anti-Human Leptin antibody for Immunohistochemistry (Frozen Sections)

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Reactivity
Bird (Avian), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
419
172
152
24
7
5
4
4
4
4
3
3
2
2
1
1
1
1
1
Host
Rabbit
369
210
19
8
4
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
64
26
17
14
10
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
408
403
106
57
56
39
31
30
28
26
17
13
9
6
5
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN152764
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN115495 EIA IHC (fro) IP WB Rabbit Log in to see Polyclonal 5
1 ABIN111426 IHC (fro) IF WB Mouse IgG2a AA 131-145, C-Term Log in to see BDI170
1 ABIN111428 IHC (fro) IF WB Mouse IgG2a AA 92-145, cyclized Log in to see BDI142
1 ABIN1859647 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 22-167 Log in to see
1 ABIN2742564 ICC IHC (fro) WB Rabbit IgG AA 25-44 Log in to see Polyclonal

General

Antigen Leptin (LEP) Antibodies
Reactivity Bird (Avian), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(419), (172), (152), (24), (7), (5), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(369), (210), (19), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(64), (26), (17), (14), (10), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(408), (403), (106), (57), (56), (39), (31), (30), (28), (26), (17), (13), (9), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Specificity Leptin
Purification Immunogen affinity purified
Immunogen Synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin.
Isotype IgG

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Leptin/OB (LEP Antibody Abstract)
Background Gene Symbol: LEP
Gene ID 3952
UniProt P41160
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes Western Blot 1:200 - 1:1000, Immunohistochemistry 1:50 - 1:200, Immunohistochemistry-Frozen 1:50 - 1:200, Immunocytochemistry 1:50 - 1:200WB: Detects an approx. 16 kDa protein representing leptin from 3T3-L1 cell extract. IHC: Staining of leptin in sheep brain with NB300-611 results in staining of the ventromedial hypothalamus.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Leptin Antibody Western Blot Protocol
1. Block with blotto 1 HR at RT
. Dilute primary 1:2000 - 1:4000 in blotto for 1 HR at RT
. Wash
. Secondary GAR-AP 1:2000 (or HRP) but dilution for secondary should be specific to detection method being used
. Wash
. Detect
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS and 1 mg/mL BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Leptin (LEP) antibody (ABIN152764) anti-Leptin (LEP) antibody
Immunohistochemistry (IHC) image for anti-Leptin (LEP) antibody (ABIN152764) Figure 2 shows staining of Leptin in sheep brain using ABIN152764.