anti-Human Leptin antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
AA 92-145, cyclized
31
24
18
15
15
15
10
6
6
6
6
6
6
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
420
175
154
24
7
6
5
4
4
4
3
3
2
2
1
1
1
1
1
1
Host
Mouse
373
208
19
8
4
1
Clonality
Monoclonal
Conjugate
This Leptin antibody is un-conjugated
64
26
17
14
10
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
411
403
108
57
56
39
31
30
29
26
17
13
10
6
5
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN235762
$ 582.27
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN235759 IHC (f) WB Mouse IgG2a AA 131-145 Log in to see BDI170

General

Antigen Leptin (LEP) Antibodies
Epitope AA 92-145, cyclized
(31), (24), (18), (15), (15), (15), (10), (6), (6), (6), (6), (6), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(420), (175), (154), (24), (7), (6), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(373), (208), (19), (8), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Leptin antibody is un-conjugated
(64), (26), (17), (14), (10), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(411), (403), (108), (57), (56), (39), (31), (30), (29), (26), (17), (13), (10), (6), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Specificity Human Leptin. Binds to the carboxyl terminal region of the protein and to the intact leptin specifically in ELISA and immunocytochemistry.
Cross-Reactivity (Details) Cross-reactivity: Leptin (Human) 100 % Leptin (92-145) cyclized 100 % Epidermal Growth Factor (Human) 0 % Insulin (Human) 0 % Insulin-like Growth Factor 1 (Human) 0 % Insulin-like Growth Factor 2 (Rat) 0 % Parathyroid hormone (Human) 0 % Transforming Growth Factor-alpha (Human) 0 %
Characteristics MAb to Leptin, Monoclonal antibody to Human Leptin (92-145) cyclized
Purification Ascites
Immunogen Synthetic human leptin (92-145) cyclized
Clone BDI142
Isotype IgG2a

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Leptin (LEP Antibody Abstract)
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes Has been found to stain specifically human adipocytes at a 1:2.000 dilution. The ability to bind to leptin in adipocytes was examined in cells fixed with neutral buffered formalin. The fixed cells were incubated for 20 minutes with 4 % normal goat serum, reacted for 60 minutes with the diluted monoclonal antibody and then with FITC-conjugated Goat anti-Mouse IgG. The immunofluorescent staining pattern was observed using epifluorescence microscopy. Suitable for use in IHC using frozen sections. Paraffin-embedded sections have not been tested.
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Liquid
Preservative Without preservative
Handling Advice Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
Storage -20 °C
Storage Comment Upon receipt, store at -20 °C.