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|Application / Reactivity||Rat (Rattus)||Human|
|ELISA||6 Antibodies||97 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||1 Antibodies|
|Immunofluorescence (IF)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||36 Antibodies||35 Antibodies|
|Immunohistochemistry (IHC)||5 Antibodies||46 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||13 Antibodies||30 Antibodies|
|Western Blotting (WB)||20 Antibodies|
|Enzyme Immunoassay (EIA)||7 Antibodies|
|Flow Cytometry (FACS)||5 Antibodies|
|Antigen||Leptin Receptor (LEPR) Antibodies|
|Epitope||AA 798-817, Middle Region Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This Leptin Receptor antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Leptin Receptor AntibodyTarget Details Leptin Receptor Application Details Handling Images
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Leptin receptor(LEPR) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: leptin receptor
Protein Name: Leptin receptor
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human Leptin Receptor(798-817aa HDHFIPIEKYQFSLYPIFME), different from the related mouse and rat sequences by two amino acids.|
Target Details Leptin ReceptorProduct Details anti-Leptin Receptor Antibody Application Details Handling Images back to top
|Alternative Name||LEPR (LEPR Antibody Abstract)|
|Background||LEPR(Leptin receptor) also known as LEP-R, OBR, is a protein that in humans is encoded by the LEPR gene. The leptin hormone regulates adipose-tissue mass through hypothalamus effects on fullness and energy use. It acts through the leptin receptor(LEP-R), a single-transmembrane-domain receptor of the cytokine receptor family. By genetic mapping, Tartaglia et al.(1995) demonstrated that the Obr gene is located on mouse chromosome 4, in a 5.1-cM interval that contains the well-characterized recessive obesity mutation, diabetes(db). Chung et al.(1996) developed a genetic map of 1p in the region of the OBR gene. They mapped the OBR gene physically by radiation hybrid mapping and placed it on a contig composed of 10 adjacent YACs and 5 P1 artificial chromosomes(PACs). The location of the human homolog of Obr was predicted to be on 1p based on conserved linkage of most of the telomeric half of mouse chromosome 4 with human 1p. LEP-R functions as a receptor for the fat cell-specific hormone leptin. LEP-R has also been designated as CD295.|
|Pathways||JAK-STAT Signaling, AMPK Signaling|
Application DetailsProduct Details anti-Leptin Receptor Antibody Target Details Leptin Receptor Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, The detection limit for LEPR is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-Leptin Receptor Antibody Target Details Leptin Receptor Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-Leptin Receptor Antibody Target Details Leptin Receptor Application Details Handling back to top