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anti-Human Leptin antibody for Immunoprecipitation

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • LEPD
  • OB
  • ob
  • obese
  • OBS
Reactivity
Human
440
151
131
18
3
1
1
Host
Rabbit
348
243
20
8
4
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
66
25
19
13
9
9
7
6
6
6
6
6
6
6
6
6
6
6
6
5
5
3
3
3
3
3
3
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
445
410
69
57
39
33
25
24
17
17
16
12
10
7
5
3
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN115495
$ 478.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008908373 ABIN108520 IP IHC ELISA WB Rabbit Log in to see Polyclonal 5
0.0008908373 ABIN1169155 IP ELISA WB Biotin Rabbit Log in to see Polyclonal
0.0008908373 ABIN226993 IP ELISA WB Mouse IgG1 Log in to see YD-HLEP4B6
0.0008908373 ABIN1169152 IP ELISA WB Rabbit Log in to see Polyclonal
0.0008908373 ABIN221398 IP RIA ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0008908373 ABIN1002719 IP ELISA WB Mouse IgG1 Log in to see YD-HLEP4B6
0.0008908373 ABIN2299697 IP ELISA WB Mouse IgG1 Log in to see 5C73
0.0008908373 ABIN2299506 IP ELISA WB Mouse IgG Log in to see 13F226
0.0008908373 ABIN2145801 IP IHC ELISA WB Mouse IgG1 Log in to see YD-HLEP4B6
0.0008908373 ABIN2299723 IP RIA ELISA WB Rabbit IgG AA 1-24 Log in to see Polyclonal
0.0008908373 ABIN2963302 IP ELISA WB Biotin Rabbit Log in to see Polyclonal
0.0008908373 ABIN2299650 IP ELISA WB Biotin Rabbit Log in to see Polyclonal
0.0008908373 ABIN2489210 IP ELISA WB Mouse IgG1 Log in to see YD-HLEP4B6-
0.0008908373 ABIN2963301 IP ELISA WB Rabbit Log in to see Polyclonal
0.0008908373 ABIN2299645 IP ELISA WB Rabbit Log in to see Polyclonal
0.0008908373 ABIN2299725 IP RIA ELISA WB Rabbit Log in to see Polyclonal
0.0008908373 ABIN808215 IP TCC ELISA Mouse Log in to see YD-HLEP4B6
0.0008908373 ABIN645761 IP ELISA WB Mouse IgG1 Log in to see

Top referenced anti-Leptin antibody for Immunoprecipitation

Similar anti-Leptin Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 3 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 337 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 3 Antibodies
Enzyme Immunoassay (EIA) 13 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunoassay (IA) 7 Antibodies
Immunochromatography (IC) 15 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 39 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 57 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies
Immunoprecipitation (IP) 19 Antibodies
Immunostaining (ISt) 1 Antibodies

General

Antigen Leptin (LEP) Antibodies
Reactivity Human
(440), (151), (131), (18), (3), (1), (1)
Host Rabbit
(348), (243), (20), (8), (4)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(66), (25), (19), (13), (9), (9), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(445), (410), (69), (57), (39), (33), (25), (24), (17), (17), (16), (12), (10), (7), (5), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling References for anti-Leptin Antibody (ABIN115495) Images
Specificity The antibody was raised in rabbits by immunization with the recombinant Human Leptin.
Purification Immunoaffinity chromatography
Immunogen Recombinant human Leptin (E. coli).

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling References for anti-Leptin Antibody (ABIN115495) Images back to top
Antigen
Alternative Name Leptin (LEP Antibody Abstract)
Background Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action, it decreases the appetite and increases energy consumption, causing more fat to be burned.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor
Gene ID 3952
NCBI Accession NP_000221
UniProt P41159
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling References for anti-Leptin Antibody (ABIN115495) Images back to top
Application Notes ELISA. Immunohistochemistry. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details References for anti-Leptin Antibody (ABIN115495) Images back to top
Reconstitution Restore with 0.1 mL of deionized water
Concentration 1.0 mg/mL
Buffer 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
Expiry Date 6 months

References for anti-Leptin Antibody (ABIN115495)

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling Images back to top
Background publications

Ricci, Lee, Russell, Wang, Sullivan, Schneider, Brolin, Fried: "Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms." in: American journal of physiology. Endocrinology and metabolism, Vol. 288, Issue 4, pp. E798-804, 2005

Schubring, Prohaska, Prohaska, Englaro, Blum, Siebler, Kratzsch, Kiess: "Leptin concentrations in maternal serum and amniotic fluid during the second trimenon: differential relation to fetal gender and maternal morphometry." in: European journal of obstetrics, gynecology, and reproductive biology, Vol. 86, Issue 2, pp. 151-7, 1999

Considine, Sinha, Heiman, Kriauciunas, Stephens, Nyce, Ohannesian, Marco, McKee, Bauer: "Serum immunoreactive-leptin concentrations in normal-weight and obese humans." in: The New England journal of medicine, Vol. 334, Issue 5, pp. 292-5, 1996

Maffei, Halaas, Ravussin, Pratley, Lee, Zhang, Fei, Kim, Lallone, Ranganathan: "Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects." in: Nature medicine, Vol. 1, Issue 11, pp. 1155-61, 1995

Lönnqvist, Arner, Nordfors, Schalling: "Overexpression of the obese (ob) gene in adipose tissue of human obese subjects." in: Nature medicine, Vol. 1, Issue 9, pp. 950-3, 1995