anti-Human Leptin antibody for Immunohistochemistry

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
Middle Region
31
24
18
15
15
15
11
6
6
6
6
6
6
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Goat, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
416
171
151
23
6
5
4
4
3
3
3
2
2
2
1
1
1
1
1
1
Host
Rabbit
367
207
19
8
4
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
64
26
17
14
10
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
406
400
106
57
56
39
31
30
29
26
17
10
10
6
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN2776944
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.637687 ABIN2776943 IHC WB Rabbit N-Term Log in to see Polyclonal 1
9.637687 ABIN152764 ICC IHC IHC (fro) WB Rabbit IgG Log in to see Polyclonal
9.637687 ABIN2469665 IHC ELISA WB Rabbit Log in to see Polyclonal
9.637687 ABIN4904218 IHC WB Rabbit IgG Log in to see Polyclonal
9.637687 ABIN3031600 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 8-37 Log in to see Polyclonal
9.637687 ABIN2421035 IHC ELISA Rabbit IgG Log in to see Polyclonal
9.637687 ABIN5547256 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN1585862 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1876874 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1078271 ICC IHC IP WB Rabbit IgG AA 22-167 Log in to see Polyclonal
1 ABIN108520 IP IHC ELISA WB Rabbit Log in to see Polyclonal 5
1 ABIN1583099 IHC ELISA Mouse IgG1 kappa Log in to see
1 ABIN4330701 ELISA IHC IHC (p) Mouse IgG1 kappa Log in to see 3
1 ABIN3021884 FACS IF IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2612683 ELISA IHC WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN2612684 ELISA IHC WB FITC Rabbit IgG Log in to see Polyclonal
1 ABIN2612682 ELISA IHC WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN2884678 IHC ELISA Rabbit IgG AA 36-85 Log in to see Polyclonal
1 ABIN1584698 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1822624 IF IHC Rabbit IgG AA 57-92 Log in to see Polyclonal

Top referenced anti-Leptin antibody for Immunohistochemistry

Similar anti-Leptin Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Horse (Equine) Cow (Bovine) Sheep (Ovine) Pig (Porcine) Dog (Canine) Rabbit Goat
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 107 Antibodies 264 Antibodies 90 Antibodies 6 Antibodies 5 Antibodies 12 Antibodies 7 Antibodies 4 Antibodies 3 Antibodies 4 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Radioimmunoassay (RIA) 6 Antibodies 3 Antibodies 3 Antibodies 1 Antibodies

General

Antigen Leptin (LEP) Antibodies
Epitope Middle Region
(31), (24), (18), (15), (15), (15), (11), (6), (6), (6), (6), (6), (6), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Goat, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(416), (171), (151), (23), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(367), (207), (19), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(64), (26), (17), (14), (10), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(406), (400), (106), (57), (56), (39), (31), (30), (29), (26), (17), (10), (10), (6), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN979273
Sequence LHVLAFSKSC HLPWASGLET LDSLGGVLEA SGYSTEVVAL SRLQGSLQDM
Predicted Reactivity Cow: 100%, Dog: 86%, Goat: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 93%, Rat: 100%, Sheep: 100%
Characteristics This is a rabbit polyclonal antibody against LEP. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human LEP

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Alternative Name LEP (LEP Antibody Abstract)
Background LEP is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: OB, OBS
Protein Interaction Partner: Hk3, SIGLEC6, GHRL, LEPR, UCN, PRKAA2, CLU, CRP, A2M, STAT3,
Protein Size: 167
Molecular Weight 16 kDa
Gene ID 3952
NCBI Accession NM_000230, NP_000221
UniProt P41159
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 167 AA

Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Leptin antibody (LEP) (Middle Region) (ABIN2776944) WB Suggested Anti-LEP Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1...
 image for anti-Leptin antibody (LEP) (Middle Region) (ABIN2776944) anti-Leptin (LEP) (Middle Region) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Leptin antibody (LEP) (Middle Region) (ABIN2776944) anti-Leptin (LEP) (Middle Region) antibody (Image 3)