anti-Human Leptin antibody for Flow Cytometry

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
AA 7-37, N-Term
31
24
17
15
15
15
11
6
6
6
6
6
6
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
420
175
154
24
7
6
5
4
4
4
3
3
2
2
1
1
1
1
1
1
Host
Rabbit
372
209
19
8
4
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
64
26
17
14
10
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
410
403
108
56
55
39
30
30
29
25
17
13
10
6
5
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN953161
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.117655 ABIN1539561 FACS IF IHC (p) WB Rabbit Ig AA 8-37, N-Term Log in to see Polyclonal
14.117655 ABIN5507709 FACS Rabbit IgG AA 22-167 Log in to see 002
1 ABIN3031600 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 8-37 Log in to see Polyclonal
1 ABIN3021884 FACS IF IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1909642 ELISA FACS ICC IF IHC WB APC Rabbit IgG AA 8-37 Log in to see Polyclonal
1 ABIN1909644 ELISA FACS ICC IF IHC WB PE Rabbit IgG AA 8-37 Log in to see Polyclonal
1 ABIN1909645 ELISA FACS ICC IF IHC WB HRP Rabbit IgG AA 8-37 Log in to see Polyclonal
1 ABIN1909643 ELISA FACS ICC IF IHC WB FITC Rabbit IgG AA 8-37 Log in to see Polyclonal
1 ABIN2612685 ELISA FACS IF IHC WB Rabbit IgG AA 7-37 Log in to see Polyclonal
1 ABIN2299456 FACS IF IHC ELISA WB PE Rabbit IgG AA 8-37, N-Term Log in to see Polyclonal
1 ABIN2299433 FACS IF IHC ELISA WB Rabbit IgG AA 8-37, N-Term Log in to see Polyclonal
1 ABIN2299448 FACS IF IHC ELISA WB APC Rabbit IgG AA 8-37, N-Term Log in to see Polyclonal
1 ABIN2299452 FACS IF IHC ELISA WB FITC Rabbit IgG AA 8-37, N-Term Log in to see Polyclonal
1 ABIN5077810 ELISA FACS ICC IF WB Mouse IgG2b kappa AA 22-167 Log in to see 7E10
1 ABIN5077811 ELISA FACS ICC IF WB Mouse IgG2b kappa AA 22-167 Log in to see 7E10
1 ABIN1812489 FACS IF IHC (p) WB Rabbit AA 8-37 Log in to see Polyclonal
1 ABIN5565622 FACS PerCP Rabbit Log in to see Polyclonal
1 ABIN5565620 FACS ICC IF IHC APC Rabbit Log in to see Polyclonal
1 ABIN2299446 FACS IF IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 8-37, N-Term Log in to see Polyclonal
1 ABIN2299450 FACS IF IHC ELISA WB Biotin Rabbit IgG AA 8-37, N-Term Log in to see Polyclonal

General

Antigen Leptin (LEP) Antibodies
Epitope AA 7-37, N-Term
(31), (24), (17), (15), (15), (15), (11), (6), (6), (6), (6), (6), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(420), (175), (154), (24), (7), (6), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(372), (209), (19), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(64), (26), (17), (14), (10), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(410), (403), (108), (56), (55), (39), (30), (30), (29), (25), (17), (13), (10), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling References for anti-Leptin antibody (ABIN953161) Images
Specificity This antibody recognizes Human Leptin (N-term).
Purification Protein A column, followed by peptide affinity purification
Immunogen KLH conjugated synthetic peptide between 7-37 amino acids from the N-terminal region of Human Leptin. Genename: LEP
Isotype Ig

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling References for anti-Leptin antibody (ABIN953161) Images back to top
Antigen
Alternative Name Leptin (LEP Antibody Abstract)
Background This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq].Synonyms: LEP, OB, OBS, Obese protein, Obesity factor
Molecular Weight 18641 Da
Gene ID 3952
NCBI Accession NP_000221
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling References for anti-Leptin antibody (ABIN953161) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details References for anti-Leptin antibody (ABIN953161) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Leptin antibody (ABIN953161)

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling Images back to top
Background publications

Friedlander, Li, Fornage, Williams, Lewis, Schreiner, Pletcher, Enquobahrie, Williams, Siscovick: "Candidate molecular pathway genes related to appetite regulatory neural network, adipocyte homeostasis and obesity: results from the CARDIA Study." in: Annals of human genetics, Vol. 74, Issue 5, pp. 387-98, 2010

Iciek, Wender-Ozegowska, Zawiejska, Seremak-Mrozikiewicz, Drews, Brazert: "[The influence of metabolic parameters on fetal development weight in women with type 1 diabetes and homozygotic variant in -2548 G/A of leptin gene and its 668 A/G receptor polymorphism]." in: Ginekologia polska, Vol. 81, Issue 8, pp. 571-7, 2010

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling References for anti-Leptin antibody (ABIN953161) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Leptin (LEP) (AA 7-37), (N-Term) antibody (ABIN953161) Flow cytometric analysis of Hela cells using Leptin Antibody (N-term) Cat.-No AP52465...
Immunofluorescence (IF) image for anti-Leptin (LEP) (AA 7-37), (N-Term) antibody (ABIN953161) Confocal immunofluorescent analysis of Leptin Antibody (N-term) Cat.-No AP52465PU-N w...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Leptin (LEP) (AA 7-37), (N-Term) antibody (ABIN953161) Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta ...
Western Blotting (WB) image for anti-Leptin (LEP) (AA 7-37), (N-Term) antibody (ABIN953161) Western blot analysis of LEP (arrow) using Leptin Antibody (N-term) Cat.-No AP52465PU...
Western Blotting (WB) image for anti-Leptin (LEP) (AA 7-37), (N-Term) antibody (ABIN953161) Western blot analysis of Leptin Antibody (N-term) in Hela cell line lysates (35ug/lan...