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anti-Human Leptin antibody for Immunohistochemistry (Frozen Sections)

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • LEPD
  • OB
  • ob
  • obese
  • OBS
Reactivity
Human
446
153
133
18
3
2
2
1
Host
Rabbit
350
247
20
8
4
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
66
25
19
13
9
9
7
6
6
6
6
6
6
6
6
6
6
6
6
5
5
3
3
3
3
3
3
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
449
415
72
59
41
33
25
24
19
17
16
13
10
7
5
3
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN115495
$ 478.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000883884 ABIN111426 IHC (fro) IF WB Mouse IgG2a AA 131-145, C-Term Log in to see BDI170
0.000883884 ABIN111428 IHC (fro) IF WB Mouse IgG2a AA 92-145, cyclized Log in to see BDI142
0.000883884 ABIN152764 ICC IHC (fro) IF IHC WB Rabbit Log in to see Polyclonal
0.000883884 ABIN1859647 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 22-167 Log in to see
0.000883884 ABIN1078271 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-167 Log in to see Polyclonal

Top referenced anti-Leptin antibody for Immunohistochemistry (Frozen Sections)

Similar anti-Leptin Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 3 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 338 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 3 Antibodies
Enzyme Immunoassay (EIA) 13 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunoassay (IA) 7 Antibodies
Immunochromatography (IC) 15 Antibodies
Immunocytochemistry (ICC) 11 Antibodies
Immunofluorescence (IF) 40 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 59 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 44 Antibodies
Immunoprecipitation (IP) 19 Antibodies

General

Antigen Leptin (LEP) Antibodies
Reactivity Human
(446), (153), (133), (18), (3), (2), (2), (1)
Host Rabbit
(350), (247), (20), (8), (4)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(66), (25), (19), (13), (9), (9), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(449), (415), (72), (59), (41), (33), (25), (24), (19), (17), (16), (13), (10), (7), (5), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling References for anti-Leptin Antibody (ABIN115495) Images
Specificity The antibody was raised in rabbits by immunization with the recombinant Human Leptin.
Purification Immunoaffinity chromatography
Immunogen Recombinant human Leptin (E. coli).

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling References for anti-Leptin Antibody (ABIN115495) Images back to top
Antigen
Alternative Name Leptin (LEP Antibody Abstract)
Background Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action, it decreases the appetite and increases energy consumption, causing more fat to be burned.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor
Gene ID 3952
NCBI Accession NP_000221
UniProt P41159
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling References for anti-Leptin Antibody (ABIN115495) Images back to top
Application Notes ELISA. Immunohistochemistry. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details References for anti-Leptin Antibody (ABIN115495) Images back to top
Reconstitution Restore with 0.1 mL of deionized water
Concentration 1.0 mg/mL
Buffer 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
Expiry Date 6 months

References for anti-Leptin Antibody (ABIN115495)

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling Images back to top
Background publications

Ricci, Lee, Russell, Wang, Sullivan, Schneider, Brolin, Fried: "Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms." in: American journal of physiology. Endocrinology and metabolism, Vol. 288, Issue 4, pp. E798-804, 2005

Schubring, Prohaska, Prohaska, Englaro, Blum, Siebler, Kratzsch, Kiess: "Leptin concentrations in maternal serum and amniotic fluid during the second trimenon: differential relation to fetal gender and maternal morphometry." in: European journal of obstetrics, gynecology, and reproductive biology, Vol. 86, Issue 2, pp. 151-7, 1999

Considine, Sinha, Heiman, Kriauciunas, Stephens, Nyce, Ohannesian, Marco, McKee, Bauer: "Serum immunoreactive-leptin concentrations in normal-weight and obese humans." in: The New England journal of medicine, Vol. 334, Issue 5, pp. 292-5, 1996

Maffei, Halaas, Ravussin, Pratley, Lee, Zhang, Fei, Kim, Lallone, Ranganathan: "Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects." in: Nature medicine, Vol. 1, Issue 11, pp. 1155-61, 1995

Lönnqvist, Arner, Nordfors, Schalling: "Overexpression of the obese (ob) gene in adipose tissue of human obese subjects." in: Nature medicine, Vol. 1, Issue 9, pp. 950-3, 1995