anti-Human Leptin antibody for Western Blotting

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
Middle Region
31
24
18
15
15
15
11
6
6
6
6
6
6
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Goat, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
418
171
151
24
6
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
Host
Rabbit
367
209
18
8
4
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
64
26
17
14
10
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
408
401
105
56
55
39
31
30
29
26
17
13
10
6
5
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN2776944
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.22672 ABIN953161 EIA FACS IF IHC (p) WB Rabbit Ig AA 7-37, N-Term Log in to see Polyclonal 2
3.22672 ABIN390092 IF WB Rabbit Ig AA 134-166, C-Term Log in to see Polyclonal 1
3.22672 ABIN152764 ICC IHC IHC (fro) WB Rabbit IgG Log in to see Polyclonal
3.22672 ABIN1539561 FACS IF IHC (p) WB Rabbit Ig AA 8-37, N-Term Log in to see Polyclonal
3.22672 ABIN2467855 WB Chicken full length Log in to see Polyclonal
3.22672 ABIN4892220 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
3.22672 ABIN223580 WB Rabbit IgG Log in to see Polyclonal
3.22672 ABIN1876874 IHC WB Rabbit IgG Log in to see Polyclonal
3.22672 ABIN1859647 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 22-167 Log in to see
3.22672 ABIN4904218 IHC WB Rabbit IgG Log in to see Polyclonal
3.22672 ABIN1078271 ICC IHC IP WB Rabbit IgG AA 22-167 Log in to see Polyclonal
3.22672 ABIN634796 WB Rabbit Middle Region Log in to see Polyclonal
3.22672 ABIN634794 WB Rabbit N-Term Log in to see Polyclonal
3.22672 ABIN3031600 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 8-37 Log in to see Polyclonal
3.22672 ABIN3021884 FACS IF IHC WB Rabbit IgG Log in to see Polyclonal
3.22672 ABIN1169155 IP ELISA WB Biotin Rabbit Log in to see Polyclonal
3.22672 ABIN3028789 IF ELISA WB Rabbit Ig Fraction AA 134-166 Log in to see Polyclonal
3.22672 ABIN4961832 IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal
3.22672 ABIN2406454 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2776943 IHC WB Rabbit N-Term Log in to see Polyclonal 1

General

Antigen Leptin (LEP) Antibodies
Epitope Middle Region
(31), (24), (18), (15), (15), (15), (11), (6), (6), (6), (6), (6), (6), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Goat, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(418), (171), (151), (24), (6), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(367), (209), (18), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(64), (26), (17), (14), (10), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(408), (401), (105), (56), (55), (39), (31), (30), (29), (26), (17), (13), (10), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN979273
Sequence LHVLAFSKSC HLPWASGLET LDSLGGVLEA SGYSTEVVAL SRLQGSLQDM
Predicted Reactivity Cow: 100%, Dog: 86%, Goat: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 93%, Rat: 100%, Sheep: 100%
Characteristics This is a rabbit polyclonal antibody against LEP. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human LEP

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Alternative Name LEP (LEP Antibody Abstract)
Background LEP is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: OB, OBS
Protein Interaction Partner: Hk3, SIGLEC6, GHRL, LEPR, UCN, PRKAA2, CLU, CRP, A2M, STAT3,
Protein Size: 167
Molecular Weight 16 kDa
Gene ID 3952
NCBI Accession NM_000230, NP_000221
UniProt P41159
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 167 AA

Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Leptin (LEP) (Middle Region) antibody (ABIN2776944) WB Suggested Anti-LEP Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1...
 image for anti-Leptin (LEP) (Middle Region) antibody (ABIN2776944) anti-Leptin (LEP) (Middle Region) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Leptin (LEP) (Middle Region) antibody (ABIN2776944) anti-Leptin (LEP) (Middle Region) antibody (Image 3)