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|Application / Reactivity||Mouse (Murine)|
|Blocking Antibody (Inhibition)||1 Antibodies|
|Enzyme Immunoassay (EIA)||10 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||7 Antibodies|
|Immunofluorescence (IF)||3 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||39 Antibodies|
|Immunohistochemistry (IHC)||27 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||22 Antibodies|
|Immunohistochemistry (Paraformaldehyde) (IHC (pfa))||2 Antibodies|
|Immunoprecipitation (IP)||10 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Radioimmunoassay (RIA)||3 Antibodies|
|Western Blotting (WB)||111 Antibodies|
|Antigen||Leptin (LEP) Antibodies|
|Epitope||AA 74-109, Middle Region Alternatives|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This Leptin antibody is un-conjugated Alternatives|
ELISA, Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-Leptin AntibodyTarget Details Leptin Application Details Handling Images
|Sequence||KMDQTLAVYQ QVLTSLPSQN VLQIANDLEN LRDLLH|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Leptin(LEP) detection. Tested with WB, ELISA in Mouse.
Gene Name: leptin
Protein Name: Leptin
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of mouse Leptin (74-109aa KMDQTLAVYQQVLTSLPSQNVLQIANDLENLRDLLH), different from the related human sequence by five amino acids, and from the related rat sequence by two amino acids.|
Target Details LeptinProduct Details anti-Leptin Antibody Application Details Handling Images back to top
|Alternative Name||LEP (LEP Antibody Abstract)|
|Background||Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.|
|Pathways||JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion|
Application DetailsProduct Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top