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anti-Human LIF antibody for Immunocytochemistry

Recommended LIF Antibody (supplied by: Log in to see )

Antigen
Leukemia Inhibitory Factor (LIF) Antibodies
  • A230075M04Rik
  • AW061234
  • CDF
  • DIA
  • HILDA
  • LIF
  • MLPLI
Epitope
AA 2-201
15
14
8
7
5
5
4
3
3
3
2
2
2
1
1
1
1
1
Reactivity
Human
113
64
24
6
6
4
4
Host
Rabbit
90
22
22
3
2
Clonality
Polyclonal
Conjugate
This LIF antibody is un-conjugated
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
110
40
34
29
26
5
5
3
3
3
2
1
1
Supplier
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Catalog No. ABIN1859653
$ 246.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010086668 ABIN4330945 ELISA ICC IF WB Rabbit Center Log in to see Polyclonal
0.0010086668 ABIN4330946 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal

Similar anti-LIF Antibodies

Application / Reactivity Human
ELISA 31 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 29 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 26 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Neutralization (Neut) 2 Antibodies
Western Blotting (WB) 92 Antibodies

General

Antigen Leukemia Inhibitory Factor (LIF) Antibodies
Epitope AA 2-201
(15), (14), (8), (7), (5), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(113), (64), (24), (6), (6), (4), (4)
Host Rabbit
(90), (22), (22), (3), (2)
Clonality
Polyclonal
Conjugate This LIF antibody is un-conjugated
(4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(110), (40), (34), (29), (26), (5), (5), (3), (3), (3), (2), (1), (1)
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Product Details anti-LIF Antibody

Target Details LIF Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KVLAAGVVP LLLVLHWKHG AGSPLPITPV NATCAIRHPC HNNLMNQIRS QLAQLNGSAN ALFILYYTAQ GEPFPNNLDK LCGPNVTDFP PFHANGTEKA KLVELYRIVV YLGTSLGNIT RDQKILNPSA LSLHSKLNAT ADILRGLLSN VLCRLCSKYH VGHVDVTYGP DTSGKDVFQK KKLGCQLLGK YKQIIAVLAQ A
Specificity It has been selected for its ability to recognize LIF in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen LIF (AA 2-201)
Isotype IgG

Target Details LIF

Product Details anti-LIF Antibody Application Details Handling Images back to top
Antigen
Research Area Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-LIF Antibody Target Details LIF Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant LIF (Lys2~Ala201) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-LIF Antibody Target Details LIF Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-LIF Antibody Target Details LIF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-LIF antibody (Leukemia Inhibitory Factor) (AA 2-201) (ABIN1859653) anti-Leukemia Inhibitory Factor (LIF) (AA 2-201) antibody