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anti-Horse (Equine) Leptin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • LEPD
  • OB
  • ob
  • obese
  • OBS
Epitope
AA 15-141
24
22
21
13
13
11
9
8
6
6
6
6
6
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Horse (Equine)
332
151
131
18
2
1
1
Host
Rabbit
348
134
20
8
4
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
60
19
13
7
7
5
5
3
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
336
321
69
39
38
33
26
25
17
16
16
12
10
7
5
3
3
3
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1859646
$ 426.80
Plus shipping costs $45.00

Similar anti-Leptin Antibodies

Application / Reactivity Horse (Equine)
ELISA 2 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Western Blotting (WB) 1 Antibodies

General

Antigen Leptin (LEP) Antibodies
Epitope AA 15-141
(24), (22), (21), (13), (13), (11), (9), (8), (6), (6), (6), (6), (6), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Horse (Equine)
(332), (151), (131), (18), (2), (1), (1)
Host Rabbit
(348), (134), (20), (8), (4)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(60), (19), (13), (7), (7), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(336), (321), (69), (39), (38), (33), (26), (25), (17), (16), (16), (12), (10), (7), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IRKVQD DTKTLIKTIV TRINDISHTQ SVSSKQRVTG LDFIPGLHPV LSLSKMDQTL AIYQQILTSL PSRNVIQISN DLENLRDLLH LLASSKSCPL PQARGLETLA SLGGVLEASL LLHRGGSPEQ A
Specificity It has been selected for its ability to recognize LEP in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen LEP (AA 15-141)
Isotype IgG

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Research Area Hormones
Pathways JAK-STAT Signaling, AMPK Signaling

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant LEP (Ile15~Ala141) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Leptin antibody (LEP) (AA 15-141) (ABIN1859646) anti-Leptin (LEP) (AA 15-141) antibody