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anti-Mouse (Murine) JAK3 antibody for Flow Cytometry

Recommended JAK3 Antibody (supplied by: Log in to see )

Antigen
Tyrosine-Protein Kinase JAK3 (JAK3) Antibodies
  • fae
  • JAK
  • JAK-3
  • JAK3_HUMAN
  • JAKL
  • L-JAK
  • LJAK
  • RATJAK3
  • wil
Reactivity
Human, Mouse (Murine)
83
57
41
2
2
2
2
1
1
1
Host
Mouse
64
22
1
Clonality
Monoclonal
Conjugate
This JAK3 antibody is un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
50
37
23
15
12
11
7
6
2
1
1
1
1
Supplier
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Catalog No. ABIN969224
$ 345.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN1844119 ICC FACS IF ELISA WB Mouse IgG1 Log in to see
0.0010482287 ABIN1583685 FACS IF WB Mouse IgG1 Log in to see 5H2
0.0010482287 ABIN4327888 ELISA FACS ICC IF WB Mouse IgG1 Log in to see 5H2
0.0010482287 ABIN1577384 FACS IF ELISA WB Mouse Log in to see

Top referenced anti-JAK3 antibody for Flow Cytometry

Similar anti-JAK3 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 16 Antibodies 37 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 5 Antibodies 8 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 7 Antibodies
Immunofluorescence (IF) 14 Antibodies 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 12 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 36 Antibodies
ELISA (Capture) 1 Antibodies

General

Antigen Tyrosine-Protein Kinase JAK3 (JAK3) Antibodies
Reactivity Human, Mouse (Murine)
(83), (57), (41), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(64), (22), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This JAK3 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(50), (37), (23), (15), (12), (11), (7), (6), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-JAK3 Antibody

Target Details JAK3 Application Details Handling References for anti-JAK3 Antibody (ABIN969224) Images
Purification Ascites
Immunogen Purified recombinant fragment of human JAK3 expressed in E. coli.
Clone 5H2
Isotype IgG1

Target Details JAK3

Product Details anti-JAK3 Antibody Application Details Handling References for anti-JAK3 Antibody (ABIN969224) Images back to top
Antigen
Alternative Name JAK3 (JAK3 Antibody Abstract)
Background JAK3, Janus kinase 3. It is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease).
Molecular Weight 125 kDa
Gene ID 3718
HGNC 3718
Research Area Signaling, Cytokines, Receptors, Kinases/Phosphatases
Pathways JAK-STAT Signaling, RTK Signaling

Application Details

Product Details anti-JAK3 Antibody Target Details JAK3 Handling References for anti-JAK3 Antibody (ABIN969224) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-JAK3 Antibody Target Details JAK3 Application Details References for anti-JAK3 Antibody (ABIN969224) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-JAK3 Antibody (ABIN969224)

Product Details anti-JAK3 Antibody Target Details JAK3 Application Details Handling Images back to top
Product cited in:

Wood, Goodman, Uckun: "Jak3 expression and genomic sequence in pediatric acute lymphoblastic leukemia." in: Leukemia & lymphoma, Vol. 43, Issue 12, pp. 2355-62, 2003

Background publications

Lai, Jin, Graham, Witthuhn, Ihle, Liu: "A kinase-deficient splice variant of the human JAK3 is expressed in hematopoietic and epithelial cancer cells." in: The Journal of biological chemistry, Vol. 270, Issue 42, pp. 25028-36, 1995

Kawamura, McVicar, Johnston, Blake, Chen, Lal, Lloyd, Kelvin, Staples, Ortaldo: "Molecular cloning of L-JAK, a Janus family protein-tyrosine kinase expressed in natural killer cells and activated leukocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 14, pp. 6374-8, 1994

Images

Product Details anti-JAK3 Antibody Target Details JAK3 Application Details Handling References for anti-JAK3 Antibody (ABIN969224) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-JAK3 antibody (Tyrosine-Protein Kinase JAK3) (ABIN969224) Figure 1: Flow cytometric analysis of Hela cells using JAK3 mouse mAb (blue) and nega...
Immunocytochemistry (ICC) image for anti-JAK3 antibody (Tyrosine-Protein Kinase JAK3) (ABIN969224) Figure 2: Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells...
Immunocytochemistry (ICC) image for anti-JAK3 antibody (Tyrosine-Protein Kinase JAK3) (ABIN969224) Figure 3: Confocal immunofluorescence analysis of 3T3-L1 cells using JAK3 mouse mAb (...
Western Blotting (WB) image for anti-JAK3 antibody (Tyrosine-Protein Kinase JAK3) (ABIN969224) Figure 4: Western blot analysis using JAK3 mouse mAb against Jurkat cell lysate (1) .