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anti-Mouse (Murine) Leptin antibody for ELISA

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
AA 74-109, Middle Region
27
24
19
15
15
15
11
6
6
6
6
6
5
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
424
170
145
22
6
4
4
4
3
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
359
215
20
8
4
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
65
23
17
11
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
ELISA, Western Blotting (WB)
418
415
100
56
48
39
34
26
21
19
17
10
10
7
5
5
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN3043294
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1585862 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN214037 IHC (p) ELISA WB Rabbit Log in to see Polyclonal
1 ABIN465756 ELISA IHC (pfa) WB Biotin Rabbit Log in to see Polyclonal
1 ABIN1169154 IP ELISA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN3201294 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-167 Log in to see
1 ABIN103522 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN108522 ELISA WB Rabbit Log in to see Polyclonal 6
1 ABIN108521 ELISA WB Rabbit Log in to see Polyclonal 4
1 ABIN2469665 IHC ELISA WB Rabbit Log in to see Polyclonal
1 ABIN465757 IHC (pfa) ELISA WB Rabbit Log in to see Polyclonal
1 ABIN1169151 IP ELISA WB Rabbit Log in to see Polyclonal
1 ABIN205695 IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN103521 ELISA WB Rabbit Log in to see Polyclonal
1 ABIN1736401 IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1584698 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2884678 IHC ELISA Rabbit IgG AA 36-85 Log in to see Polyclonal
1 ABIN2612674 ELISA WB Biotin Rabbit IgG AA 22-167 Log in to see Polyclonal
1 ABIN2612677 ELISA WB FITC Rabbit IgG AA 22-167 Log in to see Polyclonal
1 ABIN2475320 ELISA Sheep IgG AA 57-70 Log in to see Polyclonal 2
1 ABIN799626 ELISA WB Rabbit Log in to see Polyclonal

Similar anti-Leptin Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 70 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies
Immunohistochemistry (IHC) 27 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 2 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Neutralization (Neut) 1 Antibodies
Radioimmunoassay (RIA) 3 Antibodies
Western Blotting (WB) 111 Antibodies

General

Antigen Leptin (LEP) Antibodies
Epitope AA 74-109, Middle Region
(27), (24), (19), (15), (15), (15), (11), (6), (6), (6), (6), (6), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(424), (170), (145), (22), (6), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(359), (215), (20), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(65), (23), (17), (11), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application ELISA, Western Blotting (WB)
(418), (415), (100), (56), (48), (39), (34), (26), (21), (19), (17), (10), (10), (7), (5), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Sequence KMDQTLAVYQ QVLTSLPSQN VLQIANDLEN LRDLLH
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Leptin(LEP) detection. Tested with WB, ELISA in Mouse.
Gene Name: leptin
Protein Name: Leptin
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of mouse Leptin (74-109aa KMDQTLAVYQQVLTSLPSQNVLQIANDLENLRDLLH), different from the related human sequence by five amino acids, and from the related rat sequence by two amino acids.
Isotype IgG

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Alternative Name LEP (LEP Antibody Abstract)
Background Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.
Gene ID 16846
UniProt P41160
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Leptin antibody (LEP) (AA 74-109) (ABIN3043294) Observed bind size: 16KD