anti-Mouse (Murine) Leptin antibody for Immunohistochemistry (Paraformaldehyde)

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Reactivity
Mouse (Murine)
417
171
151
24
8
6
5
4
4
4
3
3
2
2
1
1
1
1
1
1
Host
Rabbit
367
207
19
8
4
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is conjugated to Biotin
63
26
17
14
10
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
ELISA, Immunohistochemistry (Paraformaldehyde) (IHC (pfa)), Western Blotting (WB)
406
399
108
57
56
39
31
31
30
26
17
10
10
6
5
4
4
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN465756
$ 493.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN465757 IHC (pfa) ELISA WB Rabbit Log in to see Polyclonal

Similar anti-Leptin Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 67 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies
Immunohistochemistry (IHC) 30 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 2 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Neutralization (Neut) 1 Antibodies
Radioimmunoassay (RIA) 3 Antibodies
Western Blotting (WB) 107 Antibodies

General

Antigen Leptin (LEP) Antibodies
Reactivity Mouse (Murine)
(417), (171), (151), (24), (8), (6), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(367), (207), (19), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is conjugated to Biotin
(63), (26), (17), (14), (10), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application ELISA, Immunohistochemistry (Paraformaldehyde) (IHC (pfa)), Western Blotting (WB)
(406), (399), (108), (57), (56), (39), (31), (31), (30), (26), (17), (10), (10), (6), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Specificity Mouse Leptin
Purification Immunoaffinity purified
Immunogen E.coli derived recombinant Murine Leptin.

Type of Immunogen: Cell extract

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Leptin (LEP Antibody Abstract)
Background Name/Gene ID: LEP

Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB
Gene ID 3952
UniProt P41159
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes Approved: ELISA (1 μg/mL), IHC-Pfa, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mLeptin by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin. To detect mLeptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine Leptin (LS-C104760) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin. Western Blot: To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Lyophilized
Reconstitution Sterile PBS containing 0.1 % BSA
Concentration Lot specific
Buffer Lyophilized from PBS, pH 7.2
Handling Advice Avoid freeze/thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
 image for anti-Leptin antibody (LEP)  (Biotin) (ABIN465756) anti-Leptin (LEP) antibody (Biotin)
 image for anti-Leptin antibody (LEP)  (Biotin) (ABIN465756) anti-Leptin (LEP) antibody (Biotin) (Image 2)