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anti-Human Leptin antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • LEPD
  • OB
  • ob
  • obese
  • OBS
Epitope
AA 131-145
24
22
21
13
13
11
9
8
6
6
6
6
5
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
331
151
131
18
3
1
1
Host
Mouse
349
133
20
8
4
Clonality
Monoclonal
Conjugate
This Leptin antibody is un-conjugated
60
19
13
7
7
5
5
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
337
321
69
39
39
33
26
25
17
17
16
13
10
7
5
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN235759
$ 544.13
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008676689 ABIN235762 IHC (f) Mouse IgG2a AA 92-145, cyclized Log in to see BDI142

Similar anti-Leptin Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 3 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 229 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 3 Antibodies
Enzyme Immunoassay (EIA) 13 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunoassay (IA) 7 Antibodies
Immunochromatography (IC) 15 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 39 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 57 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies
Immunoprecipitation (IP) 19 Antibodies
Immunostaining (ISt) 1 Antibodies

General

Antigen Leptin (LEP) Antibodies
Epitope AA 131-145
(24), (22), (21), (13), (13), (11), (9), (8), (6), (6), (6), (6), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(331), (151), (131), (18), (3), (1), (1)
Host Mouse
(349), (133), (20), (8), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Leptin antibody is un-conjugated
(60), (19), (13), (7), (7), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
(337), (321), (69), (39), (39), (33), (26), (25), (17), (17), (16), (13), (10), (7), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Specificity Human Leptin. Binds to the carboxyl terminal region of the protein and to the intact leptin specifically in ELISA, Western blot and immunocytochemistry.
Cross-Reactivity (Details) Cross-reactivity: Leptin (Human) 100 % Leptin (131-145) 100 % Epidermal Growth Factor (Human) 0 % Insulin (Human) 0 % Insulin-like Growth Factor 1 (Human) 0 % Insulin-like Growth Factor 2 (Rat) 0 % Parathyroid hormone (Human) 0 % Transforming Growth Factor-alpha (Human) 0 %
Characteristics MAb to Leptin, Monoclonal antibody to Human Leptin, (AA 131-145)
Purification Ascites
Immunogen Synthetic human leptin (131-145) attached to a carrier protein
Clone BDI170
Isotype IgG2a

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Leptin (LEP Antibody Abstract)
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes Has been found to stain specifically human adipocytes at a 1:1000 dilution. The ability to bind to leptin in adipocytes was examined in cells fixed with neutral buffered formalin. The fixed cells were incubated for 20 minutes with 4 % normal goat serum, reacted for 60 minutes with the diluted monoclonal antibody and then with FITC-conjugated Goat anti-Mouse IgG. The immunofluorescent staining pattern was observed using epifluorescence microscopy. Western immunoblots of adipocyte homogenates resulted in a single band being detected at ~16 kDa at a dilution of 1:4000. Suitable for use in IHC using frozen sections. Paraffin-embedded sections have not been tested.
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Liquid
Preservative Without preservative
Handling Advice Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
Storage -20 °C
Storage Comment Upon receipt, store at -20 °C.