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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||107 Antibodies||263 Antibodies||89 Antibodies|
|Transfected Cell Culture (TCC)||1 Antibodies|
|Radioimmunoassay (RIA)||6 Antibodies||3 Antibodies||3 Antibodies|
|Protein Assay (PrA)||2 Antibodies|
|Neutralization (Neut)||2 Antibodies||1 Antibodies|
|Multiplex Assay (MA)||1 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Immunoprecipitation (IP)||20 Antibodies||9 Antibodies||2 Antibodies|
|Antigen||Leptin (LEP) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Leptin antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-Leptin AntibodyTarget Details Leptin Application Details Handling Images
|Specificity||This detects endogenous levels of Ob protein. (region surrounding Lys36)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % (by SDS-PAGE)|
Target Details LeptinProduct Details anti-Leptin Antibody Application Details Handling Images back to top
|Alternative Name||Leptin (LEP Antibody Abstract)|
|Background||OB Cadherin is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. OB Cadherin is expressed in osteoblasts and the brain as well as other tissues.Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor|
|Research Area||Cardiovascular, Atherosclerosis, Hormones|
|Pathways||JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process|
Application DetailsProduct Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top