAK3 Antibody Abstract)|
|Background||Jak3 (Janus Kinase 3) belongs to the family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK3. JAK3 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system, stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies.Synonyms: JAK-3, Janus kinase 3, L-JAK, Leukocyte janus kinase|
|Molecular Weight||approx. 125 kDa|
|Research Area||Signaling, Cytokines, Receptors, Kinases/Phosphatases|
|Pathways||JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded protein response|
Application DetailsProduct Details anti-JAK3 Antibody Target Details JAK3 Handling Images back to top
Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.|
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