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anti-Human Leptin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
AA 7-37, N-Term
24
22
21
13
13
11
10
7
6
6
6
6
6
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
414
154
132
18
3
2
2
1
Host
Rabbit
354
212
20
8
4
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
65
23
17
11
7
7
7
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
413
386
87
59
39
33
25
25
19
17
15
14
10
7
5
3
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN953161
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.203815 ABIN498515 IHC (p) Rabbit Log in to see Polyclonal
11.203815 ABIN668243 IF (p) IHC (p) WB Rabbit IgG AA 1-50 Log in to see Polyclonal
11.203815 ABIN4330701 ELISA IHC IHC (p) Mouse IgG1 kappa Log in to see 3
11.203815 ABIN4892220 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN604738 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1539561 FACS IF IHC (p) WB Rabbit Ig AA 8-37, N-Term Log in to see Polyclonal
1 ABIN604739 IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN1499134 IHC (p) ELISA WB Mouse kappa, IgG1 Log in to see
1 ABIN1859647 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 22-167 Log in to see
1 ABIN1078271 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-167 Log in to see Polyclonal
1 ABIN1996127 IHC (p) ELISA Rabbit IgG AA 22-167 Log in to see Polyclonal
1 ABIN668252 IHC (p) WB HRP Rabbit IgG AA 1-50 Log in to see Polyclonal
1 ABIN465529 IHC (p) ELISA WB Rabbit Log in to see Polyclonal
1 ABIN302917 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN668245 IHC (p) WB Biotin Rabbit IgG AA 1-50 Log in to see Polyclonal
1 ABIN1812489 FACS IF IHC (p) WB Rabbit AA 7-37 Log in to see Polyclonal
1 ABIN1736401 IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN707495 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN707488 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN707486 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Leptin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-Leptin Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Peptide (BP) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 301 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 3 Antibodies
Enzyme Immunoassay (EIA) 13 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunoassay (IA) 7 Antibodies
Immunochromatography (IC) 15 Antibodies
Immunocytochemistry (ICC) 11 Antibodies
Immunofluorescence (IF) 39 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 74 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 45 Antibodies
Immunoprecipitation (IP) 19 Antibodies

General

Antigen Leptin (LEP) Antibodies
Epitope AA 7-37, N-Term
(24), (22), (21), (13), (13), (11), (10), (7), (6), (6), (6), (6), (6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(414), (154), (132), (18), (3), (2), (2), (1)
Host Rabbit
(354), (212), (20), (8), (4)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(65), (23), (17), (11), (7), (7), (7), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(413), (386), (87), (59), (39), (33), (25), (25), (19), (17), (15), (14), (10), (7), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling References for anti-Leptin Antibody (ABIN953161) Images
Specificity This antibody recognizes Human Leptin (N-term).
Purification Protein A column, followed by peptide affinity purification
Immunogen KLH conjugated synthetic peptide between 7-37 amino acids from the N-terminal region of Human Leptin. Genename: LEP
Isotype Ig

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling References for anti-Leptin Antibody (ABIN953161) Images back to top
Antigen
Alternative Name Leptin (LEP Antibody Abstract)
Background This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq].Synonyms: LEP, OB, OBS, Obese protein, Obesity factor
Molecular Weight 18641 Da
Gene ID 3952
NCBI Accession NP_000221
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling References for anti-Leptin Antibody (ABIN953161) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details References for anti-Leptin Antibody (ABIN953161) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Leptin Antibody (ABIN953161)

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling Images back to top
Background publications

Friedlander, Li, Fornage, Williams, Lewis, Schreiner, Pletcher, Enquobahrie, Williams, Siscovick: "Candidate molecular pathway genes related to appetite regulatory neural network, adipocyte homeostasis and obesity: results from the CARDIA Study." in: Annals of human genetics, Vol. 74, Issue 5, pp. 387-98, 2010

Iciek, Wender-Ozegowska, Zawiejska, Seremak-Mrozikiewicz, Drews, Brazert: "[The influence of metabolic parameters on fetal development weight in women with type 1 diabetes and homozygotic variant in -2548 G/A of leptin gene and its 668 A/G receptor polymorphism]." in: Ginekologia polska, Vol. 81, Issue 8, pp. 571-7, 2010

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling References for anti-Leptin Antibody (ABIN953161) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Leptin antibody (LEP) (AA 7-37) (ABIN953161) Flow cytometric analysis of Hela cells using Leptin Antibody (N-term) Cat.-No AP52465...
Immunofluorescence (IF) image for anti-Leptin antibody (LEP) (AA 7-37) (ABIN953161) Confocal immunofluorescent analysis of Leptin Antibody (N-term) Cat.-No AP52465PU-N w...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Leptin antibody (LEP) (AA 7-37) (ABIN953161) Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta ...
Western Blotting (WB) image for anti-Leptin antibody (LEP) (AA 7-37) (ABIN953161) Western blot analysis of LEP (arrow) using Leptin Antibody (N-term) Cat.-No AP52465PU...
Western Blotting (WB) image for anti-Leptin antibody (LEP) (AA 7-37) (ABIN953161) Western blot analysis of Leptin Antibody (N-term) in Hela cell line lysates (35ug/lan...