anti-Human Leptin antibody for Western Blotting

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
AA 7-37, N-Term
31
24
17
15
15
15
11
6
6
6
6
6
6
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
416
172
152
24
7
6
5
4
4
4
3
3
2
2
1
1
1
1
1
1
Host
Rabbit
367
207
19
8
4
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
64
26
17
14
10
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
406
400
107
56
55
39
30
30
29
25
17
10
10
6
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN953161
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3487515 ABIN390092 IF WB Rabbit Ig AA 134-166, C-Term Log in to see Polyclonal 1
3.3487515 ABIN2776944 IHC WB Rabbit Middle Region Log in to see Polyclonal
3.3487515 ABIN1539561 FACS IF IHC (p) WB Rabbit Ig AA 8-37, N-Term Log in to see Polyclonal
3.3487515 ABIN2467855 WB Chicken full length Log in to see Polyclonal
3.3487515 ABIN152764 ICC IHC IHC (fro) WB Rabbit IgG Log in to see Polyclonal
3.3487515 ABIN1859647 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 22-167 Log in to see
3.3487515 ABIN1876874 IHC WB Rabbit IgG Log in to see Polyclonal
3.3487515 ABIN1078271 ICC IHC IP WB Rabbit IgG AA 22-167 Log in to see Polyclonal
3.3487515 ABIN4904218 IHC WB Rabbit IgG Log in to see Polyclonal
3.3487515 ABIN223580 WB Rabbit IgG Log in to see Polyclonal
3.3487515 ABIN3021884 FACS IF IHC WB Rabbit IgG Log in to see Polyclonal
3.3487515 ABIN3031600 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 8-37 Log in to see Polyclonal
3.3487515 ABIN634794 WB Rabbit N-Term Log in to see Polyclonal
3.3487515 ABIN634796 WB Rabbit Middle Region Log in to see Polyclonal
3.3487515 ABIN1169155 IP ELISA WB Biotin Rabbit Log in to see Polyclonal
3.3487515 ABIN3028789 IF ELISA WB Rabbit Ig Fraction AA 134-166 Log in to see Polyclonal
3.3487515 ABIN4892220 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
3.3487515 ABIN4961832 IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal
3.3487515 ABIN2406454 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN358628 EIA IF WB Rabbit Ig C-Term, AA 138-167 Log in to see Polyclonal 4

Top referenced anti-Leptin antibody for Western Blotting

Similar anti-Leptin Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Peptide (BP) 1 Antibodies
Cell Culture (CC) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 291 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 4 Antibodies
Enzyme Immunoassay (EIA) 13 Antibodies
Flow Cytometry (FACS) 23 Antibodies
Immunoassay (IA) 4 Antibodies
Immunochromatography (IC) 15 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 49 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 81 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies

General

Antigen Leptin (LEP) Antibodies
Epitope AA 7-37, N-Term
(31), (24), (17), (15), (15), (15), (11), (6), (6), (6), (6), (6), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(416), (172), (152), (24), (7), (6), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(367), (207), (19), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(64), (26), (17), (14), (10), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(406), (400), (107), (56), (55), (39), (30), (30), (29), (25), (17), (10), (10), (6), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling ProductDetails: References for anti-Leptin antibody (ABIN953161) Images
Specificity This antibody recognizes Human Leptin (N-term).
Purification Protein A column, followed by peptide affinity purification
Immunogen KLH conjugated synthetic peptide between 7-37 amino acids from the N-terminal region of Human Leptin. Genename: LEP
Isotype Ig

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling ProductDetails: References for anti-Leptin antibody (ABIN953161) Images back to top
Antigen
Alternative Name Leptin (LEP Antibody Abstract)
Background This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq].Synonyms: LEP, OB, OBS, Obese protein, Obesity factor
Molecular Weight 18641 Da
Gene ID 3952
NCBI Accession NP_000221
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling ProductDetails: References for anti-Leptin antibody (ABIN953161) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details ProductDetails: References for anti-Leptin antibody (ABIN953161) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-Leptin antibody (ABIN953161)

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling Images back to top
Background publications

Friedlander, Li, Fornage, Williams, Lewis, Schreiner, Pletcher, Enquobahrie, Williams, Siscovick: "Candidate molecular pathway genes related to appetite regulatory neural network, adipocyte homeostasis and obesity: results from the CARDIA Study." in: Annals of human genetics, Vol. 74, Issue 5, pp. 387-98, 2010

Iciek, Wender-Ozegowska, Zawiejska, Seremak-Mrozikiewicz, Drews, Brazert: "[The influence of metabolic parameters on fetal development weight in women with type 1 diabetes and homozygotic variant in -2548 G/A of leptin gene and its 668 A/G receptor polymorphism]." in: Ginekologia polska, Vol. 81, Issue 8, pp. 571-7, 2010

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling ProductDetails: References for anti-Leptin antibody (ABIN953161) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Leptin antibody (LEP) (AA 7-37) (ABIN953161) Flow cytometric analysis of Hela cells using Leptin Antibody (N-term) Cat.-No AP52465...
Immunofluorescence (IF) image for anti-Leptin antibody (LEP) (AA 7-37) (ABIN953161) Confocal immunofluorescent analysis of Leptin Antibody (N-term) Cat.-No AP52465PU-N w...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Leptin antibody (LEP) (AA 7-37) (ABIN953161) Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta ...
Western Blotting (WB) image for anti-Leptin antibody (LEP) (AA 7-37) (ABIN953161) Western blot analysis of LEP (arrow) using Leptin Antibody (N-term) Cat.-No AP52465PU...
Western Blotting (WB) image for anti-Leptin antibody (LEP) (AA 7-37) (ABIN953161) Western blot analysis of Leptin Antibody (N-term) in Hela cell line lysates (35ug/lan...