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anti-Cow (Bovine) Leptin antibody for Immunocytochemistry

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
AA 29-160
30
23
18
15
15
15
11
6
6
6
6
6
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine)
415
168
144
21
6
4
4
4
3
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
353
211
19
8
4
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
64
23
17
11
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
409
404
97
56
46
39
32
26
20
19
17
10
10
6
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
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Available images

Catalog No. ABIN1862847
$ 415.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3201292 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see

Similar anti-Leptin Antibodies

Application / Reactivity Cow (Bovine)
ELISA 3 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 10 Antibodies

General

Antigen Leptin (LEP) Antibodies
Epitope AA 29-160
(30), (23), (18), (15), (15), (15), (11), (6), (6), (6), (6), (6), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine)
(415), (168), (144), (21), (6), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(353), (211), (19), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(64), (23), (17), (11), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(409), (404), (97), (56), (46), (39), (32), (26), (20), (19), (17), (10), (10), (6), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Specificity The antibody is a rabbit polyclonal antibody raised against LEP. It has been selected for its ability to recognize LEP in immunohistochemical staining and western blotting.
Purification Antigen-specific affinity chromatography
Immunogen LEP (Asp29-Gln160)
Isotype IgG

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Background Alternative Names: OB, OBS, Obesity Homolog, Obesity Factor, Obese Protein
Research Area Hormones
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes
  • Western blotting: 0.5-2 μg/mL
  • Immunocytochemistry in formalin fixed cells: 5-20 μg/mL
  • Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL
  • Immunohistochemistry in paraffin section: 5-20 μg/mL
  • Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL
  • Optimal working dilutions must be determined by end user.
Comment

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Liquid
Concentration 200 μg/mL
Buffer 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Leptin antibody (LEP) (AA 29-160) (ABIN1862847) anti-Leptin (LEP) (AA 29-160) antibody