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anti-Human LIF antibody for Flow Cytometry

Recommended LIF Antibody (supplied by: Log in to see )

Antigen
Leukemia Inhibitory Factor (LIF) Antibodies
  • A230075M04Rik
  • AW061234
  • CDF
  • DIA
  • HILDA
  • LIF
  • MLPLI
Epitope
AA 72-101, Center
15
13
8
7
5
5
4
3
3
3
2
2
2
1
1
1
1
1
Reactivity
Human
113
64
24
6
6
4
4
Host
Rabbit
90
22
22
3
2
Clonality
Polyclonal
Conjugate
This LIF antibody is un-conjugated
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
110
41
34
28
26
6
5
4
3
3
2
1
1
Supplier
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Catalog No. ABIN390969
€ 309.05
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3040498 ABIN2665228 FACS Rat IgG2a Log in to see M1506B09 4
0.3040498 ABIN2444808 FACS IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.0010469046 ABIN453168 EIA FACS IHC (p) WB Rabbit AA 79-109 Log in to see Polyclonal 1
0.0010469046 ABIN1909809 FACS IHC ELISA WB FITC Rabbit IgG AA 79-109 Log in to see Polyclonal
0.0010469046 ABIN1909806 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 79-109 Log in to see Polyclonal
0.0010469046 ABIN1909808 FACS IHC ELISA WB Biotin Rabbit IgG AA 79-109 Log in to see Polyclonal
0.0010469046 ABIN1909807 FACS IHC ELISA WB APC Rabbit IgG AA 79-109 Log in to see Polyclonal
0.0010469046 ABIN1909810 FACS IHC ELISA WB PE Rabbit IgG AA 79-109 Log in to see Polyclonal
0.0010469046 ABIN3021874 FACS IHC WB Rabbit IgG Log in to see Polyclonal
0.0010469046 ABIN1909811 FACS IHC ELISA WB HRP Rabbit IgG AA 79-109 Log in to see Polyclonal
0.0010469046 ABIN2624892 FACS IHC ELISA WB Rabbit IgG AA 79-109 Log in to see Polyclonal
0.0010469046 ABIN2299943 FACS IHC ELISA WB Rabbit IgG AA 79-109, Center Log in to see Polyclonal
0.0010469046 ABIN2299951 FACS IHC ELISA WB FITC Rabbit IgG AA 79-109, Center Log in to see Polyclonal
0.0010469046 ABIN2299958 FACS IHC ELISA WB APC Rabbit IgG AA 79-109, Center Log in to see Polyclonal
0.0010469046 ABIN2299964 FACS IHC ELISA WB PE Rabbit IgG AA 79-109, Center Log in to see Polyclonal
0.0010469046 ABIN2299945 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 79-109, Center Log in to see Polyclonal
0.0010469046 ABIN2299960 FACS IHC ELISA WB Biotin Rabbit IgG AA 79-109, Center Log in to see Polyclonal
0.0010469046 ABIN1818294 FACS IP Mouse IgG1 Log in to see 1C7
0.0010469046 ABIN1818295 FACS IP Mouse IgG1 Log in to see 12D3
0.0010469046 ABIN4330947 FACS Alexa Fluor 488 Mouse IgG1 Log in to see 9808

Top referenced anti-LIF antibody for Flow Cytometry

  • Human Polyclonal LIF Primary Antibody for EIA, FACS - ABIN453168 (1 Publications): Novotný, Krízan, Síma, Síma, Uher, Zech, Hütelová, Baborová, Ulcová-Gallová, Subrt, Ulmanová, Houdek, Rokyta, Babuska, Králícková: Leukaemia inhibitory factor (LIF) gene mutations in women diagnosed with unexplained infertility and endometriosis have a negative impact on the IVF outcome. A pilot study. in Folia biologica 2009 (PubMed)

Similar anti-LIF Antibodies

Application / Reactivity Human
ELISA 31 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 29 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 26 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Neutralization (Neut) 2 Antibodies
Western Blotting (WB) 92 Antibodies

General

Antigen Leukemia Inhibitory Factor (LIF) Antibodies
Epitope AA 72-101, Center
(15), (13), (8), (7), (5), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(113), (64), (24), (6), (6), (4), (4)
Host Rabbit
(90), (22), (22), (3), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This LIF antibody is un-conjugated
(4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(110), (41), (34), (28), (26), (6), (5), (4), (3), (3), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-LIF Antibody

Target Details LIF Application Details Handling References for anti-LIF Antibody (ABIN390969) Images
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This LIF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-101 AA from the Central region of human LIF.
Clone RB21725
Isotype Ig

Target Details LIF

Product Details anti-LIF Antibody Application Details Handling References for anti-LIF Antibody (ABIN390969) Images back to top
Antigen
Alternative Name LIF (LIF Antibody Abstract)
Background LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.

Synonyms: Leukemia inhibitory factor, LIF, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor, MLPLI, INN=Emfilermin, Flags: Precursor, HILDA, LIF
Molecular Weight 22008 Da
Gene ID 3976
UniProt P15018
Research Area Neurology
Pathways JAK-STAT Signaling

Application Details

Product Details anti-LIF Antibody Target Details LIF Handling References for anti-LIF Antibody (ABIN390969) Images back to top
Application Notes Suggested dilutions:
WB = 1:1000, IHC (p) = 1:10-50, FACS = 1:10-50
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-LIF Antibody Target Details LIF Application Details References for anti-LIF Antibody (ABIN390969) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months

References for anti-LIF Antibody (ABIN390969)

Product Details anti-LIF Antibody Target Details LIF Application Details Handling Images back to top
Background publications

Novotný, Krízan, Síma, Síma, Uher, Zech, Hütelová, Baborová, Ulcová-Gallová, Subrt, Ulmanová, Houdek, Rokyta, Babuska, Králícková: "Leukaemia inhibitory factor (LIF) gene mutations in women diagnosed with unexplained infertility and endometriosis have a negative impact on the IVF outcome. A pilot study." in: Folia biologica, Vol. 55, Issue 3, pp. 92-7, 2009

Images

Product Details anti-LIF Antibody Target Details LIF Application Details Handling References for anti-LIF Antibody (ABIN390969) back to top
Supplier Images
Western Blotting (WB) image for anti-LIF antibody (Leukemia Inhibitory Factor) (AA 72-101) (ABIN390969) Western blot analysis of LIF Antibody (Center) (ABIN390969) in CEM cell line lysates ...
Immunohistochemistry (IHC) image for anti-LIF antibody (Leukemia Inhibitory Factor) (AA 72-101) (ABIN390969) Formalin-fixed and paraffin-embedded human colon carcinoma reacted with LIF Antibody ...
Flow Cytometry (FACS) image for anti-LIF antibody (Leukemia Inhibitory Factor) (AA 72-101) (ABIN390969) Flow cytometric analysis of CEM cells using LIF Antibody (Center)(bottom histogram) c...