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anti-Human Leptin antibody for Enzyme Immunoassay

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
C-Term, AA 138-167
31
23
18
15
15
15
11
6
6
6
6
6
6
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
410
167
146
24
8
6
5
4
4
4
3
3
2
2
1
1
1
1
1
1
Host
Rabbit
353
207
19
8
4
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
64
23
17
11
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
405
399
98
56
46
39
31
25
21
19
17
10
10
6
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN358628
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN953161 EIA FACS IF IHC (p) WB Rabbit Ig AA 7-37, N-Term Log in to see Polyclonal 2
1 ABIN318591 EIA Sheep IgG AA 57-70 Log in to see Polyclonal 1
1 ABIN114695 EIA WB Mouse IgG1 Log in to see LEP-02 6
1 ABIN115495 EIA IHC (fro) IP WB Rabbit Log in to see Polyclonal 5
1 ABIN116129 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN116130 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN116132 EIA WB Rabbit Log in to see Polyclonal
1 ABIN181148 EIA WB Mouse IgG1 Log in to see
1 ABIN116131 EIA WB Rabbit Log in to see Polyclonal
1 ABIN302917 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN613247 EIA RIA Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN613721 EIA RIA Rabbit IgG Log in to see Polyclonal

Top referenced anti-Leptin antibody for Enzyme Immunoassay

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Application / Reactivity Human
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Blocking Peptide (BP) 1 Antibodies
Cell Culture (CC) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 290 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 4 Antibodies
Enzyme Immunoassay (EIA) 13 Antibodies
Flow Cytometry (FACS) 19 Antibodies
Immunoassay (IA) 4 Antibodies
Immunochromatography (IC) 15 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 46 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 77 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies

General

Antigen Leptin (LEP) Antibodies
Epitope C-Term, AA 138-167
(31), (23), (18), (15), (15), (15), (11), (6), (6), (6), (6), (6), (6), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(410), (167), (146), (24), (8), (6), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(353), (207), (19), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(64), (23), (17), (11), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(405), (399), (98), (56), (46), (39), (31), (25), (21), (19), (17), (10), (10), (6), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling References for anti-Leptin Antibody (ABIN358628) Images
Specificity This antibody recognizes Human Leptin (LEP).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen KLH conjugated synthetic peptide between 138~167 amino acids from the C-terminal region of Human Leptin . Genename: LEP
Isotype Ig

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling References for anti-Leptin Antibody (ABIN358628) Images back to top
Antigen
Alternative Name Leptin (LEP Antibody Abstract)
Background Leptin (LEP) is an adipocyte-secreted protein that regulates body fat and endocrine functions. Leptin is similar to the mouse obesity protein encoded by the obese gene (ob). In mouse studies, mutations in this gene are linked to severe and morbid obesity.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor
Gene ID 3952, 9606
UniProt P41159
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling References for anti-Leptin Antibody (ABIN358628) Images back to top
Application Notes ELISA: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details References for anti-Leptin Antibody (ABIN358628) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-Leptin Antibody (ABIN358628)

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling Images back to top
Background publications

Tank, Jordan, Diedrich, Schroeder, Furlan, Sharma, Luft, Brabant: "Bound leptin and sympathetic outflow in nonobese men." in: The Journal of clinical endocrinology and metabolism, Vol. 88, Issue 10, pp. 4955-9, 2003

Kazumi, Kawaguchi, Hirano, Yoshino: "C-reactive protein in young, apparently healthy men: associations with serum leptin, QTc interval, and high-density lipoprotein-cholesterol." in: Metabolism: clinical and experimental, Vol. 52, Issue 9, pp. 1113-6, 2003

Mullington, Chan, Van Dongen, Szuba, Samaras, Price, Meier-Ewert, Dinges, Mantzoros: "Sleep loss reduces diurnal rhythm amplitude of leptin in healthy men." in: Journal of neuroendocrinology, Vol. 15, Issue 9, pp. 851-4, 2003

Catalano, Marsico, Giordano, Mauro, Rizza, Panno, Andò: "Leptin enhances, via AP-1, expression of aromatase in the MCF-7 cell line." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 28668-76, 2003

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling References for anti-Leptin Antibody (ABIN358628) back to top
Supplier Images
 image for anti-Leptin antibody (LEP) (C-Term) (ABIN358628) anti-Leptin (LEP) (AA 138-167), (C-Term) antibody
 image for anti-Leptin antibody (LEP) (C-Term) (ABIN358628) anti-Leptin (LEP) (AA 138-167), (C-Term) antibody (Image 2)