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anti-Sheep (Ovine) Leptin antibody for Immunohistochemistry

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Reactivity
Bird (Avian), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
414
167
143
22
6
4
3
3
3
2
2
2
2
1
1
1
1
1
Host
Rabbit
353
211
19
8
4
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
64
23
17
11
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
409
404
97
56
46
39
33
26
20
19
17
10
9
6
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN152764
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1585862 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal

Similar anti-Leptin Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Sheep (Ovine) Pig (Porcine) Bird (Avian)
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 108 Antibodies 267 Antibodies 87 Antibodies 4 Antibodies 4 Antibodies 1 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Radioimmunoassay (RIA) 6 Antibodies 3 Antibodies 3 Antibodies 1 Antibodies
Protein Assay (PrA) 2 Antibodies
Neutralization (Neut) 2 Antibodies 1 Antibodies
Multiplex Assay (MA) 1 Antibodies

General

Antigen Leptin (LEP) Antibodies
Reactivity Bird (Avian), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(414), (167), (143), (22), (6), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(353), (211), (19), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(64), (23), (17), (11), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(409), (404), (97), (56), (46), (39), (33), (26), (20), (19), (17), (10), (9), (6), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Specificity Leptin
Purification Immunogen affinity purified
Immunogen Synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin.
Isotype IgG

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Leptin/OB (LEP Antibody Abstract)
Background Gene Symbol: LEP
Gene ID 3952
UniProt P41160
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes Western Blot 1:200 - 1:1000, Immunohistochemistry 1:50 - 1:200, Immunohistochemistry-Frozen 1:50 - 1:200, Immunocytochemistry 1:50 - 1:200WB: Detects an approx. 16 kDa protein representing leptin from 3T3-L1 cell extract. IHC: Staining of leptin in sheep brain with NB300-611 results in staining of the ventromedial hypothalamus.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Leptin Antibody Western Blot Protocol
1. Block with blotto 1 HR at RT
. Dilute primary 1:2000 - 1:4000 in blotto for 1 HR at RT
. Wash
. Secondary GAR-AP 1:2000 (or HRP) but dilution for secondary should be specific to detection method being used
. Wash
. Detect
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS and 1 mg/mL BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Leptin antibody (LEP) (ABIN152764) anti-Leptin (LEP) antibody
Immunohistochemistry (IHC) image for anti-Leptin antibody (LEP) (ABIN152764) Figure 2 shows staining of Leptin in sheep brain using ABIN152764.