anti-Human Leptin antibody for Immunofluorescence (fixed cells)

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
AA 22-167
30
24
18
15
15
15
11
6
6
6
6
6
6
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
419
173
153
24
7
6
5
4
4
4
3
3
2
2
1
1
1
1
1
1
Host
Mouse
370
209
19
8
4
1
Clonality
Monoclonal
Conjugate
This Leptin antibody is un-conjugated
64
26
17
14
10
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
ELISA, Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC), Western Blotting (WB)
408
402
107
57
55
39
31
30
29
26
17
13
10
5
5
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN1996123
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3002525 IF/ICC Rabbit IgG AA 134-166, C-Term Log in to see Polyclonal

General

Antigen Leptin (LEP) Antibodies
Epitope AA 22-167
(30), (24), (18), (15), (15), (15), (11), (6), (6), (6), (6), (6), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(419), (173), (153), (24), (7), (6), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(370), (209), (19), (8), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Leptin antibody is un-conjugated
(64), (26), (17), (14), (10), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application ELISA, Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC), Western Blotting (WB)
(408), (402), (107), (57), (55), (39), (31), (30), (29), (26), (17), (13), (10), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling ProductDetails: References for anti-Leptin antibody (ABIN1996123) Images
Specificity Human Leptin / LEP
No cross-reactivity with Human Leptin receptor and E.coli lysate in ELISA
No Cross-Reactivity Human, Escherichia coli (E. coli)
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Leptin (rh Leptin, ABIN2215861 NP_000221.1, Val 22-Cys 167,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Human Leptin protein (ABIN2215861)
Clone 8B1F2D2
Isotype IgG1

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling ProductDetails: References for anti-Leptin antibody (ABIN1996123) Images back to top
Antigen
Alternative Name LEP (LEP Antibody Abstract)
Background Human Leptin is one of the most important adipose-derived hormones, and plays a key role in body weight regulation by governing food intake and energy homeostasis. Leptin is produced and secreted mainly by adipocytes, stomach and placenta, and its concentration is affected by several physiological factors such as age, sex, puberty onset and food intake. Leptin exerts actions through its receptor LepRb present in a number of hypothalamic nuclei, partly involved in the satiety signal pathway, and thus inhibit food intake and/or regulate energy expenditure. In addition, leptin is also identified as a new metabolic and neuroendocrine mediator, and functions in a variety of biological processes including glucose metabolism, immune and inflammatory responses, bone mass regulation, as well as normal sexual maturation and reproduction. Leptin deficiency is implicated in severe obesity, morbid obesity with hypogonadism and impaired cell-mediated immunity.
NCBI Accession NP_000221.1
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling ProductDetails: References for anti-Leptin antibody (ABIN1996123) Images back to top
Application Notes WB: 10-20 μg/mL
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human Leptin. The detection limit for Human Leptin is 0.078 ng/well.
ICC/IF: 10-25 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details ProductDetails: References for anti-Leptin antibody (ABIN1996123) Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

ProductDetails: References for anti-Leptin antibody (ABIN1996123)

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling Images back to top
Product cited in:

Sun, Park, Gupta, Holland, Auerbach, Zhang, Goncalves Marangoni, Nicoloro, Czech, Varga, Ploug, An, Scherer: "Endotrophin triggers adipose tissue fibrosis and metabolic dysfunction." in: Nature communications, Vol. 5, pp. 3485, 2014

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling ProductDetails: References for anti-Leptin antibody (ABIN1996123) back to top
Supplier Images
 image for anti-Leptin (LEP) (AA 22-167) antibody (ABIN1996123) anti-Leptin (LEP) (AA 22-167) antibody