anti-Human LIF antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended LIF Antibody (supplied by: Log in to see )

Antigen
Leukemia Inhibitory Factor (LIF) Antibodies
  • CDF
  • DIA
  • HILDA
  • MLPLI
  • A230075M04Rik
  • AW061234
  • LIF
  • leukemia inhibitory factor
  • LIF, interleukin 6 family cytokine
  • leukemia inhibitory factor receptor
  • Lif
  • LIF
  • Lifr
Epitope
AA 79-109
15
14
9
7
6
6
4
4
4
3
3
3
3
2
1
1
1
1
1
1
Reactivity
Human
139
84
31
11
7
6
5
2
2
2
1
1
Host
Rabbit
95
39
37
6
2
2
Clonality
Polyclonal
Conjugate
This LIF antibody is un-conjugated
7
5
5
5
5
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
138
47
45
45
34
6
6
4
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN453168
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.221225 ABIN737046 IF (p) IHC (p) WB Rabbit IgG AA 177-202 Log in to see Polyclonal 1
14.221225 ABIN4330955 IHC (p) WB Rat IgG2b kappa Log in to see 39N7D10
1 ABIN3043403 IHC (p) WB Rabbit IgG AA 23-202 Log in to see Polyclonal 5
1 ABIN390969 FACS IHC (p) WB Rabbit Ig AA 72-101, Center Log in to see Polyclonal 1
1 ABIN3180980 ELISA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN959656 IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN5077820 IHC (p) WB Rat IgG2b kappa AA 24-203 Log in to see 39N7D10
1 ABIN4330954 IHC (p) WB Rat IgG2b kappa Log in to see 39N7D10
1 ABIN1736440 IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN2624891 IHC (p) WB Rabbit IgG AA 23-202 Log in to see Polyclonal
1 ABIN2742599 IHC (p) WB Rabbit IgG AA 23-202 Log in to see Polyclonal
1 ABIN4330946 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN3028797 IHC (p) WB Rabbit IgG AA 23-202 Log in to see Polyclonal
1 ABIN4229718 IHC (p) WB Alexa Fluor 700 Rat IgG2b kappa Log in to see 39N7D10
1 ABIN4229703 IHC (p) WB Biotin Rat IgG2b kappa Log in to see 39N7D10
1 ABIN4229704 IHC (p) WB DyLight 650 Rat IgG2b kappa Log in to see 39N7D10
1 ABIN4229708 IHC (p) WB DyLight 755 Rat IgG2b kappa Log in to see 39N7D10
1 ABIN4229709 IHC (p) WB PerCP Rat IgG2b kappa Log in to see 39N7D10
1 ABIN4229711 IHC (p) WB DyLight 550 Rat IgG2b kappa Log in to see 39N7D10
1 ABIN4229712 IHC (p) WB DyLight 350 Rat IgG2b kappa Log in to see 39N7D10

General

Antigen Leukemia Inhibitory Factor (LIF) Antibodies
Epitope AA 79-109
(15), (14), (9), (7), (6), (6), (4), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(139), (84), (31), (11), (7), (6), (5), (2), (2), (2), (1), (1)
Host Rabbit
(95), (39), (37), (6), (2), (2)
Clonality
Polyclonal
Conjugate This LIF antibody is un-conjugated
(7), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(138), (47), (45), (45), (34), (6), (6), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-LIF Antibody

Target Details LIF Application Details Handling References for anti-LIF antibody (ABIN453168) Images
Specificity This antibody reactcs to Leukemia inhibitory factor
Purification Protein A column, followed by peptide affinity purification
Immunogen KLH conjugated synthetic peptide between 79~109 amino acids from the Center region of Human LIF

Target Details LIF

Product Details anti-LIF Antibody Application Details Handling References for anti-LIF antibody (ABIN453168) Images back to top
Antigen
Alternative Name Leukemia Inhibitory Factor (LIF Antibody Abstract)
Background LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.Synonyms: D factor, Differentiation-stimulating factor, Emfilermin, HILDA, LIF, MLPLI, Melanoma-derived LPL inhibitor
Gene ID 3976
NCBI Accession NP_002300
UniProt P15018
Research Area Cancer
Pathways JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding

Application Details

Product Details anti-LIF Antibody Target Details LIF Handling References for anti-LIF antibody (ABIN453168) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry on paraffin sections: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-LIF Antibody Target Details LIF Application Details References for anti-LIF antibody (ABIN453168) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-LIF antibody (ABIN453168)

Product Details anti-LIF Antibody Target Details LIF Application Details Handling Images back to top
Background publications

Novotný, Krízan, Síma, Síma, Uher, Zech, Hütelová, Baborová, Ulcová-Gallová, Subrt, Ulmanová, Houdek, Rokyta, Babuska, Králícková: "Leukaemia inhibitory factor (LIF) gene mutations in women diagnosed with unexplained infertility and endometriosis have a negative impact on the IVF outcome. A pilot study." in: Folia biologica, Vol. 55, Issue 3, pp. 92-7, 2009

Images

Product Details anti-LIF Antibody Target Details LIF Application Details Handling References for anti-LIF antibody (ABIN453168) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Leukemia Inhibitory Factor (LIF) (AA 79-109) antibody (ABIN453168) Formalin-fixed and paraffin-embedded human colon carcinoma reacted with LIF Antibody ...
Western Blotting (WB) image for anti-Leukemia Inhibitory Factor (LIF) (AA 79-109) antibody (ABIN453168) Western blot analysis of LIF Antibody (Center) in CEM cell line lysates (35ug/lane). ...