anti-Human Leptin antibody for ELISA

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
AA 22-168
31
24
18
15
15
15
11
6
6
6
6
6
6
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
420
175
154
24
7
6
5
4
4
4
3
3
2
2
1
1
1
1
1
1
Host
Mouse
373
208
19
8
4
1
Clonality
Monoclonal
Conjugate
This Leptin antibody is un-conjugated
64
26
17
14
10
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
ELISA, Western Blotting (WB)
410
402
108
57
56
39
31
30
29
26
17
13
10
6
5
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN396074
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10.090076 ABIN396826 ELISA Mouse IgG1 kappa AA 22-168 Log in to see M1
10.090076 ABIN561656 ELISA WB Mouse IgG2b kappa AA 22-167, full length Log in to see 3G1-1C9
10.090076 ABIN561657 ELISA Mouse IgG1 kappa AA 22-167, full length Log in to see M1
1 ABIN1996123 ELISA IF IF/ICC WB Mouse IgG1 AA 22-167 Log in to see 8B1F2D2 1
1 ABIN2475318 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 1
1 ABIN214037 IHC (p) ELISA WB Rabbit Log in to see Polyclonal
1 ABIN1499134 IHC (p) ELISA WB Mouse kappa, IgG1 Log in to see
1 ABIN235755 PrA ELISA Mouse IgG1 Log in to see 4F12
1 ABIN235754 PrA ELISA WB Mouse IgG1 Log in to see 3G7
1 ABIN1585862 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1996127 IHC (p) ELISA Rabbit IgG AA 22-167 Log in to see Polyclonal
1 ABIN2893557 ELISA Biotin Mouse IgG1, kappa Log in to see 13A127
1 ABIN1859647 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 22-167 Log in to see
1 ABIN2469665 IHC ELISA WB Rabbit Log in to see Polyclonal
1 ABIN465529 IHC (p) ELISA WB Rabbit Log in to see Polyclonal
1 ABIN108520 IP IHC ELISA WB Rabbit Log in to see Polyclonal 5
1 ABIN1583099 IHC ELISA Mouse IgG1 kappa Log in to see
1 ABIN4901884 ELISA WB Mouse IgG1 Log in to see 7E3-D11-B7
1 ABIN4901883 ELISA WB Mouse IgG2a Log in to see 5C11-G6-B12
1 ABIN299026 ELISA Mouse IgG1 Log in to see 5F10

General

Antigen Leptin (LEP) Antibodies
Epitope AA 22-168
(31), (24), (18), (15), (15), (15), (11), (6), (6), (6), (6), (6), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(420), (175), (154), (24), (7), (6), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(373), (208), (19), (8), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Leptin antibody is un-conjugated
(64), (26), (17), (14), (10), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application ELISA, Western Blotting (WB)
(410), (402), (108), (57), (56), (39), (31), (30), (29), (26), (17), (13), (10), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen LEP (AAH60830, 22 a.a. ~ 168 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3G1-1C9
Isotype IgG2b kappa

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Alternative Name LEP (LEP Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 18641 DA
Gene ID 12406
Research Area Cell Signaling, Cell Structure
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
ELISA image for anti-Leptin (LEP) (AA 22-168) antibody (ABIN396074) Detection limit for recombinant GST tagged LEP is approximately 0.1 ng/mL as a captur...