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anti-Rat (Rattus) Leptin Receptor antibody for Western Blotting

Recommended Leptin Receptor Antibody (supplied by: Log in to see )

Antigen
Leptin Receptor (LEPR) Antibodies
  • cd295
  • CD295
  • db
  • diabetes
  • Fa
  • LEP-R
  • LEPR
  • Leprb
  • LEPRD
  • LEPROT
  • Modb1
  • OB-R
  • Ob-R
  • ob-rb
  • OB-RGRP
  • Ob-RM
  • obese-like
  • obl
  • obr
  • OBR
  • Obr
  • ObRb
  • Obrgrp
  • VPS55
Epitope
AA 798-817, Middle Region
23
22
22
17
14
13
8
6
5
5
4
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
189
61
53
13
8
2
1
1
1
1
1
Host
Rabbit
138
41
26
2
2
1
Clonality
Polyclonal
Conjugate
This Leptin Receptor antibody is un-conjugated
9
9
8
6
6
5
4
4
4
4
3
3
3
3
3
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
130
106
51
34
34
13
7
7
4
2
Supplier
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Catalog No. ABIN3044107
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13384372 ABIN730303 IF (p) IHC (p) WB Rabbit IgG AA 540-590 Log in to see Polyclonal
0.13384372 ABIN1873534 IHC WB Rabbit IgG Log in to see Polyclonal
0.13384372 ABIN2426133 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.001175366 ABIN1582193 IHC ELISA WB Rabbit Log in to see Polyclonal 3
0.001175366 ABIN233311 ELISA WB Rabbit IgG AA 577-594 Log in to see Polyclonal
0.001175366 ABIN725060 IF (p) IHC (p) WB Rabbit IgG AA 905-942 Log in to see Polyclonal
0.001175366 ABIN725062 IHC (p) WB Biotin Rabbit IgG AA 905-942 Log in to see Polyclonal
0.001175366 ABIN725069 IHC (p) WB HRP Rabbit IgG AA 905-942 Log in to see Polyclonal
0.001175366 ABIN730305 IHC (p) WB Biotin Rabbit IgG AA 540-590 Log in to see Polyclonal
0.001175366 ABIN730312 IHC (p) WB HRP Rabbit IgG AA 540-590 Log in to see Polyclonal
0.001175366 ABIN2742559 WB Rabbit IgG AA 577-594, Extracellular Domain Log in to see Polyclonal
0.001175366 ABIN2299791 ELISA WB Rabbit IgG AA 577-594 Log in to see Polyclonal
0.001175366 ABIN1388197 IHC (p) WB Biotin Mouse IgG Log in to see Polyclonal
0.001175366 ABIN187816 WB Rabbit AA 577-594 Log in to see Polyclonal
0.001175366 ABIN1406090 IHC (p) WB HRP Mouse IgG Log in to see Polyclonal
0.001175366 ABIN1868964 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
0.001175366 ABIN1387629 IF (p) IHC (p) WB Mouse IgG Log in to see Polyclonal
0.001175366 ABIN1386273 IF (p) IHC (p) WB Mouse IgG Log in to see Polyclonal
0.001175366 ABIN3031601 IHC (p) WB Rabbit IgG Middle Region Log in to see Polyclonal

Top referenced anti-Leptin Receptor antibody for Western Blotting

Similar anti-Leptin Receptor Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 6 Antibodies 97 Antibodies
Flow Cytometry (FACS) 1 Antibodies 13 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 1 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies 34 Antibodies
Immunohistochemistry (IHC) 6 Antibodies 48 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies 32 Antibodies
Western Blotting (WB) 20 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies

General

Antigen Leptin Receptor (LEPR) Antibodies
Epitope AA 798-817, Middle Region
(23), (22), (22), (17), (14), (13), (8), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(189), (61), (53), (13), (8), (2), (1), (1), (1), (1), (1)
Host Rabbit
(138), (41), (26), (2), (2), (1)
Clonality
Polyclonal
Conjugate This Leptin Receptor antibody is un-conjugated
(9), (9), (8), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(130), (106), (51), (34), (34), (13), (7), (7), (4), (2)
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Product Details anti-Leptin Receptor Antibody

Target Details Leptin Receptor Application Details Handling Images
Sequence HDHFIPIEKY QFSLYPIFME
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Leptin receptor(LEPR) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: leptin receptor
Protein Name: Leptin receptor
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human Leptin Receptor(798-817aa HDHFIPIEKYQFSLYPIFME), different from the related mouse and rat sequences by two amino acids.
Isotype IgG

Target Details Leptin Receptor

Product Details anti-Leptin Receptor Antibody Application Details Handling Images back to top
Antigen
Alternative Name LEPR (LEPR Antibody Abstract)
Background LEPR(Leptin receptor) also known as LEP-R, OBR, is a protein that in humans is encoded by the LEPR gene. The leptin hormone regulates adipose-tissue mass through hypothalamus effects on fullness and energy use. It acts through the leptin receptor(LEP-R), a single-transmembrane-domain receptor of the cytokine receptor family. By genetic mapping, Tartaglia et al.(1995) demonstrated that the Obr gene is located on mouse chromosome 4, in a 5.1-cM interval that contains the well-characterized recessive obesity mutation, diabetes(db). Chung et al.(1996) developed a genetic map of 1p in the region of the OBR gene. They mapped the OBR gene physically by radiation hybrid mapping and placed it on a contig composed of 10 adjacent YACs and 5 P1 artificial chromosomes(PACs). The location of the human homolog of Obr was predicted to be on 1p based on conserved linkage of most of the telomeric half of mouse chromosome 4 with human 1p. LEP-R functions as a receptor for the fat cell-specific hormone leptin. LEP-R has also been designated as CD295.
UniProt P48357
Pathways JAK-STAT Signaling, AMPK Signaling

Application Details

Product Details anti-Leptin Receptor Antibody Target Details Leptin Receptor Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, The detection limit for LEPR is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-Leptin Receptor Antibody Target Details Leptin Receptor Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-Leptin Receptor Antibody Target Details Leptin Receptor Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Leptin Receptor antibody (LEPR) (AA 798-817) (ABIN3044107) Anti-Leptin Receptor antibody, Western blotting Lane 1: Rat Heart Tissue Lysate Lane ...
Immunohistochemistry (IHC) image for anti-Leptin Receptor antibody (LEPR) (AA 798-817) (ABIN3044107) Anti-Leptin Receptor antibody, IHC(P) IHC(P): Rat Brain Tissue